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References
Kirkpatrick, D.S., Denison, C. & Gygi, S.P. Nat. Cell Biol. 7, 750–757 (2005).
Shevchenko, A. et al. Anal. Chem. 68, 850–858 (1996).
Boja, E.S. & Fales, H.M. Anal. Chem. 73, 3576–3582 (2001).
Ong, S.E. et al. Mol. Cell. Proteomics 1, 376–386 (2002).
Dahl, K.H. & McKinley-McKee, J.S. Bioorg. Chem. 10, 329–341 (1981).
Zhang, Y. Genes Dev. 17, 2733–2740 (2003).
Acknowledgements
M.L.N. is a recipient of a European Molecular Biology Organization fellowship. M.V. is supported by a fellowship of the Dutch Cancer Society. This work was partly supported by High-throughput Epigenetic Regulatory Organization in Chromatin (HEROIC) (LSHG-CT-2005-018883).
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Nielsen, M., Vermeulen, M., Bonaldi, T. et al. Iodoacetamide-induced artifact mimics ubiquitination in mass spectrometry. Nat Methods 5, 459–460 (2008). https://doi.org/10.1038/nmeth0608-459
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DOI: https://doi.org/10.1038/nmeth0608-459
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