Naturally occurring rearrangements of synaptic terminals are common in the nervous systems of young mammals, but little is known about their incidence in adults. Using transgenic mice that express yellow fluorescent protein (YFP) in axons, we repeatedly imaged nerve terminals in the parasympathetic submandibular ganglion. We found that the pattern of synaptic branches underwent significant rearrangements over several weeks in young adult mice. In older mice, rearrangements were less common, and synaptic patterns on individual neurons were recognizable for many months to years. Axonal branches frequently retracted or extended on a time scale of minutes in young adult mice, but seldom in mature animals. These results provide direct evidence for a decrease in plasticity of interneuronal connections as animals make the transition from young adulthood to middle age. The long-term stability of synaptic patterns could provide a structural basis for the persistence of memory in the adult nervous system.
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We thank H. Suk-Woo for help with data analysis. This work was supported by grants from the US National Institutes of Health (NIH) to J.W.L. and J.R.S. W-B.G. and E.K. were supported by grants to W-B.G. from the NIH (R01 NS41846-01) and the Ellison Foundation. Support from the Bakewell NeuroImaging Fund is gratefully acknowledged.
The authors declare no competing financial interests.
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Gan, WB., Kwon, E., Feng, G. et al. Synaptic dynamism measured over minutes to months: age-dependent decline in an autonomic ganglion. Nat Neurosci 6, 956–960 (2003). https://doi.org/10.1038/nn1115
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