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Increased sensitivity of HIV-1 antibody detection

Nature Medicinevolume 3page1258 (1997) | Download Citation



Clinical trial results from 11,344 paired urine and serum samples revealed 1,181 HIV-1–positive individuals confirmed by western blot (WB). There were 25 discrepant samples: 10 were urine enzyme immunassay (EIA) and WB positive, serum non-reactive and serum WB negative or indeterminate, and 15 were serum EIA and WB positive, urine EIA non-reactive or urine WB negative or indeterminate. Serum samples, HIV-1 antibody WB confirmed, revealed a 99.15% sensitivity (1,171 out of 1,181); urine samples, HIV-1 antibody WB confirmed, showed a 98.73% sensitivity (1,166 out of 1,181). This study demonstrated that neither serum nor urine results alone are as sensitive for HIV-1 antibody detection as combined results of both samples.

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  1. 1

    Gottfried T.D. & Urnovitz H.B. HIV-1 Testing: Product Development Strategies. Trends in Biotechnology. 8, 35–40 (1990).

  2. 2

    Gallo D., George J.R., Fitchen, J.H., Goldstein, A.S. & Hindahl, M.S. Evaluation of a sytstem using oral mucosal transudate for HIV-1 antibody screening and confirmatory testing. JAMA 277, 254–8 (1997).

  3. 3

    Urnovitz, H.B., W.H., Murphy T.D., Gottfried & Friedman-Kien, A.E. Urine-Based Diagnostic Technologies. Trends in Biotechnology 14, 361–364 (1996).

  4. 4

    Hashida, S. et al. Shortening of the window period in diagnosis of HIV-1 infection by simultaneous detection of p24 antigen and antibody IgG to p17 and reverse transcriptase in serum with ultrasensitive enzyme immunoassay. J Virol Methods 62, 43–53 (1996).

  5. 5

    Charneau, P. et al. Isolation and envelope sequence of a highly divergent HIV-1 isolate: definition of a new HIV-1 group. Virology 205, 247–53 (1994).

  6. 6

    Urnovitz, H.B. et al. HIV-1 antibody serum negativity with urine positivity. Lancet 342, 1458–1459 (1993).

  7. 7

    Shearer, G.M. & Clerici, M. Protective immunity against HIV infection: has nature done the experiment for us? Immunol. Today 17, 21–24 (1996).

  8. 8

    Mazzoli, S. et al. HIV-Specific Mucosal and Cellular Immunity in HIV-Seronegative Partners of HIV-Seropositive Individuals. Nature Medicine, 1250 (in Press, 1997).

  9. 9

    Sordillo, E.M. et al. Western Blot Testing of HIV EIA Reactive Urine Samples. Am. Soc. Micro. Abstr. #V–70 (1997).

  10. 10

    Urnovitz, H.B. & Murphy, W.H., Human Retroviruses: Nature, Occurrence and Clinical Implications in Human Disease. Clin Micro Reviews 9, 72–99 (1996).

  11. 11

    CalypteTM HIV-1 Urine EIA Package Insert, Berkeley, CA., Calypte Biomedical. (1996)

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  1. Calypte Biomedical, 1440 Fourth Street, Berkeley, California, 94710, USA

    • Howard B. Urnovitz
    • , Jerrilyn C. Sturge
    •  & Toby D. Gottfried


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