Inhibitory synaptic transmission in the amygdala has a pivotal role in fear learning and its extinction. However, the local circuits formed by GABAergic inhibitory interneurons within the amygdala and their detailed function in shaping these behaviors are not well understood. Here we used lentiviral-mediated knockdown of the cell adhesion molecule neurofascin in the basolateral amygdala (BLA) to specifically remove inhibitory synapses at the axon initial segment (AIS) of BLA projection neurons. Quantitative analysis of GABAergic synapse markers and measurement of miniature inhibitory postsynaptic currents in BLA projection neurons after neurofascin knockdown ex vivo confirmed the loss of GABAergic input. We then studied the impact of this manipulation on anxiety-like behavior and auditory cued fear conditioning and its extinction as BLA related behavioral paradigms, as well as on long-term potentiation (LTP) in the ventral subiculum–BLA pathway in vivo. BLA knockdown of neurofascin impaired ventral subiculum–BLA–LTP. While this manipulation did not affect anxiety-like behavior and fear memory acquisition and consolidation, it specifically impaired extinction. Our findings indicate that modification of inhibitory synapses at the AIS of BLA projection neurons is sufficient to selectively impair extinction behavior. A better understanding of the role of distinct GABAergic synapses may provide novel and more specific targets for therapeutic interventions in extinction-based therapies.
The amygdala is a key brain region that in conjunction with the hippocampus and the prefrontal cortex supports fear memory formation and extinction (McGaugh, 2004; Milad and Quirk, 2002; Pape and Pare, 2010). While an extensive body of research has mostly focused on studying the role of excitatory synapses in fear memory formation, cumulative evidence suggests an important role of inhibitory synapses in modulating fear memory in the amygdala (Ehrlich et al, 2009; Lee et al, 2013). The interplay between excitatory principal cells and interneurons mediates local circuit activity (Maroun and Richter-Levin, 2002; Isaacson and Scanziani, 2011). Similarly to cortical structures, 10–15% of the neuronal population of the basolateral amygdala (BLA) is composed of a diverse group of GABAergic interneurons, which innervate distinct subcellular domains of projection neurons and are thought to shape fear memory (Ehrlich et al, 2009; Li et al, 1996; Mascagni and McDonald, 2003; Spampanato et al, 2011). Recent studies have begun to reveal a critical role of specific interneuron subpopulations in the BLA in different phases of fear memory formation and extinction (Bienvenu et al, 2012; Wolff et al, 2014). For instance, axo-axonic cells (AACs) are recruited in response to noxious stimuli and contribute also to the integration of hippocampal theta oscillation (Bienvenu et al, 2012), which is part of the memory consolidation process and is engaged during extinction learning (Lesting et al, 2011; Seidenbecher et al, 2003). During fear extinction, subsets of BLA projection neurons activated by fear conditioning are silenced by increased perisomatic inhibition originating from parvalbumin-positive interneurons (Trouche et al, 2013).
One possibility to control GABAergic inhibitory transmission of AACs emerges by manipulating neurofascin (NF) (Kriebel et al, 2011; Zitman et al, 2014). Neurofascin is a cell adhesion molecule belonging to the L1 subfamily of immunoglobulins (Kriebel et al, 2012). A neuronal isoform of NF is exclusively expressed at the innervation area of AACs, the axon initial segment (AIS). There, NF has been implicated in maintaining action potential initiation (Zonta et al, 2011) and in stabilizing inhibitory synapses by regulating the postsynaptic scaffold protein gephyrin (Burkarth et al, 2007; Kriebel et al, 2011). Knockdown of NF at the AIS decreased gephyrin clustering, which was associated with reduced expression of the GABAA receptor subunit β2/3 at the postsynaptic site and GAD65 at the inhibitory presynapse (Zitman et al, 2014). Within the dentate gyrus (DG), such a modulation of GABAergic inhibitory transmission at the AIS via NF knockdown altered feedback inhibition measured by paired-pulse stimulation while feed-forward inhibition remained unchanged. This finding suggests a role of NF in specific aspects of local circuit activity. In addition, NF knockdown in the DG impaired avoidance learning in the two-way shuttle box, thus highlighting a functional role in stress coping (Zitman et al, 2014).
