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An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
European Biophysics Journal Open Access 03 June 2009
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Acknowledgements
We thank M. Cornforth for critical reading of the manuscript and for discussions. We also thank K. Mattern, D. van Gent and W. Vermeulen for providing cells; R.Y. Tsien for providing mCherry; C. Dinant for assistance with various laser-assisted local damaging techniques and C.H. van Oven and R.A. Hoebe for technical assistance. M.S.L. was supported by the Netherlands Organisation for Scientific Research (ZonMW 912-03-012). J.S., P.M.K. and J.A.A. were funded in part by a grant from the Dutch Cancer Society. E.S.W., R.L.U. and S.M.B. acknowledge support from the US National Institutes of Health (CA-09236 and CA-43322), US Department of Energy (DE-FG02-01ER63239) and the US National Aeronautics and Space Administration (NNJ04HD83G). The Radiological Research Accelerator Facility is supported through US National Institute of Biomedical Imaging and Bioengineering grant P41-EB002033.
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Supplementary information
Supplementary Fig. 1
Recruitment of repair proteins in response to laser microirradiation. (PDF 501 kb)
Supplementary Fig. 2
TRF2 recruitment to microirradiated regions in the absence of Hoechst. (PDF 161 kb)
Supplementary Fig. 3
Quantification of fluorescence intensity of TRF2 after longitudinal exposure to one or two α-particles. (PDF 75 kb)
Supplementary Fig. 4
Protein response to localized exposure of 400 α-particles. (PDF 229 kb)
Supplementary Table 1
Summary of protein responses after exposure to various DNA damaging sources. (PDF 76 kb)
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Williams, E., Stap, J., Essers, J. et al. DNA double-strand breaks are not sufficient to initiate recruitment of TRF2. Nat Genet 39, 696–698 (2007). https://doi.org/10.1038/ng0607-696
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DOI: https://doi.org/10.1038/ng0607-696
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