Abstract
High-speed atomic force microscopy (HS-AFM) can be used to visualize function-related conformational changes of single soluble proteins. Similar studies of single membrane proteins are, however, hampered by a lack of suitable flat, non-interacting membrane supports and by high protein mobility. Here we show that streptavidin crystals grown on mica-supported lipid bilayers can be used as porous supports for membranes containing biotinylated lipids. Using SecYEG (protein translocation channel) and GlpF (aquaglyceroporin), we demonstrate that the platform can be used to tune the lateral mobility of transmembrane proteins to any value within the dynamic range accessible to HS-AFM imaging through glutaraldehyde-cross-linking of the streptavidin. This allows HS-AFM to study the conformation or docking of spatially confined proteins, which we illustrate by imaging GlpF at sub-molecular resolution and by observing the motor protein SecA binding to SecYEG.
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Acknowledgements
This work was supported by the Austrian Science Fund (FWF, P25844 to J.P.), the European Fund for Regional Development (EFRE, Regio 13) and the Federal State of Upper Austria. The authors thank H. Gruber for helpful discussion and Q. Beatty for editorial help.
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A.K. and J.P. performed HS-AFM experiments and performed data analysis. B.N., B.P., and E.K. performed fluorescence experiments and did data analysis. A.K., A.H., D.G.K., R.K., K.W., L.W., C.S., N.O, and J.P. developed sample preparation techniques. J.P. and A.K. designed the experiments. A.K., J.P. and P.P. prepared the final manuscript.
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Karner, A., Nimmervoll, B., Plochberger, B. et al. Tuning membrane protein mobility by confinement into nanodomains. Nature Nanotech 12, 260–266 (2017). https://doi.org/10.1038/nnano.2016.236
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DOI: https://doi.org/10.1038/nnano.2016.236
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