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Protein-nanoparticle interactions

What does the cell see?

Nature Nanotechnology volume 4, pages 546–547 (2009)Cite this article

Fluorescence correlation spectroscopy is used as a quantitative method to understand the binding and exchange behaviour of proteins on the surfaces of nanoparticles.

Surfaces of nanoparticles that are in contact with a biological milieu are in a dynamic exchange with biomolecules such as proteins1,2,3 and lipids. The identity and lifetime of these proteins3,4 on the particle surface can affect the way cells interact with, recognize and process the nanoparticles, and this has important implications for safety considerations5,6 and the practice of nanomedicine7. Quantitative methods are therefore needed to probe the binding dynamics and exchange kinetics of the proteins so that the biological impact of these adsorbed biomolecules can be understood.

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Figure 1: Nanoparticle–protein complexes as seen by the cell.

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Authors and Affiliations

  1. Iseult Lynch, Anna Salvati and Kenneth A. Dawson are in the Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland. iseult@fiachra.ucd.ie; kenneth@fiachra.ucd.ie,

    Iseult Lynch, Anna Salvati & Kenneth A. Dawson

  2. kenneth@fiachra.ucd.ie,

    Iseult Lynch, Anna Salvati & Kenneth A. Dawson

Authors
  1. Iseult Lynch
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  2. Anna Salvati
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  3. Kenneth A. Dawson
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Lynch, I., Salvati, A. & Dawson, K. What does the cell see?. Nature Nanotech 4, 546–547 (2009). https://doi.org/10.1038/nnano.2009.248

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