Abstract
Carbonated hydroxyapatite is the mineral found in vertebrate bones and teeth, whereas invertebrates utilize calcium carbonate in their mineralized organs. In particular, stable amorphous calcium carbonate is found in many crustaceans. Here we report on an unusual, crystalline enamel-like apatite layer found in the mandibles of the arthropod Cherax quadricarinatus (freshwater crayfish). Despite their very different thermodynamic stabilities, amorphous calcium carbonate, amorphous calcium phosphate, calcite and fluorapatite coexist in well-defined functional layers in close proximity within the mandible. The softer amorphous minerals are found primarily in the bulk of the mandible whereas apatite, the harder and less soluble mineral, forms a wear-resistant, enamel-like coating of the molar tooth. Our findings suggest a unique case of convergent evolution, where similar functional challenges of mastication led to independent developments of structurally and mechanically similar, apatite-based layers in the teeth of genetically remote phyla: vertebrates and crustaceans.
Similar content being viewed by others
Introduction
Teeth are exposed to considerable abrasion and mechanical stresses. The structure, mineralogy and composition of these mastication tools are intimately linked to feeding habits and are subject to evolutionary selective pressures. As a result, teeth are usually the hardest parts of the body, with superior wear-resistance and biomechanical toughness. Typically, a tooth can be divided into three structurally important components: a hard and wear-resistant working part, a softer supporting 'cushion' that can withstand the impacting, compressive or tensile stresses and a hard–soft interface that is usually mechanically graded to minimize interfacial stresses1,2,3. Diverse examples for the formation of this tri-element tooth structural approach are known. Among the mollusks, chitons (Polyplacophora) use iron oxide (magnetite)4,5 along with calcium phosphate mineral6 in their teeth while limpets (Gastropoda) form iron oxide (goethite) and silica-laden teeth7. The better-known calcium phosphate (hydroxyapatite)-based mammalian teeth, where a hard enamel layer covers a softer bulk of dentin to form highly efficient cutting and grinding tools3 are of course a benchmark reference for teeth structure. Another interesting example are the self-sharpening sea urchin (Echinoidea) teeth with grinding tips that are made of calcite reinforced with high content of magnesium ions, to a level close to dolomite8,9. Common to all these teeth types is the choice, or compositional modification of minerals for increased hardness of the working surface8 and the exquisite control of their location and ultrastructural organization. The latter is often characterized by fibrillar or elongated needle structures oriented perpendicular to the load bearing direction. Various designs are also found in the interfaces between the hard cover and the underlying softer support layer. A well-known example is the mammalian dentin–enamel junction, a distinct layer that prevents crack propagation from enamel into dentin10,11. An interesting alternative to all these highly mineralized teeth are the sclerotized teeth of Polychaete worms, where the use of mineral (copper biominerals) is sparse12,13 and non-mineralized zinc and copper serve to crosslink and harden a proteinaceous matrix rich in histidine13,14.
In the current study, we investigate the mandible of the arthropod Cherax quadricarinatus (freshwater crayfish). In crustaceans, the mandibles are part of the exoskeleton and are periodically removed and regenerated during each molting cycle that may occur every few days in early growth stages15. In contrast to mammalian bone and dentin, which are collagen-based tissues with embedded carbonated hydroxyapatite crystals, the exoskeletons of crustaceans are chitin-based and commonly reinforced with calcium carbonate16. The cuticle of C. quadricarinatus is known to contain amorphous calcium carbonate (ACC)15,17, which can be easily dissolved before the molting as compared with its crystalline polymorphs15,18. We found that the mandibles of the freshwater crayfish contain more than only ACC mineral. They are in part covered by a layer of fluorapatite (FAP) crystals, reminiscent of the hydroxyapatite-based enamel found in vertebrate teeth. We studied the crayfish mandibles on various length-scales and discovered that the complex functional tooth structure is quickly grown by carefully combining amorphous and crystalline minerals. The appearance of an enamel-like apatite layer and similarities in the investigated mechanical properties of the molar crayfish tooth and mammalian teeth suggest a unique case of convergent evolution. Similar functional challenges of needing to grind food, led to remarkably similar solutions that appeared independently in genetically remote phyla: the vertebrates and this ancient group of arthropods19.
