Abstract
Activation by parathyroid hormone (PTH)1 and peripheral distribution2,3 the of PTH2 receptor (PTH2-R) are well described. Highest expression of PTH2-R is found in the brain1, where PTH synthesis is undetectable. We have now purified and sequenced a new polypeptide from bovine hypothalamus (referred to here as tuberoinfundibular peptide of 39 residues; TIP39), a potent and selective agonist at the PTH2-R. The PTH2-R was highly expressed in hypothalamic areas that regulate pituitary hormone secretion and spinal cord regions involved in pain perception.
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Acknowledgements
Large-scale tissue extraction was performed by Thomas McCloud and Andrew Burnette of the National Cancer Institute Natural Products Support Group and microsequencing by the Harvard University Microchemistry Facility. TIP39 was synthesized by AnaSpec, Inc. F-11 and N18TG2 cells were obtained from H. Gainer. We thank Chris Evans, Lisa Baptiste, Ed Unsworth and Howard Jaffe for advice and assistance and Mike Brownstein and Hal Gainer for reviewing the manuscript.
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Usdin, T., Hoare, S., Wang, T. et al. TIP39: a new neuropeptide and PTH2-receptor agonist from hypothalamus. Nat Neurosci 2, 941–943 (1999). https://doi.org/10.1038/14724
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DOI: https://doi.org/10.1038/14724
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