In the present study, we investigated how reduction of GABAergic synapses at the AIS of BLA projection neurons by NF knockdown affects cued fear conditioning and extinction as key paradigms recruiting the BLA. We assessed long-term potentiation (LTP) in the ventral subiculum (vSub)–BLA pathway, which represents the major connection between hippocampus and BLA as part of the fear conditioning and extinction network (Biedenkapp and Rudy, 2009; Hobin et al, 2006; Horovitz and Richter-Levin, 2015; Kim et al, 2013; Maren et al, 1995). Indeed, LTP was impaired after NF knockdown, confirming the functionality of the manipulation. While cued fear memory was intact, rats with NF knockdown displayed a selective deficit in fear extinction.
Materials and methods
Neurofascin knockdown was achieved via lentiviral miRNA expression vectors directed against all rat NF isoforms. Two target sequences were chosen (miNF1196 and miNF1707) and were cloned into vectors that provide the expression of miRNA sequences under the control of a short mouse CaMKII promoter and enhanced green fluorescent protein (EGFP) as a marker under the control of a short rat synapsin promoter (Dittgen et al, 2004). The miRNA-mediated reduction of NF expression was first validated in vitro in rat primary cortical neurons by comparing NF mRNA expression levels via quantitative PCR. We then proceeded to inject viral vectors bilaterally into the BLA (Figure 1c–e) of young adult (post-natal day (PND) 60) male Sprague–Dawley rats. After recovery from stereotactic injection for 2 weeks, either immunohistochemical and electrophysiological validation of the NF knockdown effect in vivo was performed in slice preparations, or rats were subjected to a battery of behavioral tests and subsequent in vivo electrophysiology (see experimental timeline Figure 1f), which was finalized by validation of injection and recording sites (Figure 1c–e; Supplementary Figures S6 and S9).
Immunohistochemical analysis (PND 74) comprised the identification of RNAi expressing neurons in the BLA via EGFP co-expression, followed by immunostainings for either Ankyrin G (AnkG) together with vGAT or gephyrin, or AnkG together with NF, which were always combined with blue fluorescent Nissl staining. Images were obtained with a laser-scanning microscope. Image analysis was performed in three dimensions on Z-stacks of quadruple fluorescence images. We quantified the lentiviral-induced knockdown of NF and the number of GABAergic synapses by pre- and postsynaptic markers (vGAT or gephyrin, respectively) on AISs (using AnkG as a marker), and in addition, vGAT terminals on the somata of BLA projection neurons.
Slice electrophysiology (PND 81) was performed using standard procedures to assess cellular excitability and inhibitory synaptic transmission by measuring miniature inhibitory postsynaptic currents (mIPSCs) after NF knockdown in BLA projection neurons.
The behavioral analysis was comprised of an open-field (OFT; PND 74) and an elevated plus maze (EPM; PND 75) test (Ardi et al, 2016), followed by fear conditioning (PND 76–81) to an auditory cue and extinction by re-exposure to tones on 3 consecutive post-training days (adapted from Vouimba and Maroun, 2011).
In vivo electrophysiology (PND 82) was performed to test whether a high-frequency stimulation (HFS) of the vSub can induce plasticity in the BLA (adapted from Horovitz and Richter-Levin, 2015).
All methods are described in detail in the Supplementary Information.
In Vitro Validation of Neurofascin Knockdown
To specifically remove NF from projection neurons in the BLA, we designed lentiviral vectors that expressed miRNAs directed against NF or a control miRNA under control of a truncated CaMKII promoter together with EGFP controlled by a synapsin promoter (Figure 1a). To assess the efficacy of lentiviral constructs used for miRNA-mediated knockdown of NF, we quantitatively transduced primary cortical rat neurons with respective viral suspensions (Supplementary Figure S1). Expression of two different miRNAs specific for NF yielded markedly reduced levels of NF mRNA measured by quantitative real-time PCR when compared with control miRNA (CTR)-transduced cultures (experiments carried out in duplicate), indicating highly efficient suppression of NF mRNA expression (Figure 1b). The NF1707 miRNA (NF1707) construct appeared to be slightly more effective than the NF1196 miRNA (NF1196) construct.