Results
Four different minerals found in the mandible
In crayfish, the mandibles form the anterior mouthparts, located directly in front of the oral opening (Fig. 1a). The mandibles consist of a large base, capped with two protuberances: the molar ridge, which serves as a massive grinding surface for mastication, and the incisor process, which serves for grabbing and holding food20 (Fig. 1b). The anterior molar ridge of C. quadricarinatus contains a large tooth that is distinct from the rest of the mandible in both colour and form (Fig. 1c). Raman spectroscopy (Fig. 1d) revealed that the mandible contains four different minerals: ACC and amorphous calcium phosphate (ACP) in the base of the mandible, calcite in the incisor and, most surprisingly, FAP in the anterior molar. Figure 1e shows an X-ray tomography reconstruction of such a mandible. The significantly higher density of the molar (due to higher X-ray attenuation) corresponds to the bright appearance of the tissues observed in Fig. 1c.
The microstructure of the molar tooth
Phase contrast-enhanced synchrotron-based X-ray microtomography revealed the 3D internal microstructure of the molar (Fig. 2a), combining the effects of density variations with contrast arising because of the diffraction of X-rays at interfaces within the specimen21. A virtual slice through the tomogram of a water-immersed typical tooth (Fig. 2a) highlights two different zones, namely the apatite high-density mineral layer (light grey, to the right) and the base of the mandible (medium grey, centre), consisting of lower-density chitin and amorphous mineral. The outermost grinding surface is found on the right hand side of the image (delineated by the white–black line caused by X-ray interference effect). The apatite layer contains an array of parallel channels arranged normal to the outer surface, in a radial direction. These channels are similar and analogous to the crustacean cuticular pore canal system that is formed by cellular extensions of the epithelial cells22. The channels cross the cuticular 'basal layer', running through the amorphous mineral/chitin bulk, gradually thinning out towards the surface and finally terminating below the external tooth surface. Figure 2b is a low-vacuum environmental scanning electron micrograph depicting a diagonal polished section of the transition zone between the apatite (bright on the lower right side) and the amorphous mineral reinforced chitin (dark). This section reveals typical details seen in cross-sectional slices at various depths beneath the outer surface. It is observed that the channels are situated about 6–8 μm apart, with the apatite mineralization beginning sparsely as isolated islands appearing between canals (Fig. 2c). Closer to the outer surface, apatite replaces the amorphous carbonate minerals in close proximity to the channels. The two mineral phases, the external apatite layer and internal chitin-amorphous mineral layer are interdigitated and form a convoluted interface. Conventional secondary electron scanning electron microscopy (SEM) of a fracture-surface (Fig. 2d) revealed elongated apatite crystals (typical size: 0.5 μm thickness ×3 μm length) oriented parallel to the pore canals direction. The 'grainy' matter seen surrounding the canals is presumably the amorphous mineral.
The distribution and orientation of mineral and chitin
A polished cross-section through a crayfish molar tooth (light micrograph Fig. 3a) was imaged by means of microbeam scanning wide-angle X-ray scattering (WAXS) measurements. The micro-diffractograms provided mineralogical identification, relative abundance and orientation information, presented for chitin (Fig. 3b), amorphous minerals (Fig. 3c) and apatite (Fig. 3d). Examples of typical diffraction patterns are shown for the mandible base (Fig. 3e), the transition (Fig. 3f) and apatite zones (Fig. 3g) of the organ. The positions of the measurements are indicated by circles in Fig. 3b–d. These patterns show a diffuse ring due to amorphous mineral, as well as chitin and apatite diffraction arcs that were used to derive the pictures presented in Fig. 3b–d. The apatite crystals are roughly co-aligned with their c-axes orthogonal to the outer surface throughout the entire apatitic layer. Two chitin fibrous components are observed: fibres running parallel to the molar tooth surface in the deeper layer, corresponding to the known cuticle 'twisted plywood' structure23 (also delineated in Fig. 2b by dashed lines), and fibres preferentially oriented orthogonal to the surface24, running parallel to the channel radial direction in the region adjacent to the interface with the apatite (black lines in Fig. 3b showing the predominant chitin c-axis orientation changing close to the grinding surface). The amorphous mineral content increases towards the outer part of the tooth structure (Fig. 3c).
