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Intracellular expression of antibody fragments directed against HIV reverse transcriptase prevents HIV infection in vitro

Nature Medicine volume 1, pages 667–673 (1995)Cite this article

Abstract

We have tested a novel strategy of intracellular immunization to block human immunodeficiency virus (HIV) infection. The expression of a specific antibody within a cell was achieved by transduction of genes that encode for immunoglobulin chains with specificity to viral reverse transcriptase. We demonstrated that inhibition of this enzyme makes cells resistant to HIV infection by blocking an early stage of viral replication. If high efficiency transduction with a stable vector into lymphohaematopoietic stem cells or mature lymphocytes can be achieved, gene transfer-mediated intracellular immunization might be a feasible treatment strategy in AIDS.

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Authors and Affiliations

  1. Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 7C103, Bethesda, Maryland, 20892-1652

    Jaroslaw P. Maciejewski, Frank F. Weichold & Neal S. Young

  2. Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892-4255

    Andrea Cara, Davide Zella, Marvin S. Reitz Jr & Robert C. Gallo

Authors
  1. Jaroslaw P. Maciejewski
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  2. Frank F. Weichold
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  3. Neal S. Young
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  4. Andrea Cara
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  5. Davide Zella
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  7. Robert C. Gallo
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Maciejewski, J., Weichold, F., Young, N. et al. Intracellular expression of antibody fragments directed against HIV reverse transcriptase prevents HIV infection in vitro. Nat Med 1, 667–673 (1995). https://doi.org/10.1038/nm0795-667

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