In Vivo Knockdown of Neurofascin Reduces GABAergic Input at the Axon Initial Segment
To verify that lenitiviral knockdown of NF mRNA reduced its protein levels in vivo, quantitative immunohistochemistry was performed 2 weeks after viral injection (Supplementary Figure S2). We compared non-infected (EGFP-negative) with close-by infected (EGFP-positive) BLA projection neurons within the same reconstructed Z-stack after confocal image acquisition (Figure 2a–c). Using AnkG as a marker, we confirmed that the lengths of the AISs were similar in infected and non-infected neurons for all viral constructs (CTR, t(46)=−1.87, P=0.068; NF1196, t(40)=−0.79, P=0.433; NF1707, t(38)=−0.343, P=0.735; Figure 2d), indicating no major structural impairment of the AIS. Knockdown with NF1707 led to a significant reduction in NF staining at the AIS (t(19)=−3.493, P=0.003), while the CTR was ineffective (t(24)=−0.577, P=0.569). No reduction of NF signal was observed with NF1196 (t(20)=−0.323, P=0.750), although this construct reduced mRNA levels, suggesting that the reduction in protein expression was too weak to detect by immunostaining (Figure 2e). In a next step, we assessed the impact of NF knockdown on the density of presynaptic vesicular GABA transporter clusters used as marker for GABAergic terminals (Chaudhry et al, 1998). We found that loss of NF with NF1707 was accompanied by a highly significant reduction of vGAT-positive terminals at the AIS (by 38% (t(19)=7.011, P<0.0001), which did not occur with CTR expression (t(22)=0.729, P=0.474; Figure 2f). There was also a smaller, yet significant decrease of vGAT terminals at the AIS after expression of NF1196 by 13% (t(20)=3.179, P=0.005; Figure 2f), confirming the partial efficacy of this construct. The overall coverage and distribution of vGAT-positive terminals at the AIS was in good agreement with previously published data (cf. Supplementary Figure S3; Veres et al, 2014). The relative distribution was not altered by miRNA expression, indicating that vGAT terminals were uniformly reduced along the AIS (Supplementary Figure S3). Importantly, GABAergic terminals at neuronal somata were not altered and also did not differ between the viral constructs used (F(2,44)=0.136, P=0.873) (Figure 2g and h), indicating that perisomatic GABAergic synapses were unaffected. In addition, we used gephyrin as postsynaptic marker of GABAergic synapses (Craig et al, 1996) to confirm loss of synapses. Again, AnkG staining confirmed that the lengths of the AISs were unaltered with viral transduction (CTR vs non-inf., t(8)=0.326, P=0.754; NF1707 vs non-inf., t(12)=-0.223, P=0.828; Supplementary Figure S4c), indicating no major structural impairment of the AIS. Loss of NF with expression of NF1707 led to a significant reduction of gephyrin clusters along the length of the AIS by 30% (vs non-inf., t(12)=−0.793; P<0.001; Supplementary Figure S4b, d and e), which did not occur with CTR expression (vs non-inf. t(8)=−6.595; P=0.454; Supplementary Figure S4a, d and e).
Together, our data show that two independent NF-specific miRNAs impaired GABAergic innervation at the AIS, but not at the soma, suggesting no off-target effects. However, in vivo expression of NF1707 reduced NF protein levels in BLA projection neurons more effectively. Thus, we focused our main analysis on physiological and behavioral effects on NF1707, while results from NF1196 are given in the Supplementary Information, and provide an important comparison.
Neurofascin Knockdown in BLA Alters mIPSCs
To evaluate the impact of NF knockdown on synaptic transmission, mIPSCs (Figure 3a) were measured in brain slices from lentivirus-infected rats. Comparison of EGFP-positive, NF1707 (n=28) and CTR (n=25)-infected neurons revealed that knockdown of NF resulted in a significant leftward shift in the amplitude distribution (CTR vs NF1707, for amplitude, P<0.001, K–S test; Figure 3b) and a reduction in the average mIPSC amplitude (t-test, t(51)=2.163, P<0.01; Figure 3d). Conversely, the distribution of inter-event intervals was unaltered (CTR vs NF1707, for inter-event interval, P>0.05, K–S test; Figure 3c) and the average mIPSC frequency were unaltered (t(51)=0.329, P=0.743; Figure 3e). Together, this suggests a functional postsynaptic reduction of GABAergic synaptic transmission by NF knockdown in BLA projection neurons.
