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A reprogrammable mouse strain from gene-targeted embryonic stem cells

Abstract

The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.

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Figure 1: An ESC-based reprogramming system.
Figure 2: Derivation and characterization of reprogrammable mice.

References

  1. Takahashi, K. & Yamanaka, S. Cell 126, 663–676 (2006).

    Article  CAS  Google Scholar 

  2. Brambrink, T. et al. Cell Stem Cell 2, 151–159 (2008).

    Article  CAS  Google Scholar 

  3. Stadtfeld, M., Maherali, N., Breault, D.T. & Hochedlinger, K. Cell Stem Cell 2, 230–240 (2008).

    Article  CAS  Google Scholar 

  4. Maherali, N. et al. Cell Stem Cell 3, 340–345 (2008).

    Article  CAS  Google Scholar 

  5. Wernig, M. et al. Nat. Biotechnol. 26, 916–924 (2008).

    Article  CAS  Google Scholar 

  6. Hockemeyer, D. et al. Cell Stem Cell 3, 346–353 (2008).

    Article  CAS  Google Scholar 

  7. Sommer, C.A. et al. Stem Cells 27, 543–549 (2009).

    Article  CAS  Google Scholar 

  8. Beard, C., Hochedlinger, K., Plath, K., Wutz, A. & Jaenisch, R. Genesis 44, 23–28 (2006).

    Article  CAS  Google Scholar 

  9. Utikal, J. et al. Nature 460, 1145–1148 (2009).

    Article  CAS  Google Scholar 

  10. Mikkelsen, T.S. et al. Nature 454, 49–55 (2008).

    Article  CAS  Google Scholar 

  11. Sridharan, R. et al. Cell 136, 364–377 (2009).

    Article  CAS  Google Scholar 

  12. Lengner, C.J. et al. Cell Stem Cell 1, 403–415 (2007).

    Article  CAS  Google Scholar 

  13. Markoulaki, S. et al. Nat. Biotechnol. 27, 169–171 (2009).

    Article  CAS  Google Scholar 

  14. Blelloch, R., Venere, M., Yen, J. & Ramalho-Santos, M. Cell Stem Cell 1, 245–247 (2007).

    Article  CAS  Google Scholar 

  15. Eminli, S. et al. Nat. Genet. 41, 968–976 (2009).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank G. Mostoslavsky (Boston University School of Medicine) for the STEMCCA construct and secondary viral MEFs, A. Foudi and S. Eminli for help with hematopoietic cell isolation and culture, A. Khalil for help in cloning the Collagen-OKSM targeting construct, J. Polo for help with cell culture as well as for inspiring discussions, and C. Konrad for advice on tumor histology. M.S. was supported by a postdoctoral fellowship from the Schering Foundation, and K.H. was supported by a US National Institutes of Health Director's Innovator Award as well as by funds provided by the Harvard Stem Cell Institute and Massachusetts General Hospital.

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M.S. and K.H. conceived the study; N.M. performed blastocyst injections; M.S. performed all other experiments with help from M.B.; M.S. and K.H. analyzed the data and wrote the manuscript.

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Correspondence to Konrad Hochedlinger.

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Stadtfeld, M., Maherali, N., Borkent, M. et al. A reprogrammable mouse strain from gene-targeted embryonic stem cells. Nat Methods 7, 53–55 (2010). https://doi.org/10.1038/nmeth.1409

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