Temperature is a critical environmental stimulus that has a strong impact on an organism’s biochemistry. Animals can respond to changes in ambient temperature through behaviour or altered physiology. However, how animals habituate to temperature is poorly understood. The nematode C. elegans stores temperature experiences and can induce temperature habituation-linked cold tolerance. Here we show that light and pheromone-sensing neurons (ASJ) regulate cold habituation through insulin signalling. Calcium imaging reveals that ASJ neurons respond to temperature. Cold habituation is abnormal in a mutant with impaired cGMP signalling in ASJ neurons. Insulin released from ASJ neurons is received by the intestine and neurons regulating gene expression for cold habituation. Thus, temperature sensation in a light and pheromone-sensing neuron produces a robust effect on insulin signalling that controls experience-dependent temperature habituation.
Temperature is one of the most critical environmental stimuli and causes biochemical changes in the body. Animals including humans can, therefore, respond and habituate to changes in ambient temperature. The nematode Caenorhabditis elegans is a useful model for studying the mechanism of temperature habituation, because of its powerful molecular genetics. Previous study by Murray et al.1 demonstrated that C. elegans has a cold tolerance that is regulated by phospholipid saturation. Savory et al.2 previously reported that cold tolerance is regulated by molecules involved in the ageing pathway, such as FOXO-type transcription factors and PI3 kinase. The essential organs and tissues for systematic regulation of cold tolerance in C. elegans, however, remain unknown.
In this study, we investigated the molecular physiological mechanisms underlying temperature experience-dependent cold tolerance in C. elegans. Our Ca2+ imaging and genetic analyses reveal that light and pheromone-sensing (ASJ) neurons receive temperature input and transmit information through a photo signal-transduction pathway. Insulin released from ASJ neurons is received in the intestine and neurons, which controls cold habituation. Our study describes systematic regulation of cold habituation in a living animal.
As previously reported by Murray et al.1 and Savory et al.2, C. elegans has a cultivation temperature-dependent cold tolerance. Wild-type animals cultivated at 20 or 25 °C were killed by cold shock. In contrast, most wild-type animals cultivated at 15 °C survived after cold shock (Fig. 1a). To examine the conditions of temperature experience-dependent cold tolerance in detail, we used varying cold-shock temperatures (0–4 °C) (Fig. 1b), cultivation temperatures (13–27 °C) (Fig. 1c) and cold-shock times (6–240 h) (Supplementary Fig. 1a–c). Cold tolerance decreased when cultivation temperature was higher, when cold-shock temperature was lower and when cold-shock time was longer. We used 2 °C for 48 h as a typical cold-shock treatment for the majority of the following experiments. To determine whether cold tolerance was established at a specific developmental stage, we performed temperature shift experiments using larvae between the L1 and L4 stages (Fig. 1d,e). We found that a shift of cultivation temperature at larval stages did not severely affect the cold tolerance of adult animals (Fig. 1d,e). To understand how long it takes for cold tolerance to be established in adult animals, we shifted the temperature of adult animals (Fig. 1f,g). Unexpectedly, cold tolerance was established only 2–3 h after the cultivation temperature was changed from 25 to 15 °C (Fig. 1f) or from 20 to 15 °C (Fig. 1g). Furthermore, cold tolerance was diminished 2–3 h after the cultivation temperature was changed from 15 to 25 °C (Supplementary Fig. 1d) or 20 to 25 °C (Supplementary Fig. 1e). Detailed-phenotypic analyses indicated that temperature experience for the formation of cold tolerance can be overwritten within 2–3 h.
We next investigated which tissues were involved in temperature experience-dependent cold tolerance, by examining the phenotypes of various tissue-specific mutants. We found that a mutant with defective unc-104/kinesin in almost all neurons showed abnormal enhancement of cold tolerance after cultivation at 20 °C (Fig. 2a)3,4. By contrast, mutants with impairments in the cuticle or body wall muscle did not display cold tolerance abnormalities (Fig. 2a). These data suggest that temperature experience-dependent cold tolerance is regulated, at least in part, by neurons. A known-neural circuit for thermotaxis behaviour of C. elegans involves temperature sensing and processing5,6. We next examined the cold tolerance of mutants defective in the development or function of the temperature-sensing neurons participating in the thermotaxis neural circuit, AFD and AWC, and their downstream interneurons AIY and RIA (Fig. 2b; Supplementary Fig. 2a)5,6,7. Developmental or functional defects of these component neurons of the thermotaxis circuit did not lead to abnormal cold tolerance (Fig. 2b), suggesting that this known temperature-processing neural circuit is not essential for temperature experience-dependent cold tolerance. We found that the thermotaxis mutant, tax-6, which lacks calcineurin function in many neurons, had abnormal cold tolerance (Fig. 2b)8,9. This abnormality was partially rescued by the expression of the tax-6 gene in sensory neurons as well as in almost all neurons (Fig. 2b, tax-6;Ex[unc-14p:: tax-6] (almost all neurons), tax-6;Ex[tax-6p(1.1 kb)::tax-6] (many sensory neurons (amphid and phasmid))). These results imply that sensory neurons are important for cold tolerance. We therefore measured temperature experience-dependent cold tolerance in mutants with defective sensory neurons (Fig. 2c; Supplementary Fig. 2b). Mutant animals with impaired che-13 and osm-6 genes demonstrated severely abnormal cold tolerance after cultivation at 20 °C (Fig. 2c). Both che-13 and osm-6 genes encode components of an intraflagellar transport complex that is essential for cilium function in the sensory ending of sensory neurons (Supplementary Fig. 2b)10,11,12,13, suggesting that sensory input may be essential for cold tolerance.
