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Software for bead-based registration of selective plane illumination microscopy data

Nature Methods volume 7, pages 418419 (2010) | Download Citation

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Acknowledgements

We thank Carl Zeiss Microimaging for access to the SPIM demonstrator; R.K. Ejsmont (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden) for His-YFP flies; D. White, E. Dimitrova, M. Sarov and P. Campinho for help with imaging and B. Schmid for help with 3D viewer programming. S.P. and S.S. were supported by a Dresden International Graduate School for Biomedicine and Bioengineering doctorate stipend. J.S. and P.T. acknowledge funding from the Human Frontier Science Program Research grant RGY0084.

Author information

Author notes

    • Stephan Preibisch
    •  & Stephan Saalfeld

    These authors contributed equally to this work.

Affiliations

  1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

    • Stephan Preibisch
    • , Stephan Saalfeld
    • , Johannes Schindelin
    •  & Pavel Tomancak

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Pavel Tomancak.

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–8, Supplementary Table 1, Supplementary Methods, Supplementary Data

Zip files

  1. 1.

    Supplementary Software

    SPIM registration plugin for Fiji.

Videos

  1. 1.

    Supplementary Video 1

    Rotation capabilities of the selective plane illumination microscope.

  2. 2.

    Supplementary Video 2

    Visualization of the global optimization of eight view selective plane illumination microscope acquisition of C. elegans with 40×, 0.8 objective.

  3. 3.

    Supplementary Video 3

    Visualization of the global optimization of tiled single-photon confocal acquisition of Drosophila with 40×, 0.8 objective.

  4. 4.

    Supplementary Video 4

    Three-dimensional rendering of Drosophila gastrulation time-lapse acquired from seven angles.

  5. 5.

    Supplementary Video 5

    Three-dimensional rendering of Drosophila embryogenesis time-lapse acquired from five angles.

  6. 6.

    Supplementary Video 6

    Three-dimensional rendering of fixed Drosophila reconstructed from multiview spinning disc acquisition.

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DOI

https://doi.org/10.1038/nmeth0610-418

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