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Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans


A functional comparison was made between a monoclonal secretory antibody generated in transgenic plants and its parent murine IgC antibody .The affinity constants of both antibodies for a Streptococcus mutans adhesion protein were similar. However the secretory antibody had a higher functional affinity due to its dimeric structure. In the human oral cavity, the secretory antibody survived for up to three days, compared with one day for the IgG antibody. The plant secretory antibody afforded specific protection in humans against oral streptococcal colonization for at least four months. We demonstrate that transgenic plants can be used to produce high affinity, monoclonal secretory antibodies that can prevent specific microbial colonization in humans. These findings could be extended to the immunotherapeutic prevention of other mucosal infections in humans and animals.

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  1. 1

    Arntzen, C.J. High-tech herbal medicine: plant-based vaccines [news]. Nature Biotech. 15, 221–222 (1997).

    Article  CAS  Google Scholar 

  2. 2

    Hiatt, A.C., Cafferkey, R. & Bowdish, K. Production of antibodies in transgenic plants. Nature 342, 76–78 (1989).

    Article  CAS  Google Scholar 

  3. 3

    Ma, J.K-C. et al. Generation and assembly of secretory antibodies in plants. Science 268, 716–719 (1995).

    Article  CAS  Google Scholar 

  4. 4

    Lehner, T., Caldwell, J. & Smith, R. Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infect. Immun. 50, 796–799 (1985).

    PubMed  PubMed Central  CAS  Google Scholar 

  5. 5

    Ma, J.K., Smith, R. & Lehner, T. Use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by Streptococcus mutans . Infect. & Immun. 55, 1274–1278 (1987).

    CAS  Google Scholar 

  6. 6

    Ma, J.K-C., Hunjan, M., Smith, R., Kelly, C. & Lehner, T. An investigation into the mechanism of protection by local passive immunisation with monoclonal antibodies against Streptococcus mutans . Infect. Immun. 58, 3407–3414 (1990).

    PubMed  PubMed Central  CAS  Google Scholar 

  7. 7

    Ma, J.K-C., Lehner, T., Stabila, P., Fux, C.I. & Hiatt, A. Assembly of monoclonal antibodies with IgCl and IgA heavy chain domains in transgenic tobacco plants. Eur. J. Immunol. 24, 131–138 (1994).

    Article  CAS  Google Scholar 

  8. 8

    Steward, M.W. & Chargelegue, D. Overview: Measurement and biological significance of antibody affinity and reactivity. In D.M. Weir, L.A. Herzenberg and C.C. Blackwell (eds.), Handbook of experimental immunology, Blackwell Scientific, Oxford, 1997, pp. 38.1.

    Google Scholar 

  9. 9

    Underdown, B.J. & Dorrington, K.J. Studies on the structural and conformational basis for the relative resistance of serum and secretory immunoglobulin A to proteolysis. J. Immunol. 112, 949–959 (1974).

    PubMed  CAS  Google Scholar 

  10. 10

    Mestecky, J. & McGhee, J.R. Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response. Adv. Immunol. 40, 153–245 (1987)

    Article  CAS  Google Scholar 

  11. 11

    Corthesy, B. Recombinant secretory IgA for immune intervention against mucosal pathogens. Biochem. Soc. Trans. 25, 471–475 (1997)

    Article  CAS  Google Scholar 

  12. 12

    Ma, J.K., Hunjan, M., Smith, R. & Lehner, T. Specificity of monoclonal antibodies in local passive immunization against Streptococcus mutans . Clin. Exper. Immunol. 77, 331–337 (1989)

    CAS  Google Scholar 

  13. 13

    Loe, H. & Silness, J. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol. Scand. 21, 532–551 (1963)

    Article  Google Scholar 

  14. 14

    Silness, J. & Loe, H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol. Scand. 22, 121–135 (1964)

    Article  CAS  Google Scholar 

  15. 15

    Hajishengallis, G., Nikolova, E. & Russell, M.W. Inhibition of Streptococcus mutans adherence to saliva-coated hydroxyapatite by human secretory immunoglobulin A (S-lgA) antibodies to cell surface protein antigen I/II: reversal by IgAI protease cleavage. Infect. Immun. 60, 5057–5064 (1992)

    PubMed  PubMed Central  CAS  Google Scholar 

  16. 16

    Mazanec, M.B., Lamm, M.E., Lyn, D., Portner, A. & Nedrud, J.G. Comparison of IgA versus IgG monoclonal antibodies for passive immunization of the murine respiratory tract. Vir. Res. 23, 1–12 (1992)

    Article  CAS  Google Scholar 

  17. 17

    Childers, N.K., Bruce, M.G. & McGhee, J.R. Molecular mechanisms of immunoglobulin A defense. Ann. Rev. Microbiol. 43, 503–536 (1989)

    Article  CAS  Google Scholar 

  18. 18

    Mazanec, M.B., Nedrud, J.G., Kaetzel, C.S. & Lamm, M.E. A three-tiered view of the role of IgA in mucosal defense. Immunol. Today 14, 430–435 (1993)

    Article  CAS  Google Scholar 

  19. 19

    Beighton, D., Russell, R.R. & Whiley, R.A. A simple biochemical scheme for the differentiation of Streptococcus mutans and Streptococcus sobrinus . Caries Res. 25, 174–178 (1991)

    Article  CAS  Google Scholar 

  20. 20

    Zylber, L.J. & Jordan, H.V. Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J. Clin. Microbiol. 15, 253–259 (1982)

    PubMed  PubMed Central  CAS  Google Scholar 

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Ma, JC., Hikmat, B., Wycoff, K. et al. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat Med 4, 601–606 (1998).

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