Abstract
RNA modifications can alter RNA structure–function relationships and various cellular processes. However, the genomic distribution and biological roles of most RNA modifications remain uncharacterized. Here, we propose using phage display antibody technology and direct sequencing through nanopores to facilitate systematic interrogation of the distribution, location and dynamics of RNA modifications.
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References
Jia, G. et al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO. Nat. Chem. Biol. 7, 885–887 (2011).
Saletore, Y. et al. The birth of the Epitranscriptome: deciphering the function of RNA modifications. Genome Biology 13, 175 (2012).
Meyer, K. D. et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons. Cell 149, 1635–1646 (2012).
Dominissini, D. et al. Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485, 201–206 (2012).
Hauenschild, R. et al. The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent. Nucleic Acids Res. 43, 9950–9964 (2015).
Lee, C. M., Iorno, N., Sierro, F. & Christ, D. Selection of human antibody fragments by phage display. Nat. Protoc. 2, 3001–3008 (2007).
Simpson, J. T. et al. Detecting DNA cytosine methylation using nanopore sequencing. Nat. Methods 14, 407–410 (2017).
Garalde, D. R. et al. Highly parallel direct RNA sequencing on an array of nanopores. bioRxiv https://doi.org/10.1101/068809 (2016).
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Novoa, E., Mason, C. & Mattick, J. Charting the unknown epitranscriptome. Nat Rev Mol Cell Biol 18, 339–340 (2017). https://doi.org/10.1038/nrm.2017.49
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DOI: https://doi.org/10.1038/nrm.2017.49
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