To identify essential sensory neurons for temperature experience-dependent cold tolerance (Fig. 2d; Supplementary Fig. 2b), we tested the cold tolerance of mutants defective in specific or multiple sensory neurons. A strong abnormality was observed in tax-4 and tax-2 mutants lacking cGMP-gated channels that are expressed in several sensory neurons, such as AFD, AWC, ASJ and ASI (Fig. 2d; Supplementary Fig. 2b)14. We therefore expressed tax-4 complementary DNA (cDNA) in ASJ, AWC and/or ASI sensory neurons of tax-4 mutants using cell-specific promoters (Fig. 2e, tax-4;Ex[trx-1p::tax-4] (ASJ), tax-4;Ex[str-3p::tax-4] (ASI) and tax-4;Ex[ceh-36p::tax-4] (AWC)). We found that the abnormality of tax-4 mutants was rescued by the specific expression of tax-4 cDNA in a single pair of sensory neurons, ASJ neurons, which are known as light and pheromone-sensing neurons (Fig. 2e)15. In addition, laser ablation of ASJ sensory neurons in wild-type animals induced abnormal cold tolerance, which was similar to the tax-4 mutant phenotype (Fig. 2f). These results suggest that cultivation temperature-dependent cold tolerance is controlled by ASJ sensory neurons, and that ASJ neurons negatively regulate cold tolerance.
We hypothesized that ASJ neurons act as thermosensory neurons. To examine this hypothesis, we used the genetically encoded calcium indicator, cameleon16,17, to perform calcium imaging of the ASJ neuron under temperature changes. The cameleon gene, yc3.60, was expressed in ASJ sensory neurons under the trx-1 promoter. We found that the calcium concentration in ASJ neurons changes in response to temperature (Fig. 3a,b; Supplementary Fig. 2c,d). By contrast, this temperature response was decreased in the tax-4 mutant, which lacks a cGMP-gated channel essential for sensory signalling in ASJ (Fig. 3c,d). The defect in tax-4 mutants was rescued by specific expression of tax-4 cDNA in ASJ neurons, even in the absence of expression in the sensory neurons that regulate thermotaxis, such as AFD, AWC and ASI (Fig. 3c,d). Thus, physiological and genetic analyses suggest that ASJ acts as a temperature-sensing neuron. Moreover, the temperature response of ASJ was normal in the mutant with impaired SNB-1/synaptobrevin (Fig. 3f), suggesting that ASJ responses to temperature changes are cell autonomous and do not require neurochemical input from other neurons.
To analyze whether acclimation temperature alters ASJ neuron responsiveness to the temperature stimulus, we monitored calcium concentration changes in ASJ neurons of wild-type animals that were cultivated at 15, 20 or 25 °C, by calcium imaging. Physiological analysis in this study suggested that the temperature response of ASJ neurons is altered by the cultivation temperature (Fig. 3b). We found that calcium concentration changes in ASJ neurons of 20 or 25-°C-cultivated animals were stronger than those of 15-°C-cultivated animals. These results are consistent with the genetic results in this study that ASJ neurons negatively regulate cold tolerance, where reduced-ASJ activity enhances cold tolerance and increased-ASJ activity reduces cold tolerance.
To determine what molecules are involved in temperature signalling in ASJ neurons, we tested mutants defective in phototransduction in ASJ neurons15. We found that mutants of proteins involved in phototransduction in ASJ neurons, including trimeric G protein alpha subunits (gpa-1, gpa-3 or goa-1), guanylyl cyclase (daf-11; odr-1) or phosphodiesterase (pde-1; pde-2), showed abnormalities in cold tolerance (Fig. 4a,b). Although the specific neuron responsible for these abnormalities in cold tolerance was not determined, these results are consistent with a hypothesis that two sensory modalities, light and temperature, are transduced by shared signalling molecules. A lite-1 mutant, lacking a photoreceptor protein expressed in ASJ neurons, showed normal cold tolerance (Fig. 4a,b) and normal calcium concentration changes in ASJ neurons in response to temperature changes (Fig. 3c)15. Therefore, temperature is probably received in ASJ neurons by other receptors.
We next investigated how ASJ sensory neurons control temperature experience-dependent cold tolerance. We demonstrated that mutants defective in synaptic transmission, including mutants of SNB-1/Synaptobrevin, SNT-1/Synaptotagmin, UNC-64/Syntaxin or EGL-21/Neuropeptide processing enzyme, showed abnormal enhancement of cold tolerance phenotypes that were similar to those of ASJ-defective mutants (Fig. 5a). These results imply that neurotransmission is important for cold tolerance.
