Light-sheet fluorescence microscopy for quantitative biology

In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.

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Figure 1: Basic optical arrangement in LSFM.
Figure 2: LSFM imaging of a live Tribolium castaneum embryo expressing nuclear GFP.

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Acknowledgements

E.H.K.S.'s research is funded through CEF-MC (EXC 115) by the Deutsche Forschungsgemeinschaft.

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Correspondence to Ernst H K Stelzer.

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E.H.K.S. is an inventor or co-inventor of two relevant patents owned by the European Molecular Biology Laboratory Enterprise Management Technology Transfer GmbH: (i) microscope with a viewing direction perpendicular to the illumination direction, US 7554725; DE 10257423 and (ii) single plane illumination microscope US 20070109633 A1; PCT/EP03/05991.

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Stelzer, E. Light-sheet fluorescence microscopy for quantitative biology. Nat Methods 12, 23–26 (2015). https://doi.org/10.1038/nmeth.3219

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