Neurofascin Knockdown Reduces Cellular Excitability in BLA
Next, we measured cellular excitability from EGFP-expressing neurons infected with CTR (n=14) or NF1707 (n=15) in BLA projection neurons. We injected a series of depolarizing current steps to assess the number of action potentials (Figure 4a–c). Repeated-measure ANOVA revealed that projection neurons fired significantly less after NF knockdown (group × current steps intensity interaction: F(1.973,53.279)=19.648, P<0.001, Greenhouse–Geisser correction) (Figure 4d). In addition, NF knockdown led to a significant hyperpolarization of the resting membrane potential (t(27)=4.192, P<0.001; Supplementary Figure S5a), while the action potential threshold was significantly depolarized (t(27)=3.820, P<0.01; Supplementary Figure S5b). As a result, the threshold current was significantly increased in NF1707-infected neurons (t(27)=4.487, P<0.001; Supplementary Figure S5c), while the input resistance was unaltered (t(27)=0.036, P=0.971; Supplementary Figure S5d). Moreover, the action potential half-width was significantly increased after NF knockdown (t(27)=2.735, P<0.05; Supplementary Figure S5f), while the amplitude was not altered (t(27)=1.591, P=0.123; Supplementary Figure S5e). Together, these experiments indicate that NF knockdown reduces firing patterns and compromises cellular excitability of BLA projection neurons.
Neurofascin Knockdown Impairs BLA Synaptic Plasticity
To address the functional physiological consequences of NF knockdown, we turned to in vivo recordings, and examined properties of vSub-evoked field potentials in the BLA in animals with confirmed viral expression and recording sites (Supplementary Figure S6). Comparing NF1707 (n=10) and CTR (n=8) groups, no significant effect on basal excitability, as assessed by input–output curves, was found despite a trend toward increased excitability (Supplementary Figure S7; F(1,16)=2.280, P=0.151). Repeated-measure ANOVA showed no group × intensity interaction (F(2.080,33.27)=1.984, P=0.152, Greenhouse–Geisser correction). Upon application of HFS, vSub-evoked field potentials in the BLA were effectively potentiated in the control group, but not in the NF knockdown group as revealed by mixed model repeated-measure ANOVA (group effect: (F(1,16)=16.27, P=0.001; and group × post-HFS time point interaction: F(2.071,33.136)=9.231, P=0.001, Greenhouse–Geisser correction; Figure 4f). The comparison of average potentiation across time points after induction also revealed a highly significant difference between the two groups (Figure 4g, t(16)=4.03, P=0.001). NF knockdown with the less effective NF1196 vector also reduced vSub-BLA LTP (Supplementary Figure S8b and c) without altering the input–output curve (Supplementary Figure S8a). Together, this demonstrates a specific impairment of BLA synaptic plasticity to vSub inputs after NF knockdown.
Neurofascin Knockdown Does Not Alter Acquisition and Expression of Cued Fear
To assess the impact on fear behavior, we first examined whether NF knockdown affects acquisition of conditioned fear. Animals expressing NF1707 or CTR (n=13 per group; see Supplementary Figure S9 for verification of virus expression) both displayed low pre-conditioning basal freezing levels in context A (t(24)=0.152, P=0.881; Figure 5a). During the training phase, freezing increased over the three conditioned stimulus (CS)–US pairings in both groups (Figure 5b) as assessed by repeated-measure ANOVA (effect of tones: F(2,48)=214.9, P=0.001; no group effect: F(1,24)=0.146, P=0.706; no group × tone interaction: F(2,48)=1.99, P=0.14) and remained at comparable levels in 2 min post training (t(24)=0.171, P=0.866; Figure 5c). Furthermore, animals in both groups retrieved the acquired fear and showed similar levels of freezing when tested the next day (Figure 5d, first five CS presentations at the beginning of extinction training t(24)=−1.028, P=0.321). Also, the less effective NF1196 did not alter baseline freezing, fear acquisition or fear expression (Supplementary Figure S10a–d). Together, this shows that NF knockdown in the BLA did not alter fear learning and memory.
