NMR indicates that the small molecule RITA does not block p53-MDM2 binding in vitro

Article metrics

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: HSQC spectra of the 15N-labeled MDM2 (residues 1–118) with or without RITA.
Figure 2: HSQC spectra of the flexible N-terminal residues (1–93) of the 15N-labeled p53 (the residues of the p53 DNA core domain are mostly not seen because of their broad linewidths).

References

  1. 1

    Issaeva, N. et al. Nat. Med. 10, 1321–1328 (2004).

  2. 2

    Stockman, B.J. & Dalvit, C. Prog. NMR Spectrosc. 41, 187–231 (2002).

  3. 3

    Lepre, C.A., Moore, J.M. & Peng, J.W. Chem. Rev. 104, 3641–3675 (2004).

  4. 4

    Bell, S., Klein, C., Müller, L., Hansen, S. & Buchner, J. J. Mol. Biol. 322, 917–927 (2002).

  5. 5

    Ayed, A. et al. Nat. Struct. Biol. 8, 756–760 (2001).

  6. 6

    Lee, H. et al. J. Biol. Chem. 275, 29426–29432 (2000).

  7. 7

    Kussie, P.H. et al. Science 274, 948–953 (1996).

  8. 8

    Picksley, S.M., Vojtesek, B., Sparks, A. & Lane, D.P. Oncogene 9, 2523–2529 (1994).

  9. 9

    D'Silva, L. et al. J. am. Chem. Soc. 127, 13220–13226 (2005)

  10. 10

    Vassilev, L.T. et al. Science 303, 844–848 (2004).

Download references

Author information

Supplementary information

Supplementary Fig. 1

HSQC spectra of the 15N labeled MDM2 (residues 1-118). (PDF 87 kb)

Supplementary Fig. 2

HSQC spectra of the 15N labeled MDM2 (residues 1-118). (PDF 104 kb)

Supplementary Fig. 3

Superposition of HSQC spectra of 15N labeled p53 in 5% DMSO (red) and after addition of RITA (blue). (PDF 135 kb)

Supplementary Methods (PDF 146 kb)

Supplementary Note (PDF 336 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Krajewski, M., Ozdowy, P., D'Silva, L. et al. NMR indicates that the small molecule RITA does not block p53-MDM2 binding in vitro. Nat Med 11, 1135–1136 (2005) doi:10.1038/nm1105-1135

Download citation

Further reading