The distribution of carbonate and phosphate
The local chemical composition along the tooth section was studied by Raman spectroscopic imaging and the data are presented in Fig. 4 (a second region is given in the Supplementary Fig. S1). The position of the analysed region (light micrograph shown in Fig. 4a) with respect to the WAXS measurements is indicated by the black rectangle in the light micrograph in Fig. 3a. The images in Fig. 4b–d show the intensities of the phosphate band (Fig. 4b), the carbonate-to-phosphate ratios (Fig. 4c) and the shifts of the centre-of-mass of the phosphate peak (Fig. 4d). Phosphate is found within the entire measured region extending from the apatite layer deep into the amorphous mineral, with a strong gradient at the interface between the bulk of the molar and the outer apatite layer (Fig. 4b). The carbonate/phosphate ratio shows a pronounced gradient even within the amorphous mineral phase, far from the molar surface, with several fluctuations (Fig. 4c) that may reflect temporal physiological variations at the time of deposition. The phosphate ν1 peaks around 950–965 cm−1 consist of two, partially overlapping peaks, that is, a sharper peak at 965 cm−1 of the FAP covering layer, and a broader peak centred at 951 cm−1 of the underlying ACP rich layer. The shift in peak position is the outcome of a gradual increase in FAP near the transition zone, (Fig. 4a (sampling pts 2–5) and Fig. 4e). The abrupt transition seen in Fig. 4d marks the line where FAP abundance overrides ACP. Indeed, the shift to lower wavenumbers is associated with a broadening of the carbonate and phosphate ν1 vibrational modes (as compared with calcite and FAP standards, Fig. 4e), and is characteristic of an amorphous, disordered mineral phase25,26. According to Fig. 4c, the carbonate content in the crystalline apatite is negligible but dominant in the underlying ACC/ACP layer, where both minerals coexist. Moreover, the position of the phosphate band in the Raman spectrum of the apatite region of the tooth corresponds to a band shift usually associated with high fluoridation of apatite27. High-resolution powder X-ray diffraction measurements (Supplementary Fig. S2) and Energy dispersive spectroscopy (EDS) analysis (Supplementary Table S1) further corroborate that the crayfish molar is covered by FAP.
The mechanical properties of the molar tooth
The mechanical properties of a molar tooth cross-section (Fig. 5a) were tested by nanoindentation (Fig. 5b). We found that the hardness systematically changes from 4.5–5 GPa in the outer apatite layer to about 1 GPa in the base of the mandible that consists of chitin and amorphous mineral. A typical scan is plotted in Fig. 5b (open triangles), where a small gradient from about 1–2 GPa is seen in the base of the mandible reflecting the increase in amorphous mineral content, before the values sharply increase at the interface with the FAP layer. Hardness values in the FAP layer are significantly higher than what was previously reported for insect mandibles (3 GPa)28 although the hardness of the base of the mandible is in the range (although slightly larger) of what was found in the crushing part of the lobster claw (0.5 GPa)29. The observed hardness profile is also remarkably similar to that of mammalian teeth30, which consist of dentin, with a hardness of about 0.7 to 1 GPa (refs 31,31) and enamel, with reported hardness values ranging from 3–6 GPa, depending on the direction of the nanoindentation32 and position33 in the tooth. The reduced moduli of the crayfish molar, evaluated from the unloading curves of the nanoindentation, follow the same trend as the hardness (Fig. 5b, full circles). The apatite layer is on average more than three times as stiff and four times as hard as the underlying composite of chitin and amorphous mineral (Table 1).
The nanoindentation measurements of the crayfish molar were performed on a dry sample and are therefore correlated to, but may not precisely reflect the properties of the teeth in their hydrated natural state29. However, the mechanical properties of mineralized cuticle, and especially the densely mineralized crustaceans mouthparts are dominated by their constituent minerals and are therefore less susceptible to the effects of dehydration34,35. Hence our measurements are probably in fair agreement with their natural state.
