Haploid embryonic stem cells have been previously derived and shown to be amenable to genetic analysis. This paper describes the derivation of mouse androgenetic haploid embryonic stem cells (AG-haESCs) that can be used to derive live mice; the use of haploid cells might provide a simple way to generate genetically manipulated animals. AG-haESCs maintain paternal imprints and contribute to multiple tissues when injected into diploid blastocysts. Injection of AG-haESCs into MII oocytes also leads to fertile offspring, and AG-haESCs can be genetically manipulated.
ORIGINAL RESEARCH PAPER
Yang, H. et al. Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. Cell 149, 605–617 (2012)
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Stower, H. Mouse genetically tractable fertilization agent. Nat Rev Genet 13, 378 (2012). https://doi.org/10.1038/nrg3259
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DOI: https://doi.org/10.1038/nrg3259
