Previously, the identification of replication origins (ORIs) in metazoans has been hampered by the lack of stringent and sensitive genome-wide techniques for identifying these sites. The authors here present a genome-wide map of human ORIs obtained by chromatin immunoprecipitation followed by sequencing (ChIP–seq) against ORC1 (a crucial component of the origin recognition complex). Human ORIs were found to be associated with transcription start sites. The timing of firing of these ORIs was linked to the level of transcription: higher transcription was associated with earlier firing in S phase.
ORIGINAL RESEARCH PAPER
Dellino, G. I. et al. Genome-wide mapping of human DNA-replication origins: levels of transcription at ORC1 sites regulate origin selection and replication timing. Genome Res. 27 Nov 2012 (doi:10.1101/gr.142331.112)
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Stower, H. ChIP–seq for human replication origins. Nat Rev Genet 14, 78 (2013). https://doi.org/10.1038/nrg3417
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DOI: https://doi.org/10.1038/nrg3417
