Technical Report | Published:

Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury

Nature Medicine volume 18, pages 166171 (2012) | Download Citation

Abstract

Studying regeneration in the central nervous system (CNS) is hampered by current histological and imaging techniques because they provide only partial information about axonal and glial reactions. Here we developed a tetrahydrofuran-based clearing procedure that renders fixed and unsectioned adult CNS tissue transparent and fully penetrable for optical imaging. In large spinal cord segments, we imaged fluorescently labeled cells by 'ultramicroscopy' and two-photon microscopy without the need for histological sectioning. We found that more than a year after injury growth-competent axons regenerated abundantly through the injury site. A few growth-incompetent axons could also regenerate when they bypassed the lesion. Moreover, we accurately determined quantitative changes of glial cells after spinal cord injury. Thus, clearing CNS tissue enables an unambiguous evaluation of axon regeneration and glial reactions. Our clearing procedure also renders other organs transparent, which makes this approach useful for a large number of preclinical paradigms.

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Acknowledgements

We thank K. Dornmair, J. Enes, C. Hojer, A. Kania, D. Neukirchen, K. Olsen, M. Stiess and S. Tahirovic for critically reading the manuscript, V. Duc Ha for technical assistance and R. Brand for his help with Amira. We are indebted to A. Borst and M. Sheng for their support and to J. Sanes (Harvard University) for the GFP-M mice. A.E. was supported by the Marie Curie Association (European Union; RTN MRTN–CT–2,003–504,636). C.P.M. was supported by the Hertie Stiftung. N.J. was supported by the Theodor Körner Fonds. F.B. is a recipient of a Career Development Award from the Human Frontier Science Program. This work was supported by the Max Planck Society, the International Foundation for Research in Paraplegia and additional grants from the Deutsche Forschungsgemeinschaft and the Hertie Stiftung.

Author information

Affiliations

  1. Max Planck Institute of Neurobiology, Axonal Growth and Regeneration, Martinsried, Germany.

    • Ali Ertürk
    • , Farida Hellal
    •  & Frank Bradke
  2. Department of Neuroscience, Genentech, South San Francisco, California, USA.

    • Ali Ertürk
  3. Max Planck Institute of Psychiatry, Munich, Germany.

    • Christoph P Mauch
  4. Deutsches Zentrum für Neurodegenerative Erkrankungen, Axonal Growth and Regeneration, Bonn, Germany.

    • Farida Hellal
    •  & Frank Bradke
  5. Max Planck Institute of Neurobiology, Systems and Computational Neurobiology, Martinsried, Germany.

    • Friedrich Förstner
  6. Max Planck Institute of Neurobiology, Cellular and Systems Neurobiology, Martinsried, Germany.

    • Tara Keck
    •  & Mark Hübener
  7. Medical Research Council Centre for Developmental Neurobiology, King's College London, Guy's Hospital Campus, London, UK.

    • Tara Keck
  8. Technical University Vienna, Institute of Solid State Electronics, Department of Bioelectronics, Vienna, Austria.

    • Klaus Becker
    • , Nina Jährling
    •  & Hans Ulrich Dodt
  9. Center for Brain Research, Medical University of Vienna, Section of Bioelectronics, Vienna, Austria.

    • Klaus Becker
    • , Nina Jährling
    •  & Hans Ulrich Dodt
  10. University of Oldenburg, Department of Neurobiology, Oldenburg, Germany.

    • Nina Jährling
  11. Glial Physiology and Imaging, Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany.

    • Heinz Steffens
    •  & Frank Kirchhoff
  12. Development and Maintenance of the Nervous System, Centre for Molecular Neurobiology, Hamburg, Germany.

    • Melanie Richter
    •  & Edgar Kramer
  13. Molecular Physiology, Institute of Physiology, University of Saarland, Homburg/Saar, Germany.

    • Frank Kirchhoff

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Contributions

A.E. initiated the project, designed the experiments, developed and performed the clearing protocol, performed the surgeries, performed the in vivo confocal and two-photon imaging, analyzed the data, made the figures and videos, performed the statistical tests, and wrote the paper. C.P.M. developed and performed the clearing protocol and performed ultramicroscopy imaging. F.H. performed surgeries and analysis for two-dimensional glia quantification and performed the rat tracing. F.F. developed the automated segmentation, tracking software and analyzed the 3D Sholl data. T.K. and M.H. performed initial two-photon experiments. M.R. and E.K. performed the deep-tissue antibody staining. H.S. and F.K. performed two-photon imaging on double- and triple-transgenic mice. K.B. and N.J. performed the histochemical screen and developed the clearing protocol. H.U.D. constructed ultramicroscopy and supervised C.P.M. F.B. initiated the project, designed experiments, coordinated and supervised the project and wrote the paper. All authors edited the paper.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Frank Bradke.

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–14 and Supplementary Methods

Videos

  1. 1.

    Supplementary Video 1

    Uncleared (left) and cleared (right) spinal cords of GFP-M mice were imaged with two-photon microscopy.

  2. 2.

    Supplementary Video 2

    A cleared spinal cord tissue section of a GFP-M mouse imaged with confocal microscopy.

  3. 3.

    Supplementary Video 3

    3D rotation of the sample shown in Figure 2c.

  4. 4.

    Supplementary Video 4

    3D imaging of the unsectioned spinal cord and caudal section of the medulla from a GFP-M mouse in rostro-caudal direction.

  5. 5.

    Supplementary Video 5

    The CST of a rat after tracing with biotin dextran amine conjugated to rhodamine, cleared and imaged with two-photon microscopy.

  6. 6.

    Supplementary Video 6

    Visualization of a single injured spinal cord of a GFP-M mouse in three different orientations: horizontal, sagittal and cross.

  7. 7.

    Supplementary Video 7

    Two-photon stack of the injured spinal cord from a GFP-M mouse in its entire depth in dorsoventral orientation.

  8. 8.

    Supplementary Video 8

    3D reconstruction and animation of the spinal cord from a GFP-M mouse shown in Figure 3.

  9. 9.

    Supplementary Video 9

    3D reconstruction and animation of the spinal cord from a GFP-M mouse shown in Figure 4, 15 months after injury.

  10. 10.

    Supplementary Video 10

    3D reconstruction and animation of the unlesioned spinal cord from a (TgH(CX3CR1-EGFP)) mouse shown in Figure 5a.

  11. 11.

    Supplementary Video 11

    Two-photon scan of unlesioned spinal cord from a astrocyte-GFP mouse (TgN(hGFAP-EGFP)) in dorsoventral orientation.

  12. 12.

    Supplementary Video 12

    The astrocytes in the spinal cord of TgN(hGFAP-EGFP) mouse scanned by two-photon microscopy in high resolution.

  13. 13.

    Supplementary Video 13

    3D imaging of the cleared spinal cord from a double transgenic animal.

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DOI

https://doi.org/10.1038/nm.2600

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