Commentary | Published:

Light-sheet imaging for systems neuroscience

Nature Methods volume 12, pages 2729 (2015) | Download Citation

Developments in electrical and optical recording technology are scaling up the size of neuronal populations that can be monitored simultaneously. Light-sheet imaging is rapidly gaining traction as a method for optically interrogating activity in large networks and presents both opportunities and challenges for understanding circuit function.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    & Ann. Phys. 315, 1–39 (1902).

  2. 2.

    Award ceremony speech. Nobelprize.org (1926).

  3. 3.

    , & J. Microsc. 170, 229–236 (1993).

  4. 4.

    , , & Opt. Express 10, 145–154 (2002).

  5. 5.

    , , , & Science 305, 1007–1009 (2004).

  6. 6.

    , & Neuron 57, 661–672 (2008).

  7. 7.

    , , , & Nat. Methods 10, 413–420 (2013).

  8. 8.

    et al. Front. Neural Circuits 7, 65 (2013).

  9. 9.

    et al. Nat. Methods 11, 883–884 (2014).

  10. 10.

    , , & Nat. Neurosci. 17, 953–961 (2014).

  11. 11.

    , , & Nat. Methods 9, 755–763 (2012).

  12. 12.

    , , , & Nat. Methods 9, 730–733 (2012).

  13. 13.

    , , , & Nat. Methods 8, 757–760 (2011).

  14. 14.

    & Nat. Neurosci. 17, 1500–1509 (2014).

  15. 15.

    & Nat. Rev. Neurosci. 8, 700–711 (2007).

  16. 16.

    et al. Nature 454, 995–999 (2008).

  17. 17.

    et al. Nat. Methods 11, 941–950 (2014).

  18. 18.

    , & Neuron 83, 1249–1252 (2014).

Download references

Author information

Affiliations

  1. Philipp J. Keller, Misha B. Ahrens and Jeremy Freeman are at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

    • Philipp J Keller
    • , Misha B Ahrens
    •  & Jeremy Freeman

Authors

  1. Search for Philipp J Keller in:

  2. Search for Misha B Ahrens in:

  3. Search for Jeremy Freeman in:

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Philipp J Keller or Misha B Ahrens or Jeremy Freeman.

About this article

Publication history

Published

DOI

https://doi.org/10.1038/nmeth.3214

Further reading

Newsletter Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing