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PDZ binding of TARPγ-8 controls synaptic transmission but not synaptic plasticity

Nature Neuroscience volume 14, pages 1410–1412 (2011)Cite this article

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Abstract

The reduction in synaptic transmission and plasticity in mice lacking the hippocampus-enriched AMPA receptor (AMPAR) auxiliary subunit TARPγ-8 could be a result of a reduction in AMPAR expression or of the direct action of γ-8. We generated TARPγ-8Δ4 knock-in mice lacking the C-terminal PDZ ligand. We found that synaptic transmission and AMPARs were reduced in the mutant mice, but extrasynaptic AMPAR expression and long-term potentiation (LTP) were unaltered. Our findings suggest that there are distinct TARP-dependent mechanisms for synaptic transmission and LTP.

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Figure 1: TARPγ-8 PDZ binding is necessary for synaptic localization of AMPARs.
Figure 2: The γ-8 PDZ ligand modulates AMPAR-mediated basal transmission, but not LTP.

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Acknowledgements

We thank R. Nicoll (University of California, San Francisco) for providing the γ-8−/− mouse. The monoclonal antibody to SynDIG1 was developed by and/or obtained from the UC Davis/US National Institutes of Health NeuroMab. This work is supported by grants from the National Institute of Mental Health (MH077939 to S.T.) and from the US National Institutes of Health (NS050570 and NS065251 to J.A.K.).

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Author notes

  1. Akio Sumioka and Travis E Brown: These authors contributed equally to this work.

Authors and Affiliations

  1. Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut, USA

    Akio Sumioka & Susumu Tomita

  2. Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA

    Akio Sumioka & Susumu Tomita

  3. Depatment of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island, USA

    Travis E Brown & Julie A Kauer

  4. Department of Neuroscience, Brown University, Providence, Rhode Island, USA

    Travis E Brown & Julie A Kauer

  5. Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana, USA

    Akihiko S Kato & David S Bredt

Authors
  1. Akio Sumioka
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  2. Travis E Brown
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  3. Akihiko S Kato
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  4. David S Bredt
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  5. Julie A Kauer
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  6. Susumu Tomita
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Contributions

S.T. and J.A.K. conceived the project and wrote the manuscript. A.S., T.E.B., A.S.K. D.S.B. and S.T. performed all of the experiments and analyzed the results. All of the authors contributed to the final version of the manuscript.

Corresponding authors

Correspondence to Julie A Kauer or Susumu Tomita.

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The authors declare no competing financial interests.

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Supplementary Figures 1–7 and Supplementary Methods (PDF 12573 kb)

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Sumioka, A., Brown, T., Kato, A. et al. PDZ binding of TARPγ-8 controls synaptic transmission but not synaptic plasticity. Nat Neurosci 14, 1410–1412 (2011). https://doi.org/10.1038/nn.2952

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