Neurofascin Knockdown Impairs Extinction of Conditioned Fear
Twenty-four hours after fear conditioning, we administered a 3-day extinction training protocol in context B, where animals were exposed to five blocks of two CS each day (Figure 5d). While no differences in baseline freezing at the start of extinction training were apparent (Supplementary Figure S11), a mixed model repeated-measure ANOVA revealed significant differences in freezing over extinction blocks between the NF1707 and CTR groups (F(10.63,255.16)=12.13, P<0.001) with a significant block × group interaction (F(10.63,255.16)=3.44, P<0.001, Huynh–Feldt correction), but no general group effect (F(1,24)=3.12, P=0.090). Post hoc comparisons revealed that a significant group difference emerged on day 2 of extinction. The within-session analysis revealed a significant difference between the groups only on day 3 of extinction (main group effect: F(1,24)=19.145, P=0.001; no block × group interaction: F(4,96)=0.276, P=0893, Huynh–Feldt correction), but not on day 1 (group: F(1,24)=0.099, P=0.755; block × group interaction: F(3.62, 86.81)=0.786, P=0.526, Huynh–Feldt correction) or day 2 (group: F(1,24)=1.489, P=0.234; block × group interaction: F(3.64, 87.36)=2.086, P=0.096, Huynh–Feldt correction). The between-session analysis (comparing the last block with the first block of the session on the following day) did not reveal any group differences from extinction day 1 to day 2 (group: F(1,24)=0.094, P=0.761; block × group interaction: F(1,24)=0.483, P=0.494, Huynh–Feldt correction) or from day 2 to day 3 (group: F(1,24)=3.689, P=0.067; block × group interaction: F(1,24)=1.126, P=0.299, Huynh–Feldt correction). However, further analysis of average freezing in the initial phase (first five CSs on day 1) vs the late phase (last five CSs on day 3) of extinction confirmed that NF knockdown impaired cued fear extinction (first five CSs: t(24)=−1.028, P=0.321; last five CSs: t(24)=5.02, P<0.001). Accordingly, the overall reduction of difference in freezing percentage (first five CSs−last five CSs) was significantly reduced in the NF1707 vs the CTR group (Figure 5e; t(24)=4.31, P<0.001). With NF1196, a similar trend toward decreased extinction was observed (Supplementary Figure S10d and e). In summary, these data suggest that knockdown of NF impaired long-term memory of extinction for cued-conditioned fear.
Neurofascin Knockdown Does Not Affect Locomotor Activity and Anxiety-like Behavior
Finally, to rule out the possibility that the behavioral analysis of fear and extinction learning was confounded by changes in activity or anxiety-related behavior, we analyzed the rats’ behavior in the OFT and EPM test before fear conditioning. Neurofascin knockdown by NF1707 did not affect activity and anxiety-like behavior, as assessed by total distance moved (Supplementary Figure S12a; t(24)=0.99, P>0.05) or by percentage of distance moved in the center of the open-field arena (Supplementary Figure S12b; t(24)=0.57, P>0.05). Likewise, neither the total distance moved in the EPM arena (Supplementary Figure S12c; t(24)=1.61, P>0.05) nor the percentage of distance or the duration moved in the open arms showed any significant differences between groups (Supplementary Figure S12d and e; P>0.05). Similarly, NF1196 had no significant effect on anxiety-like behavior in all of these behavioral assays (Supplementary Figure S13). Together, these data strengthen the notion that knockdown of NF specifically impaired fear extinction memory without affecting anxiety-related behavior.
In the present study we investigated the role of AIS GABAergic synapses in BLA projection neurons in fear behavior by knocking down the neural cell adhesion molecule NF, which is associated with the stabilization of these synapses (Burkarth et al, 2007; Kriebel et al, 2011). In the hippocampus, NF controls the assembly of GABAergic synapses at the AIS (Kriebel et al, 2011). A recent study from our lab demonstrated that loss of these synapses in dorsal DG impacts local network activity and behavior, selectively increasing feedback inhibition and impairing hippocampal-dependent aversive learning in the two-way shuttle paradigm (Zitman et al, 2014). Here we demonstrate that a specific knockdown of NF in the BLA (1) exclusively reduced a pool of GABAergic synapses innervating the AIS of BLA projection neurons, resulting functionally in reduced GABAergic transmission and changes in excitability; (2) induced a deficit in LTP in the vSub–BLA pathway in vivo; and (3) impaired extinction while acquisition and expression of cued fear memory was intact, and anxiety-like behavior were unaltered. Together, this suggests that the manipulation of NF, resulting in a selective removal of AIS inhibitory synapses at BLA projection neurons, leads to a specific impairment of fear memory extinction.