Discussion
Our results show that the molar of C. quadricarinatus is a highly complex organ that possesses a range of interesting functional features, including the three dental elements of hard cover, soft support, and graded interface between these layers. The observed similarities in hardness values between the crayfish molar and mammalian teeth exist despite the very different mineralogical composition of the supporting material (chitin and amorphous minerals vs collagen and hydroxyapatite), the very remote taxonomic context and the different diets processed by the two types of teeth. Most interestingly, the hard outer layer of the molar crayfish tooth is composed of highly crystalline FAP prisms, oriented normal to the surface, along the co-oriented pore canals and parallel to the load direction. This structural solution for the formation of a hard, wear-resistant surface is remarkably similar to the solution evolved in vertebrates' enamel, which consists of oriented bundles of elongated hydroyapatite crystals. The concept of reinforcing the outermost layer of a tooth with oriented, elongated crystals is relatively widespread. Nevertheless, the different examples described in the introduction show that many invertebrates have evolved completely different solutions based on a variety of minerals and other components. Importantly, from an evolutionary perspective, the appearance of such highly crystalline apatite in crustacean teeth is unexpected. Apatite is commonly well documented in two evolutionary groups: the Chordate (vertebrates) and a small class of invertebrates from the brachiopods (Lingulata)36. The crustaceans, like most of the invertebrates use calcium carbonate for exoskeleton reinforcement. A few exceptions of apatite formation in invertebrates were reported6,37. However, the apatite found in these invertebrates is nanocrystalline and is reminiscent of lamellar bone37. Remarkably, despite the great taxonomic distance, the crayfish molar apatite layer is composed of large apatite crystals, oriented parallel to the load direction, comparable in their mechanical performance to vertebrates' enamel30,32. The use of FAP in the crayfish molar as opposed to hydroxyapatite in mammalian teeth presumably is of advantage for the crustacean. FAP differs from hydroxyapatite only in the substitution of hydroxyl groups by fluorides38, yet has similar mechanical properties27. Fluoridation was previously also reported in the enameloid apatite of sharks39,40. Because of a lower water solubility41,42, the fluoridation of apatite presumably increases the durability of the teeth of C. quadricarinatus, which are mostly in contact with fresh water, rather than saliva as in mammals.
The hard crown is mounted on a softer base of parallel oriented lamellar chitin reinforced with ACC, the typical structure of the crustacean exoskeleton. ACC is widely used in crustacean cuticles, presumably because of its high solubility. This property facilitates the periodic dissolution of the mineral before each molting cycle15. Besides ACC, many crustaceans also use calcite to reinforce their exoskeletons. However, the coexistence of a crystalline apatite forming the grinding surface with the underlying ACP-/ACC- graded interphase layer is reported here for the first time. Between the crystalline fluoroapatite crown and the soft ACC support, the mineral composition continuously transforms from ACP to ACC. The composition gradient is related directly to the variations in mechanical properties forming a unique type of graded interface. The combined structure of apatite and ACC is a physiological cost-effective solution where the metabolically 'expensive' and less resorbable phosphate is restricted to the outer surface whereas the resorbable 'common' carbonate is used for the bulk of the mandible and the cuticle.
In conclusion, the molar of C. quadricarinatus is a highly complex, periodically renewable organ in which a unique architecture of amorphous and crystalline calcium carbonate and phosphate minerals constitutes a tooth with mechanical properties comparable to mammalian teeth. This is achieved by including gradients in the chemical composition and microstructure combining a highly crystalline apatite cap, reminiscent of enamel, covering a chitin scaffold reinforced with amorphous mineral. The similarities in their properties and underlying structural principles make the crayfish molar and mammalian teeth an interesting example of convergent evolution of a functional tooth structure based on hypermineralized enamel, which has been described as a 'masterpiece of bioceramics'43.
Methods
Animals
C. quadricarinatus crayfish were reared in artificial ponds at Ben-Gurion University, Beer-Sheva, Israel. The animals were anaesthetized in ice-cold water before dissection. Mature teeth were extracted from specimens at an inter-molt stage. SEM experiments were carried out with these as well as with exuvia samples. Ben-Gurion University Ethics Committee has exempted experiments with crustaceans from specific permit requirements
Sample embedding and polishing
Mandibles were kept frozen except during preparation to suppress any possible crystallization of amorphous minerals. Mandibles were embedded in Epofix cold-setting resin with a resin/hardener mixing ratio (w/w) of 25/3, and then sectioned with a slow diamond saw (BUEHLER Isomat, Germany) using petroleum distillate (BUEHLER Isocut fluid) as cooling fluid, because ACC is highly water soluble. Mandible sections were further polished using ethyleneglycol lubricant to reduce thickness and provide a flat surface for Raman imaging and Nanoindentation measurements.
