With ageing, there is a loss of adult stem cell function. However, there is no direct evidence that this has a causal role in ageing-related decline. We tested this using muscle-derived stem/progenitor cells (MDSPCs) in a murine progeria model. Here we show that MDSPCs from old and progeroid mice are defective in proliferation and multilineage differentiation. Intraperitoneal administration of MDSPCs, isolated from young wild-type mice, to progeroid mice confer significant lifespan and healthspan extension. The transplanted MDSPCs improve degenerative changes and vascularization in tissues where donor cells are not detected, suggesting that their therapeutic effect may be mediated by secreted factor(s). Indeed, young wild-type-MDSPCs rescue proliferation and differentiation defects of aged MDSPCs when co-cultured. These results establish that adult stem/progenitor cell dysfunction contributes to ageing-related degeneration and suggests a therapeutic potential of post-natal stem cells to extend health.
A universal characteristic of ageing is the loss of regenerative capacity leading to an impaired ability to respond to stress and, therefore, increased morbidity and mortality1. This has led to the hypothesis that ageing is caused, in part, by the loss of functional adult stem cells necessary for tissue regeneration2,3. Indeed, mice greater than two years of age have a significant reduction in the number and proliferative capacity of neural stem cells4,5 and male germline stem cells6,7. In contrast, with ageing, haematopoietic stem cell (HSC) numbers are preserved but their function is lost8,9. Muscle satellite cells also lose stem cell properties with ageing, but their abundance in old age remains controversial10,11,12,13. Ageing-related changes in bone-marrow-derived mesenchymal stem cells (MSCs) include loss of proliferation and differentiation potential, increase in senescence and loss of capacity to form bone in vivo14,15. Similarly, MSCs derived from the bone marrow of patients with Hutchinson–Gilford progeria syndrome, a disease of dramatically accelerated ageing, are defective in their ability to differentiate16. Adipose-derived multipotent cells show an age-dependent loss of self-renewal capacity and an increased propensity for adipogenesis17. Despite this extensive evidence for adult stem cell decline with ageing, it is not known whether old stem cells contribute to ageing-related degenerative changes or are merely a biomarker of it.
Given that ageing-related degenerative changes are universal in the musculoskeletal system18,19,20, we investigated the impact of ageing on murine muscle-derived stem/progenitor cells (MDSPCs). MDSPCs are multipotent cells isolated from postnatal skeletal muscle through an established preplate technique21,22. They have a capacity for long-term proliferation, are resistant to oxidative and inflammatory stress, show multilineage differentiation and self-renew, induce neovascularization, and stimulate regeneration of bone, skeletal and cardiac muscles21,23,24,25. These cells likely reside in the perivascular niche26. These characteristics raise the possibility that the loss of MDSPCs or related perivascular progenitor cells could contribute to sarcopenia, osteoporosis27,28 and other degenerative diseases associated with ageing.
Here we examine MDSPC function in natural ageing and a mouse model of a human progeria. XFE progeroid syndrome is caused by mutations in XPF (xeroderma pigmentosum complementation group F) and characterized by dramatically accelerated ageing of the epidermal, haematopoietic, endocrine, hepatobiliary, renal, nervous, musculoskeletal and cardiovascular systems29. XPF encodes one subunit of an endonuclease that together with its essential binding partner ERCC1 (excision repair cross complementation group 1) participates in numerous DNA repair mechanisms30. XFE progeroid syndrome was modelled in the mouse by mutation of the mErcc1 locus to titrate expression of ERCC1–XPF31,32. Ercc1−/− mice with undetectable levels of ERCC1–XPF have a severely reduced lifespan of 1 month (mth)29,30,32 while Ercc1−/Δ mice, expressing 10% of the normal level of ERCC1–XPF have a lifespan of 7 mths (refs 30, 32). Both strains faithfully mimic the systemic accelerated ageing of XFE progeroid syndrome and demonstrate a highly significant correlation with naturally aged mice at the level of histopathology, pathophysiology and genome-wide transcriptional reprogramming30,32,33,34. Our results indicate that MDSPC function is adversely affected with accelerated and natural ageing. This dysfunction directly contributes to ageing-related degenerative changes since transplantation of functional MDSPCs was sufficient to extend healthspan and lifespan of progeroid mice. Furthermore, we provide evidence that the therapeutic effect of MDSPCs may be mediated by secreted factors that act systemically.
Loss of stemness properties with ageing
MDSPCs are slowly adhering cells isolated from skeletal muscle and enriched through a preplate technique21,22. Previously, we reported isolating these multipotent cells from young (3-week (wk)-old) mice22,35. To determine whether MDSPCs could also be isolated from adult and old mice, we followed the same preplate technique, and the resulting cells were analysed for expression of stem/progenitor cell markers (CD34, Sca-1). Cells isolated from skeletal muscle of young (3-wks), adult (19-wks), and old wild-type (WT) (2-years (yrs)) mice through this technique expressed CD34 and Sca-1 (Fig. 1a), establishing that MDSPCs could be isolated from mice of all ages. Although expression of CD34 appeared mildly attenuated in the older cell populations, Sca-1, which is a more stable marker of MDSPCs36, was similarly expressed in all three populations. Furthermore, MDSPCs isolated from adult WT mice showed multilineage differentiation (Fig. 1b).
