Proper control of the transcriptome is key for diverse aspects of gene expression, cellular functions and development, and its disruption can result in disease. A rapidly accumulating wealth of studies are identifying and functionally characterizing diverse types of RNA base modifications in protein-coding and non-coding RNAs, which have energized the emerging field of 'epitranscriptomics'. In this Viewpoint article, five experts discuss our latest understanding of RNA modifications, including recommendations for best practices and visions for the future.
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S.R.J. is supported by US National Institutes of Health (NIH) grants R01DA037755 and R01CA186702. T.P. is supported by NIH grant R01GM113194. G.R. acknowledges D.Dominissini, S. Moshitch-Moshkovitz and N. Amariglio for helpful discussions and comments. G.R is supported by the Kahn Family Foundation, the Flight Attendant Medical Research Institute (FAMRI), Israel Science Foundation (ISF grant no. 1667/12), Israeli Centers of Excellence (I-CORE) Program (ISF grants no. 41/11 and no. 1796/12), the Sagol Neuroscience Network and the Teva National Network of Excellence in Neuroscience. T.S. is supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
The authors declare no competing financial interests.
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Frye, M., Jaffrey, S., Pan, T. et al. RNA modifications: what have we learned and where are we headed?. Nat Rev Genet 17, 365–372 (2016). https://doi.org/10.1038/nrg.2016.47
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