Raman spectroscopy and imaging
Raman spectra reported in Fig. 1d were acquired on a Jobin-Yvon LabRam HR 800 micro-Raman system equipped with a liquid nitrogen cooled detector. A He–Ne laser source (633 nm), a ×50 microscope objective, a 100-μm confocal aperture and a 600-mm−1 grating were used, giving a resolution of 2–4 cm−1.
For Raman spectroscopic imaging, a confocal Raman microscope (CRM200, WITec, Ulm, Germany) equipped with a piezo-scanner was used (P-500, Physik Instrumente, Karlsruhe, Germany) with a ×60 (0.8 NA) objective and a diode-pumped 785-nm near-infrared laser (Toptica Photonics AG, Graefelfing, Germany, laser power 10 mW). The surface was scanned using 500 nm steps, and full spectra for each pixel were acquired (0.1 s integration time) with a thermoelectrically cooled CCD detector (DU401A-BV, Andor, UK) placed behind a grating (300 mm−1) spectrograph (Acton, Princeton Instruments, Trenton, NJ, USA) with a spectral resolution of ~6 cm−1. For spectra in Fig. 4e, 1,200 mm−1 grating (UHTS 300, WITec, spectral resolution ~2 cm−1) and 5 s integration times were used. Laser power (10 mW full beam power at the sample) was adjusted to attain a sufficient signal-to-noise ratio and to avoid sample damage. The ScanCtrlSpectroscopyPlus software (version 1.38, WITec) was used for measurements and WITec Project Plus (version 2.02, WITec) for spectra processing. The phosphate and carbonate images were generated by integrating the intensity of the signal for the wavenumber ranges of phosphate (920–990 cm−1) and carbonate (1040–1125 cm−1). The phosphate band position reported in Fig. 4d was obtained using a centre-of-mass filter to calculate the intensity-weighted spectral position of the 920–990 cm−1 wavenumber range.
Micro-tomography
Molar-containing segments of the mandible were imaged using two microtomogaphy instruments: (a) a laboratory instrument (Skyscan 1072, Skyscan, Kontich, Belgium) operated at 100 keV and magnified to obtain a resolution of 6.5 μm (Fig. 1e). Data were reconstructed using the standard Nrecon reconstruction package (Skyscan) and the reconstructed volumes were visualized or virtually sliced to reveal the internal microstructure (Amira 5.2, Visage Imaging GmbH, Germany). (b) The high-resolution microtomography set-up of the BAMline at BESSY-II storage ring (Helmholtz-Zentrum Berlin, Germany), operated as described elsewhere44 (Fig. 2a). For our samples, we used an energy of 30 keV and 1,200 projections were obtained spanning 180° around the rotation axis. An effective pixel size of 2.2 μm was used, and the detector system was positioned 150 mm behind the sample. The tomographic datasets were reconstructed using the European Synchrotron Radiation Facility program PyHST45 and visualized as mentioned in (a) above.
Scanning electron microscopy
Dried molar teeth were fractured perpendicular to the molar surface, sputter-coated with gold and examined using a JEOL JSM-7400f electron microscope. Slices and polished samples were imaged uncoated in an FEI Quanta 600 using the low-vacuum mode of imaging by the large field gaseous secondary electrons detector, with an acceleration voltage of 2–4 kV.
Microbeam WAXS
Scanning WAXS measurements were performed at the μ-Spot beamline46 (BESSY II storage ring, Helmholtz-Zentrum Berlin). Embedded cross-sections of the molar tooth were scanned with a step size of 50 μm using an energy of 15 keV, while collecting two-dimensional diffraction patterns. The local amount of chitin, amorphous mineral and apatite, visualized in the images in Fig. 3b–d, show the integrated intensities of the isolated orthogonal WAXS peaks of the respective phases of chitin and apatite, and of the diffuse scattering halo for the amorphous phase, in a pseudocolour. These maps provide direct visualization of the spatial distribution of these phases47. The orientation representation of the c-axes of apatite and chitin were obtained from the azimuthal maxima of the apatite (002), and chitin (013) reflection (see ref. 23) in the 2D diffraction patterns. These maxima allow us to determine the planes in which the crystallographic c-axes and the related morphological orientations lie. For apatite, the orientation of the c-axis shows the preferred alignment of the crystals. For chitin, it corresponds to the fibre's orientation. As two chitin fibre orientations within the volume were observed, only the one corresponding to the highest peak was used in the present evaluation and was attributed to the predominant local fibre orientation.
