Mammalian genomes are spatially highly organized, a feature that has a role in many aspects of gene expression and cell biology. The cellular factors involved in regulating and maintaining genome structure have historically been challenging to identify owing to a lack of high-throughput screening methods. To address this issue, Shachar et al. developed high-throughput imaging position mapping (HIPMap), in which the location of target genes in the nucleus is monitored using fluorescence in situ hybridization (FISH). HIPMap applies fully automated FISH, imaging and data analysis in a 384-well format. The authors used the procedure to screen an siRNA library for factors that led to changes in the positions of the targeted genes. Their screen revealed more than fifty factors involved in genome organization, most of which were not previously identified.
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High-throughput discovery of genome organizers. Nat Methods 12, 909 (2015). https://doi.org/10.1038/nmeth.3598
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DOI: https://doi.org/10.1038/nmeth.3598
