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Selection of a human chromosome 21 enriched YAC sub–library using a chromosome–specific composite probe

Nature Genetics volume 1, pages 284–290 (1992)Cite this article

Abstract

The subdivision of total genomic human yeast artificial chromosome (YAC) libraries into specific chromosome clone collections will greatly facilitate the construction of an integrated genetic, physical and transcriptional map of the genome. We report the isolation of 388 YAC clones from a human library with an average insert size of 620 kilobases (kb) by the hybridization of a composite chromosome 21 probe to a high–density array of YAC clones. Roughly half of these clones hybridize to chromosome 21 by fluorescence in situ hybridization. These clones represent a twofold coverage of the chromosome. The technique offers the potential of sub–dividing whole genomic YAC libraries into their chromosomal elements to produce high–resolution tools for genome mapping.

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

    Mark T. Ross, Dean Nižetić, Catherine Knights, Radost Vatcheva, Neil Burden, Christal Douglas, Günther Zehetner, Antonio Baldini & Hans Lehrach

  2. Centre D'Immunologie de Marseille Luminy, Case 906, 13288, Marseille Cedex, 09, France

    Catherine Nguyen

  3. Yale University School of Medicine, Department of Genetics, 333 Cedar Street, I148SHM, New Haven, Connecticut, 06510, USA

    David C. Ward

Authors
  1. Mark T. Ross
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  2. Dean Nižetić
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  3. Catherine Nguyen
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  4. Catherine Knights
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  5. Radost Vatcheva
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  6. Neil Burden
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  8. Günther Zehetner
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  9. David C. Ward
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  10. Antonio Baldini
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Ross, M., Nižetić, D., Nguyen, C. et al. Selection of a human chromosome 21 enriched YAC sub–library using a chromosome–specific composite probe. Nat Genet 1, 284–290 (1992). https://doi.org/10.1038/ng0792-284

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  • DOI: https://doi.org/10.1038/ng0792-284

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