To determine whether there is a loss of stem cell function with ageing and progeria, MDSPCs were isolated from old WT mice (2-yrs) as well as progeroid Ercc1−/− (2–3-wks) and Ercc1−/Δ (5-mths) mice and their proliferation and differentiation kinetics measured in vitro. As controls, MDSPCs were isolated from age-matched WT littermates of the progeroid mice and Xpa−/− (xeroderma pigmentosum complementation group A) mice (3-wks), which are defective in nucleotide excision repair of DNA like ERCC1-deficient mice, but do not age rapidly37. To measure proliferation of MDSPCs, an equivalent number of cells from each population were plated, and live-cell imaging (LCI)38 was used to measure growth kinetics (Fig. 1c). Analysis of images captured from the different cell populations revealed that proliferation of MDSPCs isolated from the old WT, Ercc1−/− and Ercc1−/Δ mice, but not Xpa−/− mice, was significantly reduced relative to MDSPCs isolated from young WT mice (*P<0.05, Tukey's test). The average population-doubling time (PDT) was prolonged in MDSPCs isolated from old mice [18 hours (h)] and progeroid mice (20–27 h) compared with young WT mice (15 h) (Fig. 1c). In support of this, the division time (DT) of single cells tracked within the population, or analysed as isolated clones, was also prolonged for old WT-MDSPCs and ERCC1-deficient MDSPCs compared with young WT-MDSPCs (Supplementary Table S1). These data demonstrate that the proliferation capacity of MDSPCs diminishes with age.
To determine whether the capacity for myogenic differentiation is affected in old and progeroid MDSPCs, the cells were cultured to confluence and switched to differentiation medium (low serum). The young WT- and Xpa−/− MDSPCs fused to form multinucleated myotubes expressing fast myosin heavy chain (f-MyHC), a marker of terminal myogenic differentiation (Fig. 1d). In contrast, MDSPCs isolated from old WT and progeroid ERCC1-deficient mice formed significantly fewer and smaller myotubes indicating impaired differentiation (Fig. 1d; *P<0.001; Kruskal–Wallis ANOVA on ranks). The differentiation defect was confirmed by measuring the expression of myogenic differentiation markers MyHC, desmin, and myogenin (Fig. 1e). Myogenic differentiation of individual MDSPC clones from old and progeroid mice was also significantly reduced, compared with young WT mice (Supplementary Fig. S1; *P<0.001, Kruskal–Wallis ANOVA on ranks). Similarly, osteogenic and chondrogenic differentiation of progeroid and old MDSPCs was attenuated compared with cells isolated from young WT mice (Fig. 1b). In contrast, the capacity for adipogenic differentiation was retained in aged MDSPCs. To determine whether the myogenic differentiation defect translated in vivo, MDSPCs isolated from progeroid mice were injected intramuscularly (IM) into mdx mice, a model of Duchenne's muscular dystrophy39. Indeed, cells derived from Ercc1−/Δ mice had an impaired ability to regenerate muscle fibres in dystrophic muscle compared with young WT-MDSPCs (Fig. 1f,g; P<0.001, Mann–Whitney rank sum test). Cumulatively, these data provide evidence of a significant loss of stemness properties, including proliferation under clonogenic conditions, multipotential differentiation and tissue regeneration, in MDSPC populations isolated from old and progeroid mice.
To rule out the possibility that the stem cell dysfunction was an artefact caused by extensive passaging of MDSPCs ex vivo, we measured the number of cells with stem/progenitor surface markers and capacity for myogenic differentiation at the earliest possible time point following isolation from skeletal muscle. Preplate 1 and 2 (pp1–2; Fig. 2a), which constitute all cells directly isolated from skeletal muscle, were induced to differentiate within 24 h of isolation. f-MyHC positive myotube formation was impaired in cells isolated from the skeletal muscle of progeroid ERCC1-deficient mice compared with WT littermates (Fig. 2b). Similarly, expression of a second terminal myogenic differentiation marker, myogenin, was reduced in the ERCC1-deficient cells compared with WT controls (Fig. 2c). Twenty-four-hours post-isolation from skeletal muscle, those cells that remained nonadherent were stained for surface markers Sca-1, CD34 and CD45 and analysed by fluorescence-activated cell sorting (FACS) to quantify the mixed population of non-haematopoietic stem/progenitor cells in the muscle (Fig. 2d). Sca-1 is expressed on adult stem cells, a sub-population of myoblasts40, muscle-derived fibrogenic/adipogenic progenitors41, and a non-myogenic population of cells that emerge in muscle with ageing42.
In young WT mice, ∼20% of the cells were Sca-1+/CD34+/CD45−. In contrast, <10% of the cells isolated from old or progeroid skeletal muscle were Sca-1+/CD34+/CD45−. Averaging data from 3–5 populations of each genotype revealed a significant depletion of this population of cells as animal age (Fig. 2e; *P<0.05; Tukey's test). Next, we plated an equivalent number of these sorted cells to directly compare the myogenic differentiation capacity between Sca-1+/CD34+/CD45− cells from the different genotypes and ages of mice. Myogenic differentiation was significantly impaired in cells isolated from old or progeroid mice compared with young WT mice (Fig. 2f,g; *P<0.001; Kruskal–Wallis ANOVA on ranks). These data demonstrate that there is a loss of MDSPC cell number as well as capacity for myogenic differentiation with ageing.
To determine whether there is a difference in the function of muscle stem cells between aged and young organisms in vivo, we examined muscle regeneration utilizing a well-established cardiotoxin injury-induced model (Supplementary Methods)43. The total area of muscle regeneration, as defined by centronucleated fibres, did not significantly differ between young WT, old WT, and progeroid mice (Supplementary Table S2). However, there was a dramatic difference in the average cross-sectional area of the regenerated myofibres in old and progeroid mice compared with young adult mice (Fig. 3a). More than 50% of the centronucleated, dystrophin-positive myofibres in old and progeroid mice had an area of ≤ 500 μm2 (Fig. 3b). In contrast, >60% of the myofibres in young WT mice had an area of >1,000 μm2, indicative of significantly more mature regenerated myofibres (P<0.001; Kruskal–Wallis ANOVA on ranks). In addition, Masson's Trichrome stain revealed increased fibrosis in muscle of old and progeroid mice in comparison to young WT mice following injury (Fig. 3c,d; *P<0.05; Tukey's test). These data demonstrate that muscle regeneration is delayed and impaired in aged organisms. Collectively, the data in Figures 2 and 3 support the conclusion that aged organisms have a muscle stem/progenitor cell defect.
