QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

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Figure 1: Three-dimensional dSTORM imaging of β-III-tubulin–labeled microtubules in neuronal cells.


  1. 1

    Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    CAS  Article  Google Scholar 

  2. 2

    Hess, S.T., Girirajan, T.P. & Mason, M.D. Biophys. J. 91, 4258–4272 (2006).

    CAS  Article  Google Scholar 

  3. 3

    Betzig, E. et al. Science 313, 1642–1645 (2006).

    CAS  Article  Google Scholar 

  4. 4

    Huang, B., Jones, S.A., Brandenburg, B. & Zhuang, X. Nat. Methods 5, 1047–1052 (2008).

    CAS  Article  Google Scholar 

  5. 5

    Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).

    CAS  Article  Google Scholar 

  6. 6

    Wolter, S. et al. J. Microsc. 237, 12–22 (2010).

    CAS  Article  Google Scholar 

  7. 7

    Hedde, P.N., Fuchs, J., Oswald, F., Wiedenmann, J. & Nienhaus, G. U. Nat. Methods 6, 689–690 (2009).

    CAS  Article  Google Scholar 

  8. 8

    Högbom, J.A. Astron. Astrophys. 15 (Suppl.), 417–426 (1974).

    Google Scholar 

  9. 9

    Stuurman, N., Amodaj, N. & Vale, R.D. Microscopy Today 15, 42–43 (2007).

    Article  Google Scholar 

  10. 10

    Heilemann, M. et al. Angew. Chem. Int. Edn Engl. 47, 617–6176 (2008).

    Article  Google Scholar 

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We thank J. Enninga for assistance with experiments, and T. Duong and R. Fesce for helpful insight in the preparation of the manuscript.

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Correspondence to Ricardo Henriques.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–2, Supplementary Methods, Supplementary Note 1, Supplementary Discussion (PDF 903 kb)

Supplementary Software 1

QuickPALM software (ZIP 5714 kb)

Supplementary Software 2

Laser control software (ZIP 5596 kb)

Supplementary Movie 1

A 3D presentation of microtubules shown in Figure 1e,f. The viewing angle is rotated here to show different perspectives. Scale bar, 1,000 nm. (MOV 1376 kb)

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Henriques, R., Lelek, M., Fornasiero, E. et al. QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ. Nat Methods 7, 339–340 (2010). https://doi.org/10.1038/nmeth0510-339

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