B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion

Abstract

The B7 family members B7-1 and B7-2 interact with CD28 and constitute an essential T-cell co-stimulatory pathway in the initiation of antigen-specific humoral and cell-mediated immune response. Here, we describe a third member of the B7 family, called B7-H1 that does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). Ligation of B7-H1 co-stimulated T-cell responses to polyclonal stimuli and allogeneic antigens, and preferentially stimulated the production of interleukin-10. Interleukin-2, although produced in small amounts, was required for the effect of B7-H1 co-stimulation. Our studies thus define a previously unknown co-stimulatory molecule that may be involved in the negative regulation of cell-mediated immune responses.

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Figure 1: Characterization and expression pattern of human B7-H1.
Figure 2: Binding of B7-H1 to CTLA4, ICOS and CD28.
Figure 3: Co-stimulation of T-cell growth by B7-H1Ig.
Figure 4: Cytokine secretion of resting T cells by B7-H1 co-stimulation.
Figure 5: B7-H1-co-stimulated T-cell proliferation and IL-10 production is IL-2-dependent.

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Acknowledgements

We thank J. Lau and K. Jensen for editing the manuscript. This work was supported in part by the Mayo Foundation and National Institutes of Health grant CA79915. G.Z. is supported by National Institutes of Health training grant CA09127. The accession number for the human B7-H1 sequence in the GenBank is AF177937.

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Correspondence to Lieping Chen.

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Dong, H., Zhu, G., Tamada, K. et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5, 1365–1369 (1999). https://doi.org/10.1038/70932

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