Previous reports suggest that cold tolerance is mediated by insulin-signalling pathways that are similar to pathways involved in dauer larva formation and ageing, and other stress tolerances, including oxidative stress18,19, ultraviolet light20, heavy metals21 and microbial infections22,23. However, specific insulin molecules and specific tissues involved in cold tolerance have not been determined. C. elegans has 40 genes encoding insulin-like molecules. We focused on two, DAF-28 and INS-6, which are expressed in ASJ sensory neurons24. We found that mutant animals defective in DAF-28 and INS-6 showed partial enhancement of cold tolerance (Fig. 5b,c; Supplementary Fig. 4c). Expressing daf-28cDNA in ASJ neurons of daf-28 mutants rescued abnormal cold tolerance, while expressing daf-28cDNA in AWC neurons reduced the abnormality but tolerance did not return to wild-type levels (Fig. 5d). Previous reports demonstrated that AWC neurons probably act as a temperature-sensing neuron, and that the endogenous daf-28 gene is not expressed in AWC sensory neurons from wild-type animals6,25. Based on these data, DAF-28 could be released from AWC by temperature stimuli in wild-type animals ectopically expressing daf-28 in AWC. Because the endogenous daf-28 gene is not expressed in wild-type AWC sensory neurons, these results suggest that DAF-28/insulin acts in a non-cell autonomous way, and is partially dependent on the neuron type. Similarly, a non-cell autonomous phenomenon is observed in the secretory-signalling pathways of sensory neurons in C. elegans, such as insulin/INS-1-dependent temperature learning behaviour and neuropeptide-dependent neural discrimination26,27. In both cases, ectopic expression of secretory genes in sensory neurons of mutants partially rescued the abnormal phenotype of mutants.
Localization analysis using fluorescent proteins indicated that DAF-28 was co-localized with SNB-1/synaptobrevin at the synaptic region in ASJ neurons (Fig. 5e). By contrast, abnormal localization of DAF-28 in the cell body was observed in ASJ neurons of mutants with impaired UNC-104/kinesin, which is essential for cold tolerance (Supplementary Fig. 4d). These results imply that DAF-28/insulin is released from the synaptic region, and that DAF-28 is transported by kinesin into the synapse.
To determine the molecules downstream of insulin in temperature experience-dependent cold tolerance, we tested various mutants defective in the known insulin-signalling pathway (Figs 5b,c and 6a). Phenotypic analysis showed that mutants defective in the DAF-2/insulin receptor or its downstream molecules showed abnormal enhancement of cold tolerance (Figs 5c and 6a; Supplementary Fig. 4c)28. These results are consistent with a previous report showing that AGE-1/PI3 kinase and DAF-16/FOXO are involved in cold tolerance2. DAF-2 is the only insulin receptor in C. elegans, while there are about 40 ligands for insulin receptors. Genetic epistasis analysis revealed that two insulins, DAF-28 and INS-6, are both positive agonists that work redundantly on the DAF-2/insulin receptor in cold tolerance, because an ins-6; daf-28 double mutant showed a stronger phenotype than each single mutant (Fig. 5c)25,29. Additional genetic epistasis analysis indicated that INS-1/insulin genetically inhibits the DAF-2/insulin receptor through a negative regulation of INS-6/insulin (Fig. 5c, Supplementary Fig. 4c)26,30. Abnormal increments of cold tolerance in daf-2 mutants were suppressed by mutation in the DAF-16/FOXO-type transcriptional factor (Fig. 5c). We found that daf-16 mutant animals showed reduced cold tolerance after cultivation at 18 °C (Supplementary Fig. 4b), although daf-16 mutant animals did not show decreased cold tolerance after cultivation at 20 or 15 °C (Fig. 5c; Supplementary Fig. 4a). In contrast, overexpression of the daf-16 gene induced abnormal increments of cold tolerance (Fig. 5c (wild type; Is[daf-16])). These results suggest that DAF-2/insulin receptor signalling and DAF-16/FOXO act as negative and positive regulators for cold tolerance, respectively. Because 15 °C-cultivated mutant animals defective in insulin signalling appeared to be cold tolerant (Supplementary Fig. 4a), it is probable that additional signalling pathways exist that lead to cold tolerance.
As previously reported, mutations in the insulin pathway, including the daf-2 mutation, extend lifespan, which induce better tolerance under a variety of stress conditions. An ambient temperature of 2 °C is considered an extremely stressful environment for worms. We tested cold tolerance of the mutants, hsf-1/heat-shock factor, jnk-1/MAP kinase, kgb-1 and kgb-2/MAP kinase, and hsp-16.2, hsp-16.41 and hsp-16.48/heat-shock proteins, which are involved in the stress response. Phenotypic analysis of cold tolerance of these mutants showed that these mutants had a phenotype similar to wild-type animals (Supplementary Fig. 5d), suggesting that these molecules are not necessary for cold tolerance.
Since the insulin-signalling pathway is involved in dauer larva formation, we tested other signalling molecules that are important for dauer larva formation, such as molecular components of transforming growth factor-beta (TGF-β) and steroid hormonal signalling (Fig. 6a)31. However, we did not observe strong defects in temperature experience-dependent cold tolerance in these mutants (Fig. 6a). These genetic experiments suggest that the insulin-signalling pathway is essential for cold tolerance, but that TGF-β and steroid hormone signalling are not essential.