Both NF-specific miRNAs were highly efficient in reducing NF mRNA in primary culture. In vivo reduction of NF protein expression was moderate for NF1707 and undetectable for NF1196. The discrepancy may be explained by the limited sensitivity of immunohistological analyses, or alternatively, by the stability of the NF protein. In the cerebellum, reduction of NF protein levels was observed in a tamoxifen-induced conditional mouse model only after 6 weeks while a synaptic phenotype was observed only after 16 weeks (Zonta et al, 2011). Effects of NF1196 were observed by in vivo electrophysiology performed more than 3 weeks after virus injection, while we were unable to detect effects on fear memory extinction after 2 weeks. Therefore, it is possible that a fully effective manipulation of NF levels with the NF1196 constructs is time-dependent. However, we observed synaptic alterations 2 weeks after lentiviral infection with NF1707 without overt alterations of the AIS based on AnkG analysis. The synaptic effects are well in line with earlier hippocampal studies (Kriebel et al, 2011; Zitman et al, 2014) showing a relatively rapid reduction of GABAergic synapses at the AIS, while perisomatic synapses were unaffected. Both NF knockdown constructs reduced GABAergic terminals at the AIS, but to a different extend, which is in line with their differential effect on extinction learning. Together, these findings indicate that our manipulation was specific and rules out off-target effects.
Because the AIS is exclusively targeted by AACs (Howard et al, 2005; Woodruff et al, 2010), NF knockdown likely perturbed the signaling of this specific set of interneurons onto BLA projection neurons. This notion was further supported by the observed reduction of mIPSC amplitude after NF knockdown. Usually, a change in the amplitude of mIPSCs reflects alterations in postsynaptic GABAA receptors while a change in the frequency of mIPSCs implicates an altered probability of transmitter release and/or changes in the number of active synapses (De Koninck and Mody, 1994; Otis et al, 1994). The reduction in average amplitude after NF knockdown may result from a decreased frequency of larger events, probably due to a reduction of number of postsynaptic GABAA receptors (Otis et al, 1994). However, the lack of observed alterations in the frequency could be explained by the fact that relative to the overall GABAergic input, only a very small number of synapses were completely removed at the AIS, while smaller events remained. Nevertheless, our results provide direct evidence that NF knockdown impairs GABAergic transmission within the BLA by reducing functional GABAA receptors at the postsynaptic site.
Moreover, due to its restriction to the AIS, knockdown of NF and the associated reduction in inhibitory inputs may also affect the properties of evoked action potentials. After NF knockdown, the firing rate was significantly reduced and the action potential half-width was increased, while the input resistance was unaltered. The reduced firing rate of neurons could be explained by their hyperpolarized resting membrane potential and a depolarized threshold potential, in accordance with Zonta et al (2011). Indeed, it has been demonstrated that AACs can control action potential generation at the AIS of projection neurons via their GABAergic inputs, either through changing the action potential threshold, or by shifting the site of origin (Douglas and Martin, 1990; Somogyi et al, 1982; Wefelmeyer et al, 2015; Veres et al, 2014). Accordingly, similar differences in action potential properties as seen after NF knockdown arise by shifting the action potential initiation site to the first node of Ranvier (Clark et al, 2005). Moreover, the reduced firing rate of projection neurons could also contribute to the observed LTP impairment (Cohen et al, 1999).
