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Frequency of homozygous deletion at p16/CDKN2 in primary human tumours

Nature Genetics volume 11, pages210–212(1995)Cite this article

Abstract

Many tumour types have been reported to have deletion of 9p21 (rets 1-6). A candidate target suppressor gene, p16 (p16INK4a/MTS-1/CDKN2), was recently identified within the commonly deleted region in tumour cell lines7,8. An increasing and sometimes conflicting body of data has accumulated regarding the frequency of homozygous deletion and the importance of p16 in primary tumours. We tested 545 primary tumours by microsatellite analysis with existing and newly cloned markers around the p16 locus. We have now found that small homozygous deletions represent the predominant mechanism of inactivation at 9p21 in bladder tumours and are present in other tumour types, including breast and prostate cancer. Moreover, fine mapping of these deletions implicates a 170 kb minimal region that includes p16 and excludes p15.

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Author information

Affiliations

  1. Department of Otolaryngology Head and Neck Surgery, Head and Neck Cancer Research Division, Johns Hopkins University School of Medicine, 818 Ross Research Building, 720 Rutland Avenue, Baltimore, Maryland, 21205-2196, USA

    Paul Cairns, Thomas J. Polascik, Yolanda Eby, Kaori Tokino, Joseph Califano, Adrian Merlo, Li Mao, Wayne Koch, Donna Schwab & David Sidransky

  2. Department of Urology, Johns Hopkins Hospital, 600 N. Wolfe Street, Baltimore, MD, 21287, USA

    Thomas J. Polascik, G. Steven Bova & William Isaacs

  3. Mayo Clinic, 200 First Street, SW, Rochester, MN, 55905, USA

    John Herath & Robert Jenkins

  4. Department of Pathology, Johns Hopkins Hospital, 600 N. Wolfe Street, Baltimore, MD, 21287, USA

    William Westra, Edward Gabrielson, Kathleen R. Cho & Lora Hedrick

  5. Massachusetts General Hospital & Harvard Medical School, Building 149, 13th Street, Charlestown, MA, 02129, USA

    Joni L. Rutter & Alan Buckler

  6. Department of Environmental Health Sciences, Division of Occupational Health, Johns Hopkins University School of Hygiene and Public Health, Room 7515, 600 N. Wolfe Street, Baltimore, MD, 21205, USA

    Mel Tockman

Authors
  1. Paul Cairns
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  2. Thomas J. Polascik
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  3. Yolanda Eby
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  4. Kaori Tokino
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  5. Joseph Califano
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  6. Adrian Merlo
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  7. Li Mao
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  8. John Herath
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  9. Robert Jenkins
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  10. William Westra
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  11. Joni L. Rutter
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  12. Alan Buckler
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  13. Edward Gabrielson
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  14. Mel Tockman
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  15. Kathleen R. Cho
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  16. Lora Hedrick
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  17. G. Steven Bova
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  18. William Isaacs
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  19. Wayne Koch
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  20. Donna Schwab
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  21. David Sidransky
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Cairns, P., Polascik, T., Eby, Y. et al. Frequency of homozygous deletion at p16/CDKN2 in primary human tumours. Nat Genet 11, 210–212 (1995). https://doi.org/10.1038/ng1095-210

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