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Bipotential B-macrophage progenitors are present in adult bone marrow

Nature Immunology volume 2, pages 83–88 (2001)Cite this article

Abstract

According to the current model of adult hematopoiesis, differentiation of pluripotential hematopoietic stem cells into common myeloid- and lymphoid-committed progenitors establishes an early separation between the myeloid and lymphoid lineages. This report describes a rare and previously unidentified CD45R−CD19+ B cell progenitor population in postnatal bone marrow that can also generate macrophages. In addition to the definition of this B-lineage intermediate, the data indicate that a developmental relationship between the B and macrophage lineages is retained during postnatal hematopoiesis.

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Figure 1: CD45R and CD19 expression in myeloid long-term cultures switched to lymphoid conditions and in primary BM cells.
Figure 2: Cell morphology, phenotype and immmunoglobulin gene rearrangement status of CD45R−CD19+ BM cells.
Figure 3: CD45R−CD19+ cells have B cell developmental potential.
Figure 4: CD45R−CD19+ cells do not have T cell developmental potential.
Figure 5: CD45R−CD19+ cells can generate macrophages.
Figure 6: CD45R−CD19+ cells include bipotential B-macrophage progenitors.

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Acknowledgements

We thank I. Williams for the Cell Sorter operation and G. Crooks and D. Rawlings for critical reading of the manuscript. Supported by the National Institutes of Health (HL60658). The Flow Cytometry Core Facility of the Jonsson Cancer Center is supported in part by the National Institutes of Health (CA16042).

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  1. Department of Pathology and Laboratory Medicine and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, 90095-1732, CA, USA

    Encarnacion Montecino-Rodriguez, Hyosuk Leathers & Kenneth Dorshkind

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Correspondence to Kenneth Dorshkind.

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Montecino-Rodriguez, E., Leathers, H. & Dorshkind, K. Bipotential B-macrophage progenitors are present in adult bone marrow. Nat Immunol 2, 83–88 (2001). https://doi.org/10.1038/83210

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