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Temporally resolved SMLM (with large PAR shift) enabled visualization of dynamic HA cluster formation and migration in a live cell
Scientific Reports Open Access 02 August 2023
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Acknowledgements
We thank J. Enninga for assistance with experiments, and T. Duong and R. Fesce for helpful insight in the preparation of the manuscript.
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Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–2, Supplementary Methods, Supplementary Note 1, Supplementary Discussion (PDF 903 kb)
Supplementary Software 1
QuickPALM software (ZIP 5714 kb)
Supplementary Software 2
Laser control software (ZIP 5596 kb)
Supplementary Movie 1
A 3D presentation of microtubules shown in Figure 1e,f. The viewing angle is rotated here to show different perspectives. Scale bar, 1,000 nm. (MOV 1376 kb)
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Henriques, R., Lelek, M., Fornasiero, E. et al. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7, 339–340 (2010). https://doi.org/10.1038/nmeth0510-339
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DOI: https://doi.org/10.1038/nmeth0510-339
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