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Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo

Nature Methods volume 15, pages 238239 (2018) | Download Citation

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This work was supported by the National Science Foundation (DGE1144152 to C.A.L.), National Institutes of Health (R35 GM118158 to J.K.J. and R00 HG008399 to L.P.), the Defense Advanced Research Projects Agency (HR0011-17-2-0042 to J.K.J. and L.P.), and the Desmond and Ann Heathwood Massachusetts General Hospital Research Scholar Award (J.K.J.). We thank B. Kleinstiver, J. Lopez, and A. Anthony for helpful discussions.

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Author notes

    • Caleb A Lareau
    • , Kendell Clement
    •  & Jonathan Y Hsu

    These authors contributed equally to this work.


  1. Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

    • Caleb A Lareau
    • , Kendell Clement
    • , Jonathan Y Hsu
    • , Vikram Pattanayak
    • , J Keith Joung
    • , Martin J Aryee
    •  & Luca Pinello
  2. Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.

    • Caleb A Lareau
    •  & Martin J Aryee
  3. Cell Circuits and Epigenomics Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

    • Caleb A Lareau
    • , Kendell Clement
    • , Martin J Aryee
    •  & Luca Pinello
  4. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA.

    • Kendell Clement
    • , Vikram Pattanayak
    • , J Keith Joung
    • , Martin J Aryee
    •  & Luca Pinello
  5. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.

    • Kendell Clement
  6. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Jonathan Y Hsu


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Competing interests

J.K.J. has financial interests in Beam Therapeutics, Editas Medicine, Monitor Biotechnologies, Pairwise Plants, Poseida Therapeutics, and Transposagen Biopharmaceuticals. M.J.A. has financial interests in Monitor Biotechnologies. J.K.J.'s and M.J.A.'s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.

Corresponding authors

Correspondence to J Keith Joung or Martin J Aryee or Luca Pinello.

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