In many organisms, including the nematode, the percentage of different fatty acids in the body is important for the formation of cold tolerance1. A previous report by Murray et al.1 demonstrated that the percentage of fatty acids changed depending on the cultivation temperature, which was important for cold tolerance. In addition, Savory et al.2 showed that FOXO-type transcription factor DAF-16, which is involved in insulin signalling, is important for delta-9 desaturase gene expression, which is important for survival at low temperatures. We therefore measured the fatty acid composition of total lipids in daf-2/insulin receptor mutants. We found that the fatty acid composition of total lipids was different between wild type and daf-2 mutants (Supplementary Fig. 6a,b). These results are consistent with previous reports that DAF-16/FOXO regulates genes involved in lipid production.
Insulin is a secretory molecule, and the insulin receptor DAF-2 is expressed in many tissues, such as neurons, muscles and intestine32. To investigate tissues where DAF-2 functions in temperature experience-dependent cold tolerance, we tested the cold tolerance of daf-2 animals expressing daf-2cDNA in neurons, intestine or muscles. The tissue-specific rescue experiments revealed that the DAF-2/insulin receptor functions in both intestine and neurons (Fig. 6b, daf-2(e1370);Ex[unc-14p::daf-2, ges-1p::daf-2] (neuron and intestine)). These results suggest that the insulin-signalling pathway in intestine and neurons is essential for temperature experience-dependent cold tolerance of animals.
A recent study has shown that worm intestine itself is highly cold sensitive, and that this tissue plays a central role in ageing and the stress response, in which the cold receptor transient receptor potential (TRP) channel encoded by trpa-1 is essential33. We therefore tested the function of trpa-1 and its downstream molecule pkc-2 encoding protein kinase C in cold tolerance. Loss-of-function mutants of trpa-1 and pkc-2 showed normal phenotypes when compared with wild type (Fig. 6c). Also, overexpression of the trpa-1 gene in wild-type intestine did not result in an abnormal phenotype (Fig. 6c (wild type; XuEx601)). Besides, overexpression of the trpa-1 gene in the daf-2/insulin receptor mutant did not suppress abnormalities in the daf-2 single mutants (Fig. 6c). These results indicate that TRPA-1 signalling in the intestine is independent of cold tolerance signalling.
To determine a downstream insulin-signalling pathway for temperature-dependent cold tolerance, we used previous DNA microarray analysis results measuring temperature change-dependent gene expression34. We found that mutants of some genes, such as endonuclease (M60.2) and cysteine protease (cpr-1), showed abnormal cultivation-dependent cold tolerance (Fig. 7a; Supplementary Fig. 5a). Expression levels of these genes were significantly changed in daf-2/insulin receptor mutants (Fig. 7b; Supplementary Fig. 5b), indicating that expression of these genes is directly or indirectly regulated downstream of insulin signalling for temperature experience-dependent cold tolerance.
Because cold tolerance can be overwritten within 2–3 h after the cultivation temperature is altered (Fig. 1f,g; Supplementary Fig. 1d,e), we examined calcium measurements in the ASJ neurons during longer times by extending the calcium imaging assays to measure points at 30 min to 5 h (Fig. 3e). We found that the calcium concentration was at its maximum at 30 min after the temperature changed from 17–23 °C, and was strongly decreased at 1 h. The results of calcium imaging of ASJ neurons over longer time periods after warming (Fig. 3e) showed a prolonged response of ASJ neurons to a step in temperature; it is a sustained response to a higher temperature over 30 min, and it is not a short-term transient response to a changing temperature shown in Fig. 3b. This would be expected in a process having long-term effects on cold tolerance. Physiological calcium imaging analysis in this study suggests that ASJ neurons continue to respond to temperature for at least 30 min (Fig. 3e). Since the cold tolerance phenotype can be replaced within 2–3 h after changing the cultivation temperature (Fig. 1f,g; Supplementary Fig. 1d,e), ASJ-dependent insulin secretion could continue for 30 min to 1 h. After that, downstream events of insulin signalling, such as gene expression and changing cell metabolism in the intestine and neurons, may determine cold tolerance within 2–3 h.
Exploring the mechanisms for temperature habituation of animals is an important challenge in life sciences. The results in this study demonstrated that experience-dependent cold tolerance is regulated by a single sensory neuron, ASJ, known as light and pheromone-sensing neuron. ASJ neurons release insulin, which is received by the intestine and other neurons in C. elegans (Fig. 7c).