Treating with young WT-MDSPCs extends life and healthspan
To determine whether the loss of functional MDSPCs contributes to ageing-related degenerative changes, we asked whether transplantation of young WT-MDSPCs affects either the healthspan or lifespan of progeroid mice. Littermate pairs of Ercc1−/− mice were injected intraperitoneally (IP) with 2–4×105 young WT-, progeroid-, or old WT-MDSPCs per gram body weight or PBS at 17 days of age, and lifespan was measured (summarized in Supplementary Table S3). Ercc1−/− mice injected with young WT-MDSPCs showed pronounced weight gain and a significantly extended lifespan compared with sibling mutant animals injected with vehicle only (Fig. 4a; *P<0.05, Dunn's test). In an identical experiment, early passage primary mouse embryonic fibroblasts (MEFs) did not affect the growth or lifespan of Ercc1−/− mice (Fig. 4a). Similarly, MDSPCs isolated from old WT or progeroid Ercc1−/− mice did not have a significant impact on lifespan.
The young WT-MDSPCs were also injected IP into Ercc1−/Δ mice at 6–7 wks of age and again 6 wks later. The age at onset of characteristic progeroid symptoms was measured biweekly, including dystonia, trembling, kyphosis, ataxia, muscle wasting, loss of vision, urinary incontinence and decreased spontaneous activity. The results were compared with littermate Ercc1−/Δ mice treated with vehicle only in a pairwise fashion. The investigators were blinded to the treatment arm. The ageing score, representing the fraction of symptoms that were delayed in mutant mice treated with MDSPCs compared with a mutant littermate treated with PBS, revealed a highly significant difference between treatment groups (Fig. 4b; ***P<0.0008; Student's t-test). These data provide evidence that loss of stem cell function has a direct causal role in ageing-related degeneration.
Young WT-MDSPCs engraft after transplantation
To address the question of how MDSPCs could extend healthspan and lifespan, we asked whether the donor cells engrafted in host tissues. Young WT-MDSPCs, transduced with a retroviral vector containing LacZ with a nuclear localization sequence (nLacZ), were injected IP into Ercc1−/− mice (n=8). Fourteen tissues were analysed (Supplementary Table S4) for the presence of donor cells. LacZ+ cells were detected in the parenchyma of the liver and kidney, as well as connective tissues associated with pancreas and spleen of all mice analysed (Fig. 5). Similar results were obtained in animals euthanized from 1 to 9 wks post-injection. LacZ+ cells were also detected in lung, oesophagus, thymus, bladder/ureter, spinal cord, and bone marrow of at least one mouse (Fig. 5; Supplementary Fig. S2). Importantly, donor cells were not detected in the heart, brain, skeletal muscles, and lymph nodes of any mice. The extent of donor cell engraftment and expansion is limited, indicating that the beneficial effects of MDSPCs is not due to tissue reconstitution by donor cells as is seen with haematopoietic stem cells in bone marrow transplant.
Young WT-MDSPCs restore the function of aged MDSPCs
In a progeria model (Ercc1−/− mice), young WT-MDSPCs had a therapeutic effect. However, the duration of the experiment (<3 wks) and the limited extent of engraftment of IP-injected MDSPCs, as far as we could detect, made it unlikely that this positive effect was a result of substantial MDSPC engraftment, proliferation, differentiation and tissue regeneration. Thus, we next asked whether young WT-MDSPCs confer their therapeutic effects through a paracrine mechanism, requiring secreted factors. MDSPCs isolated from 14-day-old progeroid Ercc1−/− mice were co-cultured with the young WT-MDSPCs, using a transwell system (Fig. 6a). The proliferation of Ercc1−/− MDSPCs was measured for 72 h while the cells were exposed to proliferation media from young WT-MDSPCs. Subsequently, the Ercc1−/− MDSPCs were switched to low-serum media to measure myogenic differentiation. The proliferation defect of Ercc1−/− MDSPCs was significantly improved by co-culture of the cells with young WT-MDSPCs (Fig. 6b; *P<0.001, Mann–Whitney rank sum test). In addition, myogenic differentiation of Ercc1−/− MDSPCs was improved after co-culture with young WT-MDSPCs (Fig. 6c). Likewise, impaired differentiation of old WT-MDSPCs was rescued by conditioned media from young WT-MDSPCs. To quantify the improvement, MDSPCs from progeroid Ercc1−/− or old WT mice were grown in the presence of conditioned media from young WT-MDSPCs, then collected for immunodetection of the terminal myogenic differentiation marker f-MyHC (Fig. 6d). f-MyHC expression was dramatically increased (2.5–9×) if the cells were grown in the presence of media from young WT-MDSPCs compared with differentiation media alone. Thus young WT-MDSPCs were able to rescue, at least in part, the function (proliferation and differentiation) of MDSPCs isolated from progeroid and old mice via secreted factors.
MDSPCs promote neovascularization and tissue regeneration
To determine whether secreted factors were indeed important for the therapeutic effect of MDSPCs, we asked whether there was evidence of improved histopathology in tissues of treated ERCC1-deficient mice in which transplanted MDSPCs were not detected, in particular, skeletal muscle and brain. We previously demonstrated that the regenerative potential of MDSPCs transplanted into skeletal muscle, heart and bone correlates with their ability to induce angiogenesis21,24,25,35,44. Thus, we asked whether IP injection of young WT-MDSPCs had an effect on the vasculature of the brain, as well as muscle fibre size. Muscle sections were stained for dystrophin to highlight muscle fibre perimeter and CD31 to identify endothelial cells within microvessels (Fig. 7a). For each image, the total number of muscle fibres and CD31+ cells were quantified, and the ratio (# of CD31+ cells/# of muscle fibres) determined. Muscles of 15-day-old Ercc1−/− mice had significantly reduced numbers of CD31+ cells at the periphery of muscle fibres compared with WT littermates (Fig. 7b; 0.56 CD31+ cells per fibre versus 1.25 for WT mice; *P<0.001, Student's t-test). The average muscle-fibre size was also significantly reduced in the Ercc1−/− mice (324 μm2) compared with WT littermates (Fig. 7b; 734 μm2, *P=0.002, Mann–Whitney rank sum test).
