Two papers present approaches for direct identification of the co-occurrence of histone modifications and DNA methylation; the relationship between these marks is usually studied by correlative approaches. In both papers, the authors used chromatin immunoprecipitation to capture the fraction of the genome that is associated with the histone modification of interest — in this case, histone H3 lysine 27 trimethylation — and then performed bisulphite sequencing on the immunoprecipitated material to map DNA methylation at high resolution. This approach should be applicable to other histone marks.
ORIGINAL RESEARCH PAPERS
Statham, A. L. et al. Bisulphite-sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA. Genome Res. 30 Mar 2012 (doi:10.1101/gr.132076.111)
Brinkman, A. B. et al. Sequential ChIP-bisulfite sequencing enables direct genome-scale investigation of chromatin and DNA methylation cross-talk. Genome Res. 30 Mar 2012 (doi:10.1101/gr.133728.111)
Rights and permissions
About this article
Cite this article
Muers, M. Mapping histone modifications and DNA methylation together. Nat Rev Genet 13, 299 (2012). https://doi.org/10.1038/nrg3235
Published:
Issue Date:
DOI: https://doi.org/10.1038/nrg3235