Our genetic and physiological analysis suggested that ASJ sensory neurons secrete insulin molecules that result in decreasing cold tolerance in wild-type animals. Thus, ASJ-dependent insulin signalling acts as a negative regulator of cold tolerance. In contrast, our genetic model described a positive regulator, the DAF-16/FOXO-type transcription factor. Because daf-16 single mutants only showed a partial decrement of cold tolerance after cultivation at 18 °C (Supplementary Fig. 4b), we hypothesize that there is additional positive signalling in cold tolerance. As previously reported, DAF-16 regulates the gene expression of the delta-9 desaturase gene, which is important for cold tolerance in many animals, including worms, and impairment of the delta-9 desaturase gene decreases cold tolerance in worms2. Based on these data, we propose two plausible models. In the first model, an unidentified temperature-sensing system in the nervous system and/or other tissues controls positive signalling for cold tolerance, although mutant animals with an impaired nervous system have not shown an abnormal decrement of cold tolerance. Alternatively, we can propose another model, where DAF-16 and an unidentified transcription factor could be activated under default conditions without temperature stimuli. This model is consistent with a previous result, where translocation of DAF-16/FOXO between the nucleus and cytoplasm is not observed following cold stimuli2, but is observed at ambient temperatures. In either model, ASJ-dependent temperature signalling acts as a gain-controller of cold tolerance through negative regulation of positive signalling for cold tolerance. It is also likely that the cold tolerance is accomplished by more complicated mechanisms, although we describe here a simpler plausible genetic and physiological model (Fig. 7c).
Molecular physiological systems are mainly conserved from humans to C. elegans, and the systems found in this study can therefore provide a useful model for studying temperature habituation in other animals. Our current work helps explain a complex mechanism for temperature habituation, from stimulus sensing through the neuronal and intestinal reactions that produce temperature tolerance.
All error bars in the figures, including those in the Supplementary Information, indicate standard error of mean. All statistical analyses in the figures, including those in the Supplementary Information, were performed by one-way analysis of variance for multiple comparisons, which were followed by Dunnet post-hoc tests. All single asterisk (*) and double asterisk (**) in the figures indicate P<0.05 and P<0.01, respectively. P indicates probability.
trx-1p::tax-4 cDNA(pTOM4), str-3p::tax-4 cDNA(pTOM3) and ceh-36p::tax-4 cDNA(pTOM10) used in Fig. 2e include tax-4 cDNA that was PCR amplified from pDEST-tax-4cDNA (a gift from Dr Iino)35 and the 3′-untranslated region of unc-54. In addition, pTOM4 includes the ASJ-specific trx-1 promoter (1.0 kb)36, which was from pQZ37 trx-1p::ICE (a gift from Dr Alcedo). pTOM3 includes the ASI-specific str-3 promoter (3.1 kb) from pQZ36 (str-3p::GFP, a gift from Dr Alcedo). pTOM10 includes the AWC-specific ceh-36 promoter (368 bp) from pAK203.
daf-28 cDNA inserted into pUC57 was purchased from GenScript, a DNA synthesizing outsource service company. The trx-1 promoter of trx-1p::daf-28 cDNA (pTOM6), the str-3 promoter of str-3p::daf-28 cDNA(pTOM7) and the ceh-36 promoter of ceh-36p::daf-28 cDNA (pTOM8) used the same fragment as mentioned above.
To construct DAF-28 fused with fluorescent protein (trx-1p::daf-28::dsRedm (pOTA11)), we deleted the stop codon of daf-28 cDNA from pTOM6 by site-directed mutagenesis, and then the DsRed monomer DNA was inserted between the daf-28 cDNA and the 3′-untranslated region. trx-1p::snb-1::VENUS (pOTA5) was replaced by the trx-1 promoter from the promoter region of pTAN93 (glr-3p::snb-1::VENUS)37.
daf-2 cDNA (Pdpy30-daf-2cDNA) was provided by Dr Iino and Dr Tomioka. For the construction of unc-14p::daf-2cDNA or ges-1p::daf-2cDNA, the PCR-amplified fragment of daf-2 cDNA was inserted into each plasmid including a 1.5-kb unc-14 promoter (from plasmid pTTu14p)39 or a 3.3 kb ges-1 promoter (from plasmid pKDK66)17. PCR-amplified fragments were verified by sequencing.
Temperature experience-inducing cold tolerance assay
Uncrowded and well-fed animals were used for the cold tolerance assay. Two adult animals were placed on a 6-cm plate containing 14 ml of nematode growth medium (NGM) with 2% (w/v) agar, on which Escherichia coli OP50 was seeded; the adult animals were transferred to the outside of plate after 8-12 h; the progeny were cultured for about 144–150 h at 15 °C, 85–90 h at 20 °C or 60–65 h at 25 °C. Approximately 70–150 animals were placed on a plate. The plates containing fully matured but not old adult animals were transferred to 2 °C (Figs 2, 3, 4, 5, 6, 7; Supplementary Figs 3–5) or to a designated cold temperature (Fig. 1; Supplementary Fig. 1) in a refrigerated cabinet (MIR-553, Sanyo, Japan). Temperature was monitored by both a digital thermometer and a mercury thermometer. After 48 h (Figs 2, 3, 4, 5, 6, 7; Supplementary Figs 3–5) or a designated time (Fig. 1; Supplementary Fig. 1), the plates were transferred to room temperature (22–24 °C), and the living or dead animals on the plate were counted.