IP injection of young WT-MDSPCs into Ercc1−/− mice led to a significant increase in the number of CD31+ cells 9 wks post-injection compared with untreated Ercc1−/− mice that only live 3–4 wks (Fig. 7b; 1.05 CD31+ vessels/fibre; §P<0.05, Tukey's test). Similarly, muscle-fibre size significantly increased (Fig. 7b; 1,580 μm2 cross-sectional area; §P<0.05, Dunn's test). It is impossible to directly compare the fibre size between Ercc1−/− mice transplanted with young WT-MDSPCs to untreated mutant animals of the same age because the latter don't live to 9 wks of age (Fig. 4a). Nevertheless, muscle regeneration and growth was evident in Ercc1−/− mice treated with MDSPCs.
Progeroid Ercc1−/− mice show reduced vascularization in the brain compared with WT littermates at 3 wks of age (Fig. 7c; Supplementary Fig. S3). Although donor cells were not detected in the brain of Ercc1−/− mice, cell transplantation significantly improved neovascularization in the brain of these progeroid mice. In 3-wk-old Ercc1−/− mice, the median area of cerebral cortex occupied by vasculature was 1.2%. In Ercc1−/− mice injected with MDSPCs, vascularization increased approximately to the levels in 3-wk-old WT mice (2.2 versus 2.5%, respectively) and the improvement was sustained. Ercc1−/− mice treated with MDSPCs that survived to 9 wks of age, had a vascular area indistinguishable from age-matched WT mice (3.2 versus 3.1%, P<0.05, Dunn's test). These data demonstrate that there is improvement in the histopathology of several tissues in which donor cell engraftment was not detected, suggesting that MDSPCs exert their therapeutic effect through secreted factors.
Using IM injection of nLacZ-labelled MDSPCs to localize donor-cell engraftment, we analysed host muscle for presence of LacZ+ myofibres and endothelial cells (Fig. 8). Like IP, IM administration of young WT-MDSPCs into Ercc1−/− mice (Fig. 8a, n=3 mice per group) led to a significant increase in the number of CD31+ cells (Fig. 8b; 1.06 CD31+ vessels per fibre; §P<0.05, Tukey's test) compared with the non-injected Ercc1−/− mice (0.56 CD31+ vessels/fibre). The muscle-fibre cross-sectional area was also significantly increased (Fig. 8b; 522 versus 324 μm2, §P<0.05, Dunn's test). At the site of injection, numerous myofibres with central or peripheral LacZ+ nuclei were identified, demonstrating that transplanted MDSPCs have the capacity to integrate into regenerated muscle fibres (Fig. 8c). In addition, analysis of sections of the gastrocnemius distant from the injection site revealed a shift in the distribution of myofibre size towards larger regenerating fibres compared with contralateral non-injected muscle (Fig. 8d; P<0.001, Mann–Whitney rank sum test at 151–300 and 301–450). This suggests that IM injection of MDSPCs also affected host myofibres through a paracrine mechanism, requiring secreted factors. Sections were also stained with X-gal and for CD31 to determine whether donor cells co-localize with CD31+ cells (Fig. 8e). Background staining in Ercc1−/− mice was extensive, compared with mdx/SCID mice, likely due to interstitial fibrosis. In both cases, there was a lack of co-localization of LacZ and CD31 staining. These data are consistent with our previous findings25,45 and the conclusion that transplanted MDSPCs do not directly contribute to blood vessels and thus likely drive host angiogenesis through secreted factors.
MDSPCs are stem/progenitor cells isolated from skeletal muscle, a tissue that universally declines in mass and function with ageing20. This cell population cannot be discriminated from less multipotent progenitors strictly based on surface markers, and thus are difficult to quantify. Nevertheless, MDSPCs were isolated from old WT mice and mice with accelerated ageing, using a well-established preplate technique21,22. In both cases, cell proliferation and multilineage differentiation is significantly impaired relative to MDSPCs isolated from young WT mice (Fig. 1). Furthermore, fewer cells expressing stem/progenitor cell markers are isolated from skeletal muscles of aged and progeroid mice (Fig. 2). Finally, muscle from aged and progeroid mice has significantly reduced regenerative capacity following injury (Fig. 3; Supplementary Table S2), consistent with previous reports11. These data establish that the function of MDSPCs is compromised with ageing, similar to neuronal, mesenchymal, haematopoietic, satellite, and adipose stem cells4,15,17,46,47,48. The similarity of deficits in MDSPCs from old WT and progeroid mice extends the commonalities between accelerated ageing caused by a defect in DNA repair and natural ageing. Similar parallels were found between Hutchinson-Gilford progeria syndrome and ageing in the MSC compartment49.