Nanoindentation
Nanoindentation was carried out in ambient conditions using an Ubi nanoindentation instrument (Hysitron) with a Berkovich diamond tip. To obtain hardness and reduced modulus, all load–displacement curves were analysed, using the method described by Oliver and Pharr48. 550 indentations were performed using a maximal load of 5,000 μN across the polished surface. A dwell time of 60 s was applied at peak load to take creep behaviour into account. Relatively large lateral steps of 10 μm between each indentation were used. Thus, plastically deformed zones from previous indentations did not affect our measurements. The effect of topography was minimized by finely polishing the sample surface (see sample embedding and polishing).
Additional information
How to cite this article: Bentov, S. et al. Enamel-like apatite crown covering amorphous mineral in a crayfish mandible. Nat. Commun. 3:839 doi: 10.1038/ncomms1839 (2012).
References
Zaslansky, P. & Weiner, S. in Handbook of Biomineralization, Vol. 3 (eds Epple, M. & Bäuerlein, E.) 183–202 (Wiley-VCH, Weinheim, 2008).
Suresh, S. Graded materials for resistance to contact deformation and damage. Science 292, 2447–2451 (2001).
Lucas, P. W. Dental Functional Morphology: How Teeth Work (Cambridge University Press, Cambridge, 2004).
Lowenstam, H. A. Lepidocrocite, an apatite mineral, and magnetite in teeth of chitons (Polyplacophora). Science 156, 1373–1375 (1967).
Weaver, J. C. et al. Analysis of an ultra hard magnetic biomineral in chiton radular teeth. Mater. Today 13, 42–52 (2010).
Lowenstam, H. A. & Weiner, S. Transformation of amorphous calcium-phosphate to crystalline dahllite in the radular teeth of chitons. Science 227, 51–53 (1985).
van der Wal, P. Structural and material design of mature mineralized radula teeth of Patella-vulgata (Gastropoda). J. Ultrastruct. Mol. Struct. Res. 102, 147–161 (1989).
Ma, Y., Cohen, S. R., Addadi, L. & Weiner, S. Sea urchin tooth design: an 'all-calcite' polycrystalline reinforced fiber composite for grinding rocks. Adv. Mater. 20, 1555–1559 (2008).
Ma, Y. et al. The grinding tip of the sea urchin tooth exhibits exquisite control over calcite crystal orientation and Mg distribution. Proc. Natl Acad. Sci. USA 106, 6048–6053 (2009).
Bechtle, S. et al. Crack arrest within teeth at the dentinoenamel junction caused by elastic modulus mismatch. Biomaterials 31, 4238–4247 (2010).
Imbeni, V., Kruzic, J. J., Marshall, G. W., Marshall, S. J. & Ritchie, R. O. The dentin-enamel junction and the fracture of human teeth. Nature Mater. 4, 229–232 (2005).
Lichtenegger, H. C., Schöberl, T., Bartl, M. H., Waite, H. & Stucky, G. D. High abrasion resistance with sparse mineralization: Copper biomineral in worm jaws. Science 298, 389–392 (2002).
Broomell, C. C. et al. Mineral minimization in nature′s alternative teeth. J. R. Soc. Interface 4, 19–31 (2007).
Lichtenegger, H. C. et al. Distribution and role of trace transition metals in Glycera worm jaws studied with synchrotron microbeam techniques. Chem. Mater. 17, 2927–2931 (2005).
Shechter, A. et al. Reciprocal changes in calcification of the gastrolith and cuticle during the molt cycle of the red claw crayfish Cherax quadricarinatus. Biol. Bull. 214, 122–134 (2008).
Lowenstam, H. A. & Weiner, S. On Biomineralization (Oxford University Press, New York, 1989).
Shechter, A. et al. A gastrolith protein serving a dual role in the formation of an amorphous mineral containing extracellular matrix. Proc. Natl Acad. Sci. USA 105, 7129–7134 (2008).
Addadi, L., Raz, S. & Weiner, S. Taking advantage of disorder: amorphous calcium carbonate and its roles in biomineralization. Adv. Mater. 15, 959–970 (2003).