To investigate BLA-LTP in vivo, we chose the vSub–BLA pathway, which represents the major connection between hippocampus and BLA (Horovitz and Richter-Levin, 2015; Kim et al, 2013; Maren et al, 1995) and is known to be part of the fear conditioning and extinction circuitry (Biedenkapp and Rudy, 2009; Hobin et al, 2006). While impaired LTP in the vSub–BLA pathway after NF knockdown in the BLA may result from reduced neural excitability, its relation to reduced GABAergic inhibition by NF knockdown appears counterintuitive on first sight. However, it may be related to the fact that previous studies suggested rather excitatory net effects of AACs onto BLA projection neurons (Woodruff et al, 2010). Monosynaptic vSub–BLA projections exist (Mello et al, 1992), and can recruit projection neurons, but also local interneurons via feed-forward circuits, but the role of AACs remains unclear (Bazelot et al, 2015; Hübner et al, 2014). However, vSub afferents represent only a minor part of BLA afferents (for example, Canteras et al, 1992) and AACs are also reciprocally connected to projections neurons, suggesting they could also be part of local feedback circuits (Veres et al, 2014). Accordingly, vSub–BLA plasticity may be also shaped by intra-amygdalar local inhibitory networks. Cumulative evidence suggests that different BLA afferents target specific subpopulations of interneurons (Leitermann et al, 2016; McDonald et al, 2011; Unal et al, 2014), thus enabling input-specific modulation of BLA local networks, which in turn may affect induction of LTP in this structure. Notably, the input–output curve revealed a trend for increased BLA excitability in the NF1707 knockdown group, which could reflect local network changes that may affect further potentiation. As all animals received fear conditioning and extinction training before the BLA-LTP recordings in vivo, baseline excitability and plasticity could have been affected by this experience in interaction with the NF knockdown effects. Nevertheless, this was not observed for the NF1196 construct, while LTP was similarly reduced. This strongly suggests that impaired LTP was not a result of a priori increased excitability.
Within the extinction network, activity and plasticity of the BLA is considered critical for expression of fear, as well as the initial acquisition of extinction memory (Herry et al, 2008; Quirk and Mueller, 2008). Knockdown of NF in the BLA did not affect initial cued fear memory acquisition and consolidation, nor did it affect basal levels of anxiety-related behaviors, suggesting a specific extinction deficit (Dalton et al, 2008). This idea is in line with the role of NF in GABAergic transmission. Several studies converge on showing that extinction training globally increases GABAergic markers including gephyrin and GABAA receptor subunits in the BLA (Chhatwal et al, 2005; Heldt and Ressler, 2007; Lin et al, 2009), and interfering with GABAA receptor insertion by a TAT-conjugated peptide within BLA synapses impaired fear extinction (Lin et al, 2009). However, the role of specific interneuron subtype inputs remains less well understood. To the best of our knowledge, only one study addressed the role of such specific changes, demonstrating remodeling of perisomatic synapses in contextual fear extinction (Trouche et al, 2013). Knockdown of NF reduced GABAergic markers at the AIS, supporting the view that increased GABAergic transmission at this compartment is also required for extinction learning.
Disrupting the structural integrity of the AIS, for example, in Angelman syndrome due to increased expression of AnkG and the Na/K ATPase subunit α1, can indeed lead to impaired contextual fear memory (Kaphzan et al, 2013), but the role of specific synapses at this compartment remained elusive. The only interneuron subtype presently described that innervates the AIS are AACs or chandelier cells (Bazelot et al, 2015), and their inputs to the AIS were selectively targeted by NF knockdown in our study. The resulting impairment in extinction of auditory-cued fear memory adds new insights into the role of such AAC inputs in behavior, even if the integrity of the AIS structure itself is intact.
This model of NF knockdown adds to our understanding of the mechanisms by which specific local GABAergic interneurons contribute to extinction of fear memory. Considering the importance of extinction deficits as a hallmark of anxiety disorders such as phobias and post-traumatic stress disorder, a better understanding of region-specific modification of GABAergic synapses may provide novel and more specific targets for therapeutic intervention.
Funding and disclosure
This research was supported by the Werner Reichardt Centre for Integrative Neuroscience (CIN) at the University of Tuebingen, an Excellence Cluster funded by the Deutsche Forschungsgemeinschaft (DFG) within the framework of the Excellence Initiative (EXC 307, Pool Project 2012-09 to IE, HJV and GRL), and by funds from the Charitable Hertie Foundation (IE). The authors declare no conflict of interest.
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We are thankful to Elhanan Ben Yishay for his technical assistance.
Supplementary Information accompanies the paper on the Neuropsychopharmacology website
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Saha, R., Knapp, S., Chakraborty, D. et al. GABAergic Synapses at the Axon Initial Segment of Basolateral Amygdala Projection Neurons Modulate Fear Extinction. Neuropsychopharmacol 42, 473–484 (2017). https://doi.org/10.1038/npp.2016.205
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