Abstract
Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done1. Species for which radiation hybrid–based transcript maps have been established include human2, rat3,4,5, mouse6, dog7, cat8 and zebrafish9,10. We have established a comprehensive first-generation–placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence-tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.
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Acknowledgements
We dedicate this article to our friend and colleague Rosa Beddington (March 23, 1956–May 18, 2001), a scientist of great biological insight. This work was supported by EEC Contract PL 962414. We thank B. Gorick and the Human Genome Mapping Project at Hinxton, UK, for help with the replication of the 7.5-dpc mouse endoderm library; A.M. Mallon and S. Greenaway of the informatics group at the Medical Research Council, Harwell; and V. Taghavi and E. Sartory for technical assistance in marker typing at MRC Harwell.
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Avner, P., Bruls, T., Poras, I. et al. A radiation hybrid transcript map of the mouse genome. Nat Genet 29, 194–200 (2001). https://doi.org/10.1038/ng1001-194
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DOI: https://doi.org/10.1038/ng1001-194
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