Glenner, H., Thomsen, P. F., Hebsgaard, M. B., Sorensen, M. V. & Willerslev, E. The origin of insects. Science 314, 1883–1884 (2006).
Garm, A. Mechanical functions of setae from the mouth apparatus of seven species of decapod crustaceans. J. Morphol. 260, 85–100 (2004).
Cloetens, P. et al. Observation of microstructure and damage in materials by phase sensitive radiography and tomography. J. Appl. Phys. 81, 5878–5886 (1997).
Travis, D. F. Structural features of mineralization from tissue to macromolecular levels of organization in Decapod crustacea. Ann. NY Acad. Sci. 109, 177–245 (1963).
Al-Sawalmih, A. et al. Microtexture and chitin/calcite orientation relationship in the mineralized exoskeleton of the American lobster. Adv. Funct. Mater. 18, 3307–3314 (2008).
Raabe, D., Al-Sawalmih, A., Yi, S. B. & Fabritius, H. Preferred crystallographic texture of alpha-chitin as a microscopic and macroscopic design principle of the exoskeleton of the lobster Homarus americanus. Acta Biomater. 3, 882–895 (2007).
Kazanci, M., Fratzl, P., Klaushofer, K. & Paschalis, E. P. Complementary information on in vitro conversion of amorphous (precursor) calcium phosphate to hydroxyapatite from Raman microspectroscopy and wide-angle X-ray scattering. Calcified Tissue Int. 79, 354–359 (2006).
Raz, S., Hamilton, P. C., Wilt, F. H., Weiner, S. & Addadi, L. The transient phase of amorphous calcium carbonate in sea urchin larval spicules: The involvement of proteins and magnesium ions in its formation and stabilization. Adv. Funct. Mater. 13, 480–486 (2003).
Freeman, J. J., Wopenka, B., Silva, M. J. & Pasteris, J. D. Raman spectroscopic detection of changes in bioapatite in mouse femora as a function of age and in vitro fluoride treatment. Calcified Tissue Int. 68, 156–162 (2001).
Hillerton, J. E., Reynolds, S. E. & Vincent, J. F. V. On the indentation hardness of insect cuticle. J. Exp. Biol. 96, 45–52 (1982).
Sachs, C., Fabritius, H. & Raabe, D. Hardness and elastic properties of dehydrated cuticle from the lobster Homarus americanus obtained by nanoindentation. J. Mater. Res. 21, 1987–1995 (2006).
Marshall, G. W., Balooch, M., Gallagher, R. R., Gansky, S. A. & Marshall, S. J. Mechanical properties of the dentinoenamel junction: AFM studies of nanohardness, elastic modulus, and fracture. J. Biomed. Mater. Res. 54, 87–95 (2001).
Tesch, W. et al. Graded microstructure and mechanical properties of human crown dentin. Calcified Tissue Int. 69, 147–157 (2001).
Habelitz, S., Marshall, S. J., Marshall, G. W. & Balooch, M. Mechanical properties of human dental enamel on the nanometre scale. Arch. Oral Biol. 46, 173–183 (2001).
Cuy, J. L., Mann, A. B., Livi, K. J., Teaford, M. F. & Weihs, T. P. Nanoindentation mapping of the mechanical properties of human molar tooth enamel. Arch. Oral Biol. 47, 281–291 (2002).
Vincent, J. F. V. Arthropod cuticle: a natural composite shell system. Compos. Part A-Appl. Sci.Manuf. 33, 1311–1315 (2002).
Vincent, J. F. V. & Wegst, U. G. K. Design and mechanical properties of insect cuticle. Arthropod Struct. Dev. 33, 187–199 (2004).
Knoll, A. H. Biomineralization and evolutionary history. Biomineralization 54, 329–356 (2003).
Lowenstam, H. A. & Weiner, S. Phosphatic shell plate of the Barnacle ibla (Cirripedia) - a bone-like structure. Proc. Natl Acad. Sci. USA 89, 10573–10577 (1992).
Wei, M., Evans, J. H., Bostrom, T. & Grondahl, L. Synthesis and characterization of hydroxyapatite, fluoride-substituted hydroxyapatite and fluorapatite. J. Mater. Sci.-Mater. Med. 14, 311–320 (2003).