Numerous studies provide evidence that the number and/or function of diverse adult stem cell populations decline with ageing4,6,7,15,47,50,51. However, these are largely correlative studies that do not discriminate whether changes in the stem cell compartment have a causative role in ageing or are merely a consequence of ageing. Genetic studies revealed proteins and pathways essential for the maintenance of stem cell function. Mutation of these genes leads to foreshortened lifespan and early onset of some ageing-related pathologies. For example, mice deficient in the polycomb protein BMI-1 have a short lifespan52, along with defects in self-renewal of HSCs, leading to adult stem cell depletion53. Similarly, mice deficient in HMGA2 show reduced stem cell numbers and function (self-renewal) throughout the central and peripheral nervous systems54. Finally, HSC function and regenerative capacity are significantly diminished in mice harbouring mutations in diverse DNA repair genes or telomerase (Lig4Y288C, Ku80−/−, XpdTTD, and mTR−/−) indicating that genome and telomere maintenance are crucial for stem cell function55,56. However, there is no evidence that loss of stem cell function is directly responsible for decreased lifespan and ageing-related pathology in these models. Here we demonstrate that transplantation of functional WT stem/progenitor cells isolated from juvenile mice into hosts harbouring dysfunctional MDSPCs leads to a significant extension of healthspan and lifespan (Fig. 4). These results provide experimental evidence that the loss of adult stem cell function directly contributes to ageing-related phenotypes.
Interestingly, the function of MDSPCs from aged mice could be restored if they were co-cultured with young WT-MDSPCs (Fig. 6). Similar results were seen in vivo using parabiosis47. These observations demonstrate that old, dysfunctional MDSPCs can be rescued by exogenous factors, rather than the cells being inherently defective. This is in contrast to aged haematopoietic stem cells, which appear to have an intrinsic defect (reviewed in ref. 57).
IP administration of young WT-MDSPCs to progeroid mice promoted growth of the animals, decreased skeletal muscle atrophy, and stimulated neovascularization in the brain and muscle, despite the fact that donor cells were not detected in these tissues (Figs 4 and 7). In addition, transplantation of young WT-MDSPCs directly into progeroid muscle led to muscle regeneration as well as host neovascularization, demonstrating a paracrine mechanism requiring secreted factors (Fig. 8). These observations suggest that the mechanism by which MDSPCs promote healthspan and lifespan extension is likely through the secretion of stimulatory factor(s) that promote regeneration and that cells from old hosts lose this secretory phenotype. This stimulatory factor may be necessary to overcome systemic48 or local58 negative regulators of tissue regeneration in aged hosts. Identification of these secreted factors and methods to rejuvenate adult stem cells ex vivo will be critical for regenerative medicine.
Our data demonstrate that transplanted MDSPCs can have systemic effects, leading to tissue regeneration in multiple organ systems. Therefore, MDSPCs may have therapeutic value for delaying ageing-related functional decline and treating human progerias. It will be of critical importance to test other adult stem cell types for a similar therapeutic effect.
Isolation of MDSPCs
All animal experiments were performed with the approval of the University of Pittsburgh Institutional Animal Care and Use Committee. The fraction of slow-adhering cells referred to as MDSPCs were isolated from the skeletal muscle of mice via a modified preplate technique21,22. Briefly, mouse skeletal muscle is enzymatically digested to a single-cell suspension and the cells are separated by their ability to adhere to collagen-coated flasks. The cells that adhere 2 h post-plating are defined as preplate 1 (pp1, Fig. 2). The non-adherent cells (pp2) are transferred to a fresh flask after 24 h. This is repeated five times to isolate the slowly adhering, stem-cell-enriched MDSPC population (pp6 labelled ft). MDSPCs were isolated from young WT (14–21-day-old; f1 C57BL/6:FVB/n or C57BL/10J), adult WT (19-wk-old; f1), old WT (2-yr-old; f1), progeroid Ercc1−/− (14–21-day-old; f1), Ercc1−/Δ (19-wk-old; f1) and DNA-repair-deficient Xpa−/− (21-day-old; C57BL/6) mice (n=3–4 per strain), which were bred at the University of Pittsburgh Division of Laboratory Animal Research. MDSPCs were cultured in proliferation medium (PM): DMEM supplemented with 10% horse serum, 10% fetal bovine serum (FBS), 1% penicillin–streptomycin (all from Invitrogen), and 0.5% chick embryo extract (Accurate Chemical), on collagen-type I-coated flasks. Primary MEFs used for transplantation were prepared from E13–15 day-old embryos and cultured at 3% O2 (ref. 32).
To isolate individual clones, 5×105 MDSPCs were resuspended in 500 μl media and the FACS Aria cytometer (Becton-Dickinson) was used to place one MDSPC per well in a 96-well collagen-I-coated plate containing 100 μl PM medium. Each well was manually checked to confirm the presence of a single cell. Cells were cultured at 37 °C, 5% CO2, in a humidified incubator and medium was changed every 2 days.
Proliferation in vitro
Cells were plated at a density of 2,000 cells per well in a collagen-type-I–coated 12-well plate. An imaging system (Automated Cell Technologies) equipped with a ×20 objective was used to acquire images at 10-min intervals over a period of 72 h. Forty images at each time point were randomly selected and analysed from three independent experiments on three-to-four distinct cell lines. To estimate PDT, the experimental data sets for each population were curve-fit to the re-arranged Sherley model38,59, using nonlinear regression to determine the correlation coefficient and dividing fraction for each cell genotype.
The division time (DT) of individual cells within the population or of isolated clones was measured by determining the time between cytokinesis events using time-lapsed video recordings (72 h in duration). At least 30 cells were tracked over time in at least 3 MDSPC populations per genotype. For clonal experiments, cells were analysed within 5 days after isolation of clones from pp6. Three-to-four clones were isolated from 2–3 MDSPC populations of each genotype. DT was, therefore, calculated from 6–12 clones per genotype (Supplementary Table S1).
