Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors

  • An Erratum to this article was published on 01 September 2004

Abstract

Activation of Toll-like receptors (TLRs) results in a proinflammatory response needed to combat infection. Thus, limiting TLR signaling is essential for preventing a protective response from causing injury to the host. Here we describe how a RING finger protein, Triad3A, acts as an E3 ubiquitin-protein ligase and enhances ubiquitination and proteolytic degradation of some TLRs. Triad3A overexpression promoted substantial degradation of TLR4 and TLR9 with a concomitant decrease in signaling, but did not affect TLR2 expression or signaling. Conversely, a reduction in endogenous Triad3A by small interfering RNA increased TLR expression and enhanced TLR activation. Thus, ubiquitination by Triad3A represents one pathway by which the intensity and duration of TLR signaling is controlled.

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Figure 1: Interaction between Triad3 and TLRs.
Figure 2: Structure, distribution, and protein sequence of Triad3A.
Figure 3: Triad3A is an E3 ubiquitin-protein ligase.
Figure 4: Triad3A regulates degradation of TLRs.
Figure 5: Triad3A negatively regulates TLRs activation in multiple cell types.
Figure 6: Depletion of Triad3A enhances TLR expression and cellular responses to TLR ligands.

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Acknowledgements

We thank J.F. Lo and W.Y. Hu for providing reagents and for discussions, and J.C. Mathison for comments on the manuscript. Supported by National Institutes of Health (AI15136, GM28485 and AI54523 to R.J.U.).

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Correspondence to Richard J Ulevitch.

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Chuang, T., Ulevitch, R. Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. Nat Immunol 5, 495–502 (2004). https://doi.org/10.1038/ni1066

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