In vivo calcium imaging
In vivo calcium imaging was performed essentially according to previous reports17,38. Well-fed animals expressing yellow cameleon 3.60 driven by the trx-1 promoter, trx-1p::yc3.60 (pTOM13), were used for calcium imaging. Animals were glued onto a 2% (w/v) agar pad on glass, immersed in M9 buffer and covered by a cover glass. For long time calcium imaging (Fig. 3e), animals were immobilized by 0.1-μm diameter polystyrene microspheres (Polysciences 00876–15, 2.5–5% w/v suspension) on 10% (w/v) agar pads on glass, and covered by a cover glass. After long time calcium imaging, animals were checked to still be alive. Sample preparation was completed within 3 min. The sample was then placed onto a Peltier-based thermocontroller (Tokai Hit, Japan) on the stage of an Olympus IX81 at the initial imaging temperature for 2 min, and fluorescence was introduced into a Dual-View optics system (Molecular Devices, USA). Fluorescence images of donor and acceptor fluorescent protein in yellow cameleon were simultaneously captured using an EM-CCD camera EVOLVE512 (Photometrics, USA). Images were taken with a 100-ms exposure time with 1 × 1 binning. The temperature on the agar pad was monitored by a thermometer system, MATS-5500RA-KY (Tokai Hit). For each imaging experiment, fluorescence intensities were measured using the MetaMorph image analysis system (Molecular Device). Relative changes in intracellular calcium concentration were measured as the change in the acceptor/donor fluorescence ratio of yellow cameleon protein (ratio change). All band pass filters for experiments using yellow cameleon were according to previous reports17.
Germline transformations were essentially performed as described previously40. Germline transformations were performed with co-injection mixes consisting of experimental DNA at various concentrations (5–50 ng μl−1) and pKDK66 ges-1p::nls-gfp (50 ng μl−1), pNAS88 ges-1p::nls-tag rfp (50 ng μl−1), or pRF4 rol-6gf as a transgenic marker41.
Fatty acid composition
Lipids were extracted from synchronized cultures of young adults and transmethylated as described previously1. Fatty acid methyl esters were analyzed by gas–liquid chromatography and identified by comparing peak retention times with authentic standards. Fatty acid compositions are presented on a percentage weight basis.
Quantitative PCR analysis
Wild–type animals and daf-2 mutants were individually synchronized at 15 °C. Animals were transferred to 20 or 25 °C, after cultivation at 15 °C for 5 days until they passed the L1–L3 larval stage, because daf-2 mutants show a constitutive dauer larva formation phenotype at 25 °C. Messenger RNA was isolated using the RNeasy kit (Qiagen). The isolated messenger RNA (100 ng) was reverse-transcribed to cDNA using the iScript Advanced cDNA Synthesis kit for RT-PCR (Bio–Rad). Quantitative PCR was performed using primers specific to the genes, Sso Fast EvaGreen Supermix (Bio–Rad) and a DNA Engine Peltier Thermal Cycler–CFX96 (Bio–Rad). Triplicate samples of each dilution were used for quantitative PCR. The average expression of each gene was normalized to that of the house–keeping gene lmn-1 as a reference34. The following sets of primers were used for the detection of cgt-1; 5′-GCGATTTTTATGAGACCCGATG-3′ and 5′-CGAACATACAACGCCGAGAA-3′, cpr-1; 5′-AGCTGGATGCAAACCATACC-3′ and 5′-GGAACAGCGTAGGCAGAGAC-3′, dhs-4; 5′-CTTCGATGTTTGACGCCACT-3′ and 5′-CGACCATTGCCTGAGCTTT-3′, dmd-7; 5′-AACGCGAACAGCTAAATGGA-3′ and 5′-GCGTGCCCTTTCAGTACAAC-3′, gpa-7; 5′-TCGACGCAGCAATGAATACC-3′ and 5′-TGCCCTCCAACATCTATCACTC-3′, gpdh-1; 5′-GGGTGACAACGGATTATGAGG-3′ and 5′-CCGACGGAACTTGGGTAGAA-3′, M60.2; 5′-AAGAAGAGCCCAGACCACGA-3′ and 5′-CATCCGAGGCAATGAAGTTGT-3′.
Laser ablation experiments
Laser ablation experiments were performed essentially according to previous reports5,6. ASJ neurons were killed in anesthetized wild-type animals at the L2–3 stage using a Micropoint system laser microbeam (Photonic Instruments, USA). After laser operation, each animal that had been grown at 20 °C to an adult stage on the Nematode growth media (NGM) plate with sufficient food, E. coli OP50, was tested for cold tolerance at 2 °C for 48 h.
The C. elegans RNA interference (RNAi) library, which had originally been established by Dr Ahringer, was purchased from Source BioScience42 and used for all RNAi analyses in this study. The feeding RNAi protocol was performed as described43. The eri-1; lin-15B strain was44,45 used as a neuron-sensitized strain for RNAi experiments.
Confocal microscopy analysis
Preparation of samples was according to the following procedure: a 2% (w/v) agarose gel on a micro slide glass was covered with 10 μl of 100-mM NaN3 and then a few adult worms were placed on the gel. Finally, the gel was covered by glass. Fluorescent images were analyzed by confocal laser microscopy (FV1000-IX81 with GaAsP photomultiplier tubes, Olympus), using FV10-ASW software (Olympus).
