A new method has been developed to analyse intra-host genetic diversity of malaria infections from patient blood. Malaria infections are often comprised of a complex assortment of parasite lineages — also known as multiple-genotype infections. In this study, cell sorting was followed by whole-genome amplification to generate sufficient DNA from infected red blood cells for subsequent genotyping and sequencing analyses. Better understanding the impact of various combinations of intra-host drug resistance and virulence haplotypes could facilitate more directed treatment strategies in the future.
References
Nair, S. et al. Single-cell genomics for dissection of complex malaria infections. Genome Res. http://dx.doi.org/10.1101/gr.168286.113 (2014)
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Jones, B. Single-cell analysis of intra-host variation. Nat Rev Genet 15, 441 (2014). https://doi.org/10.1038/nrg3770
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DOI: https://doi.org/10.1038/nrg3770