Differentiation in vitro
To induce myogenic differentiation, MDSPCs were plated at high confluence in PM for 2–3 days then shifted to differentiation medium (DM: DMEM, 2% FBS, 1% penicillin–streptomycin). After 2–3 days, immunostaining for f-MyHC, a marker of terminal myogenic differentiation, was performed. After cold methanol fixation, cells were blocked with 5% goat serum and incubated with f-MyHC mouse monoclonal antibody (1:250; Sigma-Aldrich), secondary biotinylated IgG (1:250; Vector Laboratories) for 1 h, and streptavidin-594 (1:500; Sigma-Aldrich) for 15 min to fluorescently label the myotubes. To visualize the nuclei, the cultures were then incubated with DAPI (4′, 6′ diamidino-2-phyenylindole, 100 ng ml−1; Sigma-Aldrich) for 10 min. For negative controls, the primary antibody was omitted or replaced with mouse or rabbit IgG. Fluorescent images were captured and processed using a Leica DMIRB microscope equipped with a QImaging Retiga digital camera both using Northern Eclipse software (v. 6.0; Empix Imaging). We quantified the cells in at least 15 fields each from 3 replica platings of 4 independent experiments. Myogenic differentiation was calculated as the percent of cells (the number of nuclei, detected by DAPI stain) expressing f-MyHC.
For adipogenic, osteogenic, and chondrogenic differentiation, MDSPCs were seeded onto 6-well plates and lineage-specific differentiation media (Lonza) was added for 7 days. Expression of lineage-specific markers was measured by reverse transcriptase PCR (rtPCR) as described in Supplementary Methods and Supplementary Table S5.
The preplate technique was utilized to obtain cells from skeletal muscle21. Red blood cells were eliminated using a lysis buffer before plating. After 24 h, nonadherent cells in pp3 were collected, centrifuged, resuspended in mouse serum (1:10; Sigma-Aldrich) on ice and counted. Cells were then labelled with rat monoclonal antibodies against CD34, Sca-1, and CD45 conjugated to FITC, PE (both from BD Pharmingen), and APC, respectively (Invitrogen). A separate fraction of each cell pellet was stained with equivalent volumes of isotype control antibodies. Before analysis, 7-amino-actinomycin D (7-AAD) was added for exclusion of nonviable cells. Cells were analysed on a FACSAria cytometer using FACS Diva software, (Becton Dickinson). Sorted gates were defined based on separate cell portions that were labelled with equivalent amounts of appropriately conjugated isotype-matched control antibodies. Positive and negative gates were set by analysing isotype or unstained control samples in each analysis. The average percent of Sca-1+/CD34+/CD45− cells was normalized to pellet weight. To compare differentiation potential, an equal number of sorted cells from each group were plated in 48-well plates and differentiation was induced as described above.
Ercc1−/− MDSPCs were plated in the lower compartment of Transwell Permeable Supports (Costar) in PM media at a density of 3,000 cells per well. WT-MDSPCs were seeded into 6.5 mm transwell membrane inserts at the same density in PM media and placed above the ERCC1-deficient MDSPCs. The 24-well plates containing the transwells were then placed in the LCI system for 72 h to acquire time-lapsed images to measure proliferation of the MDSPCs, as described above. As a control, each plate contained wells of Ercc1−/− MDSPCs without transwell membrane inserts. To measure the differentiation potential of Ercc1−/− MDSPCs after co-culture, transwell inserts were removed after 72 h and the PM media was switched to DM. After 2–3 days, myogenic differentiation of the cells was tested by immunostaining for f-MyHC, as described above. To quantify differentiation, it was necessary to switch from transwell to conditioned media from young WT-MDSPCs to generate sufficient cells for quantitative immunodetection of myogenic differentiation markers. Young WT-MDSPCs were cultured for 2 days in 25 cm2 collagen-coated flasks and then treated with differentiation media (DMEM medium supplemented with 2% FBS) for 3 days. The supernatant was collected and used as conditioned media. MDSPCs from 21-day-old Ercc1−/− mice and 2-yr-old WT mice were grown in the presence of this conditioned media or unconditioned differentiation media to determine the impact on myogenic differentiation.
Three-to-four days after induction of myogenic differentiation, cells were lysed in Laemmli Sample Buffer (Bio-Rad), boiled for 5 min, and centrifuged at 4,000 r.p.m. for 5 min. Each sample was loaded on a 6% SDS-polyacrylamide gel, which was run for 2 h by 100 volts and then transferred for 45 min by 20 volts in a semi-dry transferring apparatus. The membrane was blocked with 5% nonfat dry milk (Bio-rad) in Tris-buffered saline Tween-20 for 1 h and then incubated with mouse anti-MyHC (fast) monoclonal antibody (1:500, Sigma-Aldrich) and mouse anti-β-Actin monoclonal antibody (1:8,000, Sigma-Aldrich), respectively, overnight at 4 °C. After washing 3 times with Tris-buffered saline Tween-20, the membrane was incubated with rabbit anti-mouse IgG (H+L) (1:5,000, Pierce) for 1 h at room temperature. Blots were developed by ECL solution (Pierce). Differences in expression were measured by densitometry using software from SCION Corporation (provided by National Institutes of Health) and reported relative to β-Actin.
Microvascular density was measured by co-staining for dystrophin to highlight muscle fibres and CD31 to identify endothelial cells present within microvessels35. Briefly, sections of frozen gastrocnemius muscle were formalin fixed, blocked with serum, and incubated with primary antibodies including CD31 (1:250; BD Biosciences) and dystrophin (1:300, Abcam) for 1 h. Goat anti-rat IgG Alexa Fluor 594 (1:300, Invitrogen) and donkey anti-rabbit Alexa Fluor 488 (1:300, Invitrogen) were used to detect CD31 and dystrophin, respectively. DAPI was used to identify cell nuclei. For each image, the total number of muscle fibres and CD31+ cells were counted, and the ratio of CD31+ cells to muscle fibres was reported35. To detect nLacZ expression, tissue sections were fixed in 1% glutaraldehyde and incubated with 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-gal) solution for 3 h at 37 °C and counterstained with haematoxylin and/or eosin. For details on co-localizing CD31 and nLacZ staining, see Supplementary Methods.
