Crop yield loss due to flooding is a threat to food security. Submergence-induced hypoxia in plants results in stabilization of group VII ETHYLENE RESPONSE FACTORs (ERF-VIIs), which aid survival under these adverse conditions. ERF-VII stability is controlled by the N-end rule pathway, which proposes that ERF-VII N-terminal cysteine oxidation in normoxia enables arginylation followed by proteasomal degradation. The PLANT CYSTEINE OXIDASEs (PCOs) have been identified as catalysts of this oxidation. ERF-VII stabilization in hypoxia presumably arises from reduced PCO activity. We directly demonstrate that PCO dioxygenase activity produces Cys-sulfinic acid at the N terminus of an ERF-VII peptide, which then undergoes efficient arginylation by an arginyl transferase (ATE1). This provides molecular evidence of N-terminal Cys-sulfinic acid formation and arginylation by N-end rule pathway components, and a substrate of ATE1 in plants. The PCOs and ATE1 may be viable intervention targets to stabilize N-end rule substrates, including ERF-VIIs, to enhance submergence tolerance in agriculture.
All aerobic organisms require homeostatic mechanisms to ensure O2 supply and demand are balanced. When supply is reduced (hypoxia), a hypoxic response is required to decrease demand and/or improve supply. In animals, this well-characterized response is mediated by the hypoxia-inducible transcription factor (HIF), which upregulates genes encoding for vascular endothelial growth factor, erythropoietin and glycolytic enzymes among many others1,2,3. Hypoxia in plants is typically a consequence of reduced O2 diffusion under conditions of waterlogging or submergence, or inside of organs such as seeds, embryos or floral meristems in buds where the various external cell layers act as diffusion barriers. Although plants can survive temporary periods of hypoxia, flooding has a negative impact on plant growth and, if sustained, it can result in plant damage or death4. This has a major impact on crop yield; for example, flooding resulted in crop loss costing $3 billion in the United States in 2011 (ref. 5). As climate change results in increased severe weather events including flooding4, strategies to address crop survival under hypoxic stress are needed to meet the needs of a growing worldwide population.
The response to hypoxia in rice, Arabidopsis, and barley is known to be mediated by the group VII ETHYLENE RESPONSE FACTORs (ERF-VIIs)6,7,8,9,10,11. It has been found that these transcription factors promote the expression of core hypoxia-responsive genes, including those encoding alcohol dehydrogenase and pyruvate decarboxylase that facilitate anaerobic metabolism12,13. Crucially, it was shown, initially in Arabidopsis, that the stability of the ERF-VIIs is regulated in an O2-dependent manner via the Arg/Cys branch of the N-end rule pathway, which directs proteins for proteasomal degradation depending on the identity of their amino-terminal amino acid14,15,16. Thus, a connection between O2 availability and the plant hypoxic response was identified11,17,18. The Arabidopsis ERF-VIIs are translated with the conserved N-terminal motif MCGGAI/VSDY/F (ref. 4) and co-translational N-terminal methionine excision, catalysed by Met amino peptidases19,20, leaves an exposed N-terminal Cys, which is susceptible to oxidation14,15,16. N-terminally oxidized Cys residues (Cys-sulfinic acid or Cys-sulfonic acid, Supplementary Fig. 1) are then proposed to render the ERF-VII N termini substrates for arginyl transfer RNA transferase (ATE)-catalysed arginylation. The subsequent Nt-Arg-ERF-VIIs are candidates for ubiquitination by the E3 ligase PROTEOLYSIS6 (PRT6) (ref. 21), which promotes targeted degradation via the 26S proteasome. It has also been shown that degradation of ERF-VIIs by the N-end rule pathway can be influenced by NO, and that the ERF-VIIs play a role in plant NO-mediated stress responses22,23.