How to cite this article: Ohta, A. et al. Light and pheromone-sensing neurons regulates cold habituation through insulin signalling in Caenorhabditis elegans. Nat. Commun. 5:4412 doi: 10.1038/ncomms5412 (2014).
Murray, P., Hayward, S. A., Govan, G. G., Gracey, A. Y. & Cossins, A. R. An explicit test of the phospholipid saturation hypothesis of acquired cold tolerance in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA 104, 5489–5494 (2007).
Savory, F. R., Sait, S. M. & Hope, I. A. DAF-16 and Delta9 desaturase genes promote cold tolerance in long-lived Caenorhabditis elegans age-1 mutants. Plos One 6, e24550 (2011).
Hall, D. H. & Hedgecock, E. M. Kinesin-related gene unc-104 is required for axonal transport of synaptic vesicles in C. elegans. Cell 65, 837–847 (1991).
Otsuka, A. J. et al. The C. elegans unc-104 gene encodes a putative kinesin heavy chain-like protein. Neuron 6, 113–122 (1991).
Mori, I. & Ohshima, Y. Neural regulation of thermotaxis in Caenorhabditis elegans. Nature 376, 344–348 (1995).
Kuhara, A. et al. Temperature sensing by an olfactory neuron in a circuit controlling behavior of C. elegans. Science 320, 803–807 (2008).
Ohta, A. & Kuhara, A. Molecular mechanism for trimeric G protein-coupled thermosensation and synaptic regulation in the temperature response circuit of Caenorhabditis elegans. Neurosci. Res. 76, 119–124 (2013).
Kuhara, A., Inada, H., Katsura, I. & Mori, I. Negative regulation and gain control of sensory neurons by the C. elegans calcineurin TAX-6. Neuron 33, 751–763 (2002).
Kuhara, A. & Mori, I. Molecular physiology of the neural circuit for calcineurin-dependent associative learning in Caenorhabditis elegans. J. Neurosci. 26, 9355–9364 (2006).
Haycraft, C. J., Schafer, J. C., Zhang, Q., Taulman, P. D. & Yoder, B. K. Identification of CHE-13, a novel intraflagellar transport protein required for cilia formation. Exp. Cell. Res. 284, 251–263 (2003).
Perkins, L. A., Hedgecock, E. M., Thomson, J. N. & Culotti, J. G. Mutant sensory cilia in the nematode Caenorhabditis elegans. Dev. Biol. 117, 456–487 (1986).
Collet, J., Spike, C. A., Lundquist, E. A., Shaw, J. E. & Herman, R. K. Analysis of osm-6, a gene that affects sensory cilium structure and sensory neuron function in Caenorhabditis elegans. Genetics 148, 187–200 (1998).
Deane, J. A., Cole, D. G., Seeley, E. S., Diener, D. R. & Rosenbaum, J. L. Localization of intraflagellar transport protein IFT52 identifies basal body transitional fibers as the docking site for IFT particles. Curr. Biol. 11, 1586–1590 (2001).
Komatsu, H., Mori, I., Rhee, J. S., Akaike, N. & Ohshima, Y. Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans. Neuron 17, 707–718 (1996).
Liu, J. et al. C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog. Nat. Neurosci. 13, 715–722 (2010).
Miyawaki, A. et al. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388, 882–887 (1997).
Kuhara, A., Ohnishi, N., Shimowada, T. & Mori, I. Neural coding in a single sensory neuron controlling opposite seeking behaviours in Caenorhabditis elegans. Nat. Commun. 2, 355 (2011).
Larsen, P. L. Aging and resistance to oxidative damage in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA 90, 8905–8909 (1993).
Vanfleteren, J. R. Oxidative stress and ageing in Caenorhabditis elegans. Biochem. J. 292, Pt 2,605–608 (1993).
Murakami, S. & Johnson, T. E. A genetic pathway conferring life extension and resistance to UV stress in Caenorhabditis elegans. Genetics 143, 1207–1218 (1996).
Barsyte, D., Lovejoy, D. A. & Lithgow, G. J. Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans. FASEB J. 15, 627–634 (2001).
Garsin, D. A. et al. Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogens. Science 300, 1921 (2003).
Lamitina, S. T. & Strange, K. Transcriptional targets of DAF-16 insulin signaling pathway protect C. elegans from extreme hypertonic stress. American journal of physiology. Cell Physiol. 288, C467–C474 (2005).
Cornils, A., Gloeck, M., Chen, Z., Zhang, Y. & Alcedo, J. Specific insulin-like peptides encode sensory information to regulate distinct developmental processes. Development 138, 1183–1193 (2011).
Li, W., Kennedy, S. G. & Ruvkun, G. daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the DAF-2 signaling pathway. Genes Dev. 17, 844–858 (2003).
Kodama, E. et al. Insulin-like signaling and the neural circuit for integrative behavior in C. elegans. Genes Dev. 20, 2955–2960 (2006).
Ishihara, T. et al. HEN-1, a secretory protein with an LDL receptor motif, regulates sensory integration and learning in Caenorhabditis elegans. Cell 109, 639–649 (2002).