Daculsi, G. & Kerebel, L. M. Ultrastructural-study and comparative-analysis of fluoride content of enameloid in sea-water and fresh-water sharks. Arch. Oral Biol. 25, 145–151 (1980).
Legeros, R. Z. & Suga, S. Crystallographic nature of fluoride in enameloids of fish. Calcified Tissue Int. 32, 169–174 (1980).
Moreno, E. C., Kresak, M. & Zahradnik, R. T. Fluoridated hydroxyapatite solubility and caries formation. Nature 247, 64–65 (1974).
Okazaki, M., Tohda, H., Yanagisawa, T., Taira, M. & Takahashi, J. Differences in solubility of two types of heterogeneous fluoridated hydroxyapatites. Biomaterials 19, 611–616 (1998).
Hannig, M. & Hannig, C. Nanomaterials in preventive dentistry. Nature Nanotech. 5, 565–569 (2010).
Rack, A. et al. High resolution synchrotron-based radiography and tomography using hard X-rays at the BAMline (BESSY II). Nucl. Instrum. Methods Phys. Res. Sect. A-Accel. Spectrom. Detect. Assoc. Equip. 586, 327–344 (2008).
Mirone, A., Wilcke, R., Hammersley, A. & Ferrero, C. PyHST: high speed tomography reconstruction. Available at http://www.esrf.eu/UsersAndScience/Experiments/TBS/SciSoft.
Paris, O. et al. A new experimental station for simultaneous X-ray microbeam scanning for small- and wide-angle scattering and fluorescence at BESSY II. J. Appl. Crystallogr. 40, S466–S470 (2007).
Al-Sawalmih, A., Li, C. H., Siegel, S., Fratzl, P. & Paris, O. On the stability of amorphous minerals in lobster cuticle. Adv. Mater. 21, 4011–4015 (2009).
Oliver, W. C. & Pharr, G. M. An improved technique for determining hardness and elastic-modulus using load and displacement sensing indentation experiments. J. Mater. Res. 7, 1564–1583 (1992).
Acknowledgements
We thank Oskar Paris, Stefan Siegel and Chenghao Li for support during the scanning WAXS measurements at the μ-Spot beamline and Heinrich Riesemeier and Bernd Müller for kindly providing access to the BAMline beamline, both of the BESSY II storage ring, Helmholtz-Zentrum Berlin. We thank Andy Fitch for help in performing the high-resolution powder diffraction experiments at beamline ID31, European Synchrotron Radiation Facility (ESRF), Grenoble and Igor Zlotnikov for support in the evaluation of the Nanoindentation measurements. We acknowledge financial support from the German Israeli Foundation (GIF), Research Grant No. 950-9.5/2007 and a grant from the National Institute for Biotechnology in the Negev. A.M., P.Z. and P.F. are grateful for support by the Alexander von Humboldt Foundation and the Max Planck Society in the framework of the Max Planck Research Award funded by the Federal Ministry of Education and Research, Germany.
Author information
Authors and Affiliations
Contributions
S.B., P.Z., A.A., A.M., A.B. and B.A. designed and performed the experiments and evaluated the data. These authors as well as P.F. and A.S. contributed significantly to the interpretation of the data, design of figures and writing of the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Information
Supplementary Figures S1 and S2, Supplementary Table S1 and Supplementary References (PDF 199 kb)
Rights and permissions
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
About this article
Cite this article
Bentov, S., Zaslansky, P., Al-Sawalmih, A. et al. Enamel-like apatite crown covering amorphous mineral in a crayfish mandible. Nat Commun 3, 839 (2012). https://doi.org/10.1038/ncomms1839
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/ncomms1839
This article is cited by
-
Mechanical properties, degree of sclerotisation and elemental composition of the gastric mill in the red swamp crayfish Procambarus clarkii (Decapoda, Crustacea)
Scientific Reports (2022)
-
Bioinspired Strategies for Excellent Mechanical Properties of Composites
Journal of Bionic Engineering (2022)
-
Nanoindentation of Supercrystalline Nanocomposites: Linear Relationship Between Elastic Modulus and Hardness
JOM (2022)
-
Genes encoding putative bicarbonate transporters as a missing molecular link between molt and mineralization in crustaceans
Scientific Reports (2021)
-
Living materials fabricated via gradient mineralization of light-inducible biofilms
Nature Chemical Biology (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.