Statistical analyses were carried out using SigmaStat (Jandel Scientific v2.0) software package. The paired Student's t-test or Mann–Whitney rank sum test was used for direct comparisons between treatment and control groups. For multiple comparisons, the one-way ANOVA or the Kruskal–Wallis one-way ANOVA on ranks was applied. Pairwise multiple comparisons were performed using the Tukey's Test or Dunn's Method following rank-based ANOVA. All values are expressed as the mean±s.d. (or s.e.m. where noted), and P<0.05 was considered significant.
How to cite this article: Lavasani, M. et al. Muscle-derived stem/progenitor cell dysfunction limits healthspan and lifespan in a murine progeria model. Nat. Commun. 3:608 doi: 10.1038/ncomms1611 (2012).
Kirkwood, T. B. Understanding the odd science of aging. Cell 120, 437–447 (2005).
Sharpless, N. E. & DePinho, R. A. How stem cells age and why this makes us grow old. Nat. Rev. Mol. Cell Biol. 8, 703–713 (2007).
Rossi, D. J., Jamieson, C. H. & Weissman, I. L. Stems cells and the pathways to aging and cancer. Cell 132, 681–696 (2008).
Molofsky, A. V. et al. Increasing p16INK4a expression decreases forebrain progenitors and neurogenesis during ageing. Nature 443, 448–452 (2006).
Kuhn, H. G., Dickinson-Anson, H. & Gage, F. H. Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation. J. Neurosci. 16, 2027–2033 (1996).
Ryu, B. Y., Orwig, K. E., Oatley, J. M., Avarbock, M. R. & Brinster, R. L. Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal. Stem Cells 24, 1505–1511 (2006).
Zhang, X., Ebata, K. T., Robaire, B. & Nagano, M. C. Aging of male germ line stem cells in mice. Biol. Reprod. 74, 119–124 (2006).
Morrison, S. J., Wandycz, A. M., Akashi, K., Globerson, A. & Weissman, I. L. The aging of hematopoietic stem cells. Nature Med. 2, 1011–1016 (1996).
Fuller, J. Hematopoietic stem cells and aging. Sci. Aging Knowledge Environ. 2002, pe11 (2002).
Conboy, I. M. & Rando, T. A. The regulation of Notch signaling controls satellite cell activation and cell fate determination in postnatal myogenesis. Dev. Cell 3, 397–409 (2002).
Conboy, I. M., Conboy, M. J., Smythe, G. M. & Rando, T. A. Notch-mediated restoration of regenerative potential to aged muscle. Science 302, 1575–1577 (2003).
Shefer, G., Van de Mark, D. P., Richardson, J. B. & Yablonka-Reuveni, Z. Satellite-cell pool size does matter: defining the myogenic potency of aging skeletal muscle. Dev. Biol. 294, 50–66 (2006).
Collins, C. A., Zammit, P. S., Ruiz, A. P., Morgan, J. E. & Partridge, T. A. A population of myogenic stem cells that survives skeletal muscle aging. Stem Cells 25, 885–894 (2007).
Sethe, S., Scutt, A. & Stolzing, A. Aging of mesenchymal stem cells. Ageing Res. Rev. 5, 91–116 (2006).
Stolzing, A., Jones, E., McGonagle, D. & Scutt, A. Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mech. Ageing Dev. 129, 163–173 (2008).
Scaffidi, P. & Misteli, T. Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat. Cell Biol. 10, 452–459 (2008).
Huang, S. C. et al. Mechanical strain modulates age-related changes in the proliferation and differentiation of mouse adipose-derived stromal cells. BMC Cell Biol. 11, 18 (2010).
Grounds, M. D. Age-associated changes in the response of skeletal muscle cells to exercise and regeneration. Ann. N. Y. Acad. Sci. 854, 78–91 (1998).
Welle, S. Cellular and molecular basis of age-related sarcopenia. Can. J. Appl. Physiol. 27, 19–41 (2002).
Thompson, L. V. Age-related muscle dysfunction. Exp. Gerontol. 44, 106–111 (2009).
Qu-Petersen, Z. et al. Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. J. Cell Biol. 157, 851–864 (2002).
Gharaibeh, B. et al. Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique. Nat. Protoc. 3, 1501–1509 (2008).
Peng, H. et al. Synergistic enhancement of bone formation and healing by stem cell-expressed VEGF and bone morphogenetic protein-4. J. Clin. Invest. 110, 751–759 (2002).
Payne, T. R. et al. A relationship between vascular endothelial growth factor, angiogenesis, and cardiac repair after muscle stem cell transplantation into ischemic hearts. J. Am. Coll. Cardiol. 50, 1677–1684 (2007).
Oshima, H. et al. Differential myocardial infarct repair with muscle stem cells compared to myoblasts. Mol. Ther. 12, 1130–1141 (2005).
Crisan, M. et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3, 301–313 (2008).
Shen, J. et al. Transplantation of mesenchymal stem cells from young donors delays aging in mice. Sci. Rep. 1, 67 doi:10.1038/srep00067 (2011).
Sun, Y. et al. Rescuing replication and osteogenesis of aged mesenchymal stem cells by exposure to a young extracellular matrix. FASEB J. 25, 1474–1485 (2011).
Niedernhofer, L. J. et al. A new progeroid syndrome reveals that genotoxic stress suppresses the somatotroph axis. Nature 444, 1038–1043 (2006).
Gregg, S. Q., Robinson, A. R. & Niedernhofer, L. J. Physiological consequences of defects in ERCC1-XPF DNA repair endonuclease. DNA Repair (Amst.) 10, 781–791 (2011).
Weeda, G. et al. Disruption of mouse ERCC1 results in a novel repair syndrome with growth failure, nuclear abnormalities and senescence. Curr. Biol. 7, 427–439 (1997).
Dolle, M. E. et al. Increased genomic instability is not a prerequisite for shortened lifespan in DNA repair deficient mice. Mutat. Res. 596, 22–35 (2006).