Kimura, K. D., Tissenbaum, H. A., Liu, Y. & Ruvkun, G. daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science 277, 942–946 (1997).
Malone, E. A., Inoue, T. & Thomas, J. H. Genetic analysis of the roles of daf-28 and age-1 in regulating Caenorhabditis elegans dauer formation. Genetics 143, 1193–1205 (1996).
Pierce, S. B. et al. Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family. Genes Dev. 15, 672–686 (2001).
Fielenbach, N. & Antebi, A. C. elegans dauer formation and the molecular basis of plasticity. Genes Dev. 22, 2149–2165 (2008).
Wolkow, C. A., Kimura, K. D., Lee, M. S. & Ruvkun, G. Regulation of C. elegans life-span by insulinlike signaling in the nervous system. Science 290, 147–150 (2000).
Xiao, R. et al. A genetic program promotes C. elegans longevity at cold temperatures via a thermosensitive TRP channel. Cell 152, 806–817 (2013).
Sugi, T., Nishida, Y. & Mori, I. Regulation of behavioral plasticity by systemic temperature signaling in Caenorhabditis elegans. Nat. Neurosci. 14, 984–992 (2011).
Hirotsu, T., Saeki, S., Yamamoto, M. & Iino, Y. The Ras-MAPK pathway is important for olfaction in Caenorhabditis elegans. Nature 404, 289–293 (2000).
Miranda-Vizuete, A. et al. Lifespan decrease in a Caenorhabditis elegans mutant lacking TRX-1, a thioredoxin expressed in ASJ sensory neurons. FEBS Lett. 580, 484–490 (2006).
Tanizawa, Y. et al. Inositol monophosphatase regulates localization of synaptic components and behavior in the mature nervous system of C. elegans. Genes Dev. 20, 3296–3310 (2006).
Ohnishi, N., Kuhara, A., Nakamura, F., Okochi, Y. & Mori, I. Bidirectional regulation of thermotaxis by glutamate transmissions in Caenorhabditis elegans. EMBO J. 30, 1376–1388 (2011).
Ogura, K., Shirakawa, M., Barnes, T. M., Hekimi, S. & Ohshima, Y. The UNC-14 protein required for axonal elongation and guidance in Caenorhabditis elegans interacts with the serine/threonine kinase UNC-51. Genes Dev. 11, 1801–1811 (1997).
Mello, C. C., Kramer, J. M., Stinchcomb, D. & Ambros, V. Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10, 3959–3970 (1991).
Mello, C. C., Kramer, J. M., Stinchcomb, D. & Ambros, V. Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10, 3959–3970 (1991).
Fraser, A. G. et al. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330 (2000).
Kamath, R. S., Martinez-Campos, M., Zipperlen, P., Fraser, A. G. & Ahringer, J. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2, RESEARCH0002 (2001).
Timmons, L. & Fire, A. Specific interference by ingested dsRNA. Nature 395, 854 (1998).
Kennedy, S., Wang, D. & Ruvkun, G. A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427, 645–649 (2004).
Henderson, S. T. & Johnson, T. E. daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans. Curr. Biol. 11, 1975–1980 (2001).
We thank I. Mori, X.Z.S. Xu, J. Alcedo, C.I. Bargmann, Y. Iino, S. Tomioka, T. Takasaki, H. Sakamoto, I. Hope and S. Mitani for sharing DNA constructs and strains; K. Takeda for technical advice; the National Bioresource Project (Japan) and the Caenorhabditis Genetic Center for strains; N. Inoue, T. Inoue, Y. Kobayashi, Y. Kinoshita, M. Endo, K. Kanai and N. Kuwahara for supporting experiments; the members of the Kuhara laboratory for comments on this manuscript and stimulating discussion; Anonymous reviewers for invaluable comments on the manuscript. A.K. was supported by the Toray Science Foundation, the Sumitomo Foundation, the Astellas Foundation, the Senri Life Science Foundation, the Simazu Foundation, the Novartis Foundation, the Mitsubishi Foundation, the Naito Foundation, the Casio Foundation, the Research Foundation for Opto-Science and Technology, the Asahi Glass Foundation, the Hirao Taro Foundation of Konan University, JSPS KAKENHI, grant-in-aid for Young Scientists (A) and grant-in-aid for the Challenging Exploratory Research, and Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. A.O. was supported by the Sasagawa Science Foundation, the Naito Foundation, the Narishige Zoological Science Award and grant-in-aid for JSPS Fellows (KAKENHI), Japan.
The authors declare no competing financial interests.
About this article
Cite this article
Ohta, A., Ujisawa, T., Sonoda, S. et al. Light and pheromone-sensing neurons regulates cold habituation through insulin signalling in Caenorhabditis elegans. Nat Commun 5, 4412 (2014). https://doi.org/10.1038/ncomms5412
This article is cited by
Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons
Nature Communications (2022)
Scientific Reports (2022)
BMC Biology (2020)
OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation
Scientific Reports (2020)
Expression of Ice-Binding Proteins in Caenorhabditis elegans Improves the Survival Rate upon Cold Shock and during Freezing
Scientific Reports (2019)