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Membrane trafficking

Lipid sorting and clustering

Nature Reviews Molecular Cell Biology volume 10, pages 442–443 (2009)Cite this article

The authors engineered the expression of a green fluorescent protein (GFP)-tagged form of a transmembrane raft protein, FusMid, for the immunopurification of TGN-derived secretory vesicles from live yeast cells. Likewise, an engineered TGN/endosomal protein, Gap1–GFP, was used for purification of the donor compartment. Electron microscopy and western blot analyses revealed that these two subcellular compartments are morphologically distinct and have different protein compositions, and comparative quantitative lipidomics further uncovered their differential membrane constituents. The authors found that ergosterol and the sphingolipid mannosyl-di-IPC (M(IP)2C) are enriched in FusMid vesicles compared with the TGN/endosomes, which suggests that lipid sorting occurs at the TGN. The enrichment of these two lipid raft-associated lipid species further indicates that a raft-clustering mechanism might be involved in the formation of secretory vesicles.

...sorting of plasma membrane lipids occurs at the TGN.

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References

ORIGINAL RESEARCH PAPER

  1. Klemm, R. W. et al. Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network. J. Cell Biol. 11 May 2009 (doi:10.1083/jcb.200901145)

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FURTHER READING

  1. van Meer, G. et al. Membrane lipids: where they are and how they behave. Nature Rev. Mol. Cell Biol. 9, 112–124 (2008)

    Article  CAS  Google Scholar 

  2. Ikonen, E. Cellular cholesterol trafficking and compartmentalization. Nature Rev. Mol. Cell Biol. 9, 125–138 (2008)

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Authors
  1. Alicia Chung
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Chung, A. Lipid sorting and clustering. Nat Rev Mol Cell Biol 10, 442–443 (2009). https://doi.org/10.1038/nrm2711

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