Chipchase, M. D., O'Neill, M. & Melton, D. W. Characterization of premature liver polyploidy in DNA repair (Ercc1)-deficient mice. Hepatology 38, 958–966 (2003).
Vo, N. et al. Accelerated aging of intervertebral discs in a mouse model of progeria. J. Orthop. Res. 28, 1600–1607 (2010).
Deasy, B. M. et al. Effect of VEGF on the regenerative capacity of muscle stem cells in dystrophic skeletal muscle. Mol. Ther. 17, 1788–1798 (2009).
Jankowski, R. J., Deasy, B. M. & Huard, J. Muscle-derived stem cells. Gene Ther. 9, 642–647 (2002).
de Vries, A. et al. Increased susceptibility to ultraviolet-B and carcinogens of mice lacking the DNA excision repair gene XPA. Nature 377, 169–173 (1995).
Deasy, B. M. et al. Modeling stem cell population growth: incorporating terms for proliferative heterogeneity. Stem Cells 21, 536–545 (2003).
Hoffman, E. P., Monaco, A. P., Feener, C. C. & Kunkel, L. M. Conservation of the Duchenne muscular dystrophy gene in mice and humans. Science 238, 347–350 (1987).
Mitchell, P. O. et al. Sca-1 negatively regulates proliferation and differentiation of muscle cells. Dev. Biol. 283, 240–252 (2005).
Joe, A. W. et al. Muscle injury activates resident fibro/adipogenic progenitors that facilitate myogenesis. Nat. Cell Biol. 12, 153–163 (2010).
Hidestrand, M. et al. Sca-1-expressing nonmyogenic cells contribute to fibrosis in aged skeletal muscle. J. Gerontol. A Biol. Sci. Med. Sci. 63, 566–579 (2008).
d'Albis, A., Couteaux, R., Janmot, C., Roulet, A. & Mira, J. C. Regeneration after cardiotoxin injury of innervated and denervated slow and fast muscles of mammals. Myosin isoform analysis. Eur. J. Biochem. 174, 103–110 (1988).
Peng, H. et al. VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis. J. Bone Miner. Res. 20, 2017–2027 (2005).
Payne, T. R. et al. Regeneration of dystrophin-expressing myocytes in the mdx heart by skeletal muscle stem cells. Gene Ther. 12, 1264–1274 (2005).
Janzen, V. et al. Stem-cell ageing modified by the cyclin-dependent kinase inhibitor p16INK4a. Nature 443, 421–426 (2006).
Conboy, I. M. et al. Rejuvenation of aged progenitor cells by exposure to a young systemic environment. Nature 433, 760–764 (2005).
Brack, A. S. et al. Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis. Science 317, 807–810 (2007).
Zhang, J. et al. A human iPSC model of Hutchinson Gilford Progeria reveals vascular smooth muscle and mesenchymal stem cell defects. Cell Stem Cell 8, 31–45 (2011).
Baxter, M. A. et al. Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells 22, 675–682 (2004).
Gopinath, S. D. & Rando, T. A. Stem cell review series: aging of the skeletal muscle stem cell niche. Aging Cell 7, 590–598 (2008).
van der Lugt, N. M. et al. Posterior transformation, neurological abnormalities, and severe hematopoietic defects in mice with a targeted deletion of the bmi-1 proto-oncogene. Genes Dev. 8, 757–769 (1994).
Park, I. K. et al. Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature 423, 302–305 (2003).
Nishino, J., Kim, I., Chada, K. & Morrison, S. J. Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf expression. Cell 135, 227–239 (2008).
Nijnik, A. et al. DNA repair is limiting for haematopoietic stem cells during ageing. Nature 447, 686–690 (2007).
Rossi, D. J. et al. Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Nature 447, 725–729 (2007).
Brunet, A. & Rando, T. A. Ageing: from stem to stern. Nature 449, 288–291 (2007).
Carlson, M. E., Hsu, M. & Conboy, I. M. Imbalance between pSmad3 and Notch induces CDK inhibitors in old muscle stem cells. Nature 454, 528–532 (2008).
Sherley, J. L., Stadler, P. B. & Stadler, J. S. A quantitative method for the analysis of mammalian cell proliferation in culture in terms of dividing and non-dividing cells. Cell Prolif. 28, 137–144 (1995).
We thank Ayse Baybars for technical assistance, Minakshi Poddar for performing western blot experiments, Vaishali Patil for maintaining the mouse colony, Alison Logar for assistance with flow cytometry and Dr. Bridget Deasy (University of Pittsburgh, Pittsburgh, PA) for sharing her expertise, methodology and established protocols in regards to the live-cell imaging (LCI) system. This work was supported in part by The Ellison Medical Foundation AG-NS-0303-05 and ES016114, AG033907-01A1, and AR051456 from the National Institutes of Health, the Henry J. Mankin Endowed Chair at the University of Pittsburgh, William F. and Jean W. Donaldson endowed chair at the Children's Hospital of Pittsburgh. J.S.T. was supported by NIH post-doctoral fellowship F30 AG032816. The experiments were approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh.
The authors, except J.H., declare no competing financial interests. J.H. receives consulting fees from Cook MyoSite.
Supplementary Figures S1-S3, Supplementary Tables S1-S5, Supplementary Methods and Supplementary References (PDF 5924 kb)
Time-lapsed movie demonstrating cell proliferation of MDSPCs isolated from progeroid mice. (AVI 6611 kb)
Time-lapsed video demonstrating cell proliferation of MDSPCs isolated from young WT mice. (AVI 9704 kb)
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Lavasani, M., Robinson, A., Lu, A. et al. Muscle-derived stem/progenitor cell dysfunction limits healthspan and lifespan in a murine progeria model. Nat Commun 3, 608 (2012). https://doi.org/10.1038/ncomms1611
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