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Computational correction of index switching in multiplexed sequencing libraries

Nature Methods volume 15, pages 305307 (2018) | Download Citation

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  1. 1.

    et al. Cell Metab. 24, 593–607 (2016).

  2. 2.

    et al. Science 357, 661–667 (2017).

  3. 3.

    Illumina Inc. Effects of Index Misassignment on Multiplexing and Downstream Analysis. Publication No. 770-2017-004-C QB # 5420 (Illumina Inc., 2017).

  4. 4.

    et al. bioRxiv preprint at (2017).

  5. 5.

    , , , & bioRxiv preprint at (2018).

  6. 6.

    et al. bioRxiv preprint at (2017).

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This work was supported by the Swedish Research Council (grant 2017-01062 to R. Sandberg), the Bert L. and N. Kuggie Vallee Foundation (to R. Sandberg), a National Science Foundation Graduate Research Fellowship (grant DGE 1147470 to G.S.), the NIH (grant R01CA86065 to I.L.W.), the California Institute for Regenerative Medicine (grant GC1R-06673-A), to M. Snyder; Collaborative Reserch Project to I.L.W. and the Virginia and D.K. Ludwig Fund for Cancer Research (to I.L.W.)

Author information


  1. Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

    • Anton J M Larsson
    •  & Rickard Sandberg
  2. Department of Bioengineering, Stanford University School of Engineering, Stanford, California, USA.

    • Geoff Stanley
  3. Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA.

    • Rahul Sinha
    •  & Irving L Weissman


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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Rickard Sandberg.

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    unspread software for index switching correction

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