The ability of HIV-1 to use dendritic cells (DCs) for transport and to transfer virus to activated T cells in the lymph node may be crucial in early HIV-1 pathogenesis. We have characterized primary DCs for the receptors involved in viral envelope attachment and observed that C-type lectin receptor (CLR) binding was predominant in skin DCs, whereas binding to emigrating and tonsil DCs was CD4-dependent. No one CLR was solely responsible for envelope binding on all skin DC subsets. DC-SIGN (DC-specific ICAM-3–grabbing nonintegrin) was only expressed by CD14+CDlalo dermal DCs. The mannose receptor was expressed by CD1ahi and CD14+CDlalo dermal DCs, and langerin was expressed by Langerhans cells. The diversity of CLRs able to bind HIV-1 in skin DCs may reflect their ability to bind a range of microbial glycoproteins.
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Supported by the Australian National Centre for HIV Virology Research (A. L. C.); an Australian Postgraduate Award (to S. T.); an Australian National Health and Medical Research Council grant (to P. U. C.); an NIH MSTP grant (to P. U. C.); a Wellcome trust and ANZ trustees grant (to P. U. C.); the Deutsche Forschungsgemeinschaft (S. P.); NIH grants R0140880 and R0135383 (to R. D.); a Burroughs Wellcome Fund translational research award (to R. W. D.); and an Elizabeth Glaser Scientist award from the Pediatric AIDS Foundation (to R. W. D.). Thanks to R. Berkowicz for tonsils, the staff of the VPSU for normal skin, to M. Taylor and D. Hart for cells and reagents and C. Wolczak for processing the manuscript.
The authors declare no competing financial interests.
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Turville, S., Cameron, P., Handley, A. et al. Diversity of receptors binding HIV on dendritic cell subsets. Nat Immunol 3, 975–983 (2002). https://doi.org/10.1038/ni841
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