Abstract
The B-1 subpopulation of B lymphocytes differs phenotypically and functionally from conventional B-2 B cells. B-1 B cells are proposed to derive from a distinct progenitor, but such a population has not been isolated. Here we identify and characterize a B-1 B cell progenitor whose numbers peaked in fetal bone marrow but were less abundant in postnatal bone marrow. These Lin−CD45Rlo–negCD19+ cells responded to thymic stromal lymphopoietin and 'preferentially' reconstituted functional sIgMhiCD11b+CD5lo–neg B-1 B cells, but not sIgM+CD11b− B-2 B cells, in vivo. These data indicate that the CD45Rlo–negCD19+ population includes B-1 B cell–specified progenitors and support models proposing distinct developmental pathways for B-1 B cells.
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Acknowledgements
We thank M. Teitell and O. Witte for critical reading of this manuscript. Supported by the National Institutes of Health (AI21256 and HL54850). Four-color flow cytometry analysis and cell sorting were done in the Flow Cytometry Core Facility at the University of California at Los Angeles that is supported by the National Institutes of Health (CA16042).
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Supplementary information
Supplementary Fig. 1
Lin−CD45lo/−CD19+ cells are not contaminated with mature sIgM+ cells. (PDF 142 kb)
Supplementary Fig. 2
Distribution of Lin−CD45Rlo/−CD19+AA4.1+ and Lin−CD45R+CD19−AA4.1+ cells in the liver of embryos of different ages. (PDF 126 kb)
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Montecino-Rodriguez, E., Leathers, H. & Dorshkind, K. Identification of a B-1 B cell–specified progenitor. Nat Immunol 7, 293–301 (2006). https://doi.org/10.1038/ni1301
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DOI: https://doi.org/10.1038/ni1301
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