The plant hypoxic response mimics the equivalent well-characterized regulatory system in animals, whereby adaptation to hypoxia is mediated by HIF. In normoxic conditions, HIF is hydroxylated at specific prolyl residues targeting it for binding to the von Hippel–Lindau tumour suppressor protein, the recognition component of the E3-ubiquitin ligase complex, which results in HIF ubiquitination and proteasomal degradation1,3. Thus, although not substrates for the N-end rule pathway of protein degradation, HIF levels are regulated by posttranslational modification resulting in ubiquitination, in a manner that is sensitive to hypoxia. HIF prolyl hydroxylation is catalysed by O2-dependent enzymes, the HIF prolyl hydroxylases 1–3 (ref. 2), which are highly sensitive to O2 availability24,25. These O2-sensing enzymes are thus the direct link between O2 availability and the hypoxic response26,27.
Crucially, a family of five enzymes, the PLANT CYSTEINE OXIDASEs (PCO1–5), were identified in Arabidopsis28 that were reported to catalyse the O2-dependent reaction in the plant hypoxic response, specifically the oxidation of the conserved Cys residue at the N terminus of the Arabidopsis ERF-VIIs, RAP2.2, RAP2.12, RAP2.3, HRE1 and HRE2. It was found that overexpression of PCO1 and 2 in planta specifically led to depleted RAP2.12 protein levels and reduced submergence tolerance, whereas pco1 pco2 T-DNA insertion mutants accumulated RAP2.12 protein. Isolated recombinant PCO1 and PCO2 were shown to consume O2 in the presence of pentameric peptides CGGAI corresponding to the N termini of various ERF-VIIs (Supplementary Table 1)28. The identification of these enzymes indicates that the hypoxic response in plants is enzymatically regulated28, potentially in a similar manner to the regulation of the hypoxic response in animals by the HIF hydroxylases. The PCOs may therefore act as plant O2 sensors.
Validation of the chemical steps in the Arg/Cys branch of the N-end rule pathway is still limited, both in animals and plants. We therefore sought to provide molecular evidence that the PCOs catalyse the oxidation step in ERF-VII proteasomal targeting and to determine whether this step is required for further molecular priming by arginylation. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) techniques, we confirm that PCO1 and also PCO4—representatives of the two different PCO ‘subclasses’ based on sequence identity and expression behaviour28—catalyse dioxygenation of the N-terminal Cys of Arabidopsis ERF-VII peptide sequences to Cys-sulfinic acid (CysO2). This oxidation directly incorporates molecular O2. To our knowledge, these are the first described enzymes that catalyse cysteinyl oxidation, as well as being the first described cysteine dioxygenases in plants. We then verify that the Cys-sulfinic acid product of the PCO-catalysed reactions is a direct substrate for the arginyl tRNA transferase ATE1, demonstrating that PCO activity is relevant and sufficient for the subsequent step of molecular recognition and modification according to the N-end rule pathway. This provides the first molecular evidence that Nt-Cys-sulfinic acid is a bona fide substrate for N-end rule-mediated arginylation. Overall, we thus define the PCOs as plant cysteinyl dioxygenases and ATE1 as an active arginyl transferase, establishing for the first time a direct link between molecular O2, PCO catalysis and ATE1 recognition and modification of N-end rule substrates.
PCOs catalyse O2-dependent modification of RAP22–11
N-terminally hexahistidine-tagged recombinant PCO1 and 4 were purified to ∼90% purity, as judged by SDS–polyacrylamide gel electrophoresis (Supplementary Fig. 2a). Protein identity was confirmed by comparison of observed and predicted mass by liquid chromatograpy (LC)–MS (PCO1 predicted mass 36,510 Da, observed mass 36,513 Da; PCO4 predicted mass 30,680 Da, observed mass 30,681 Da, Supplementary Fig. 2b). Both PCO1 and PCO4 were found to be monomeric in solution and to co-purify with substoichiometric levels of Fe(II) (∼0.3 atoms Fe(II) per monomer, Supplementary Fig. 2c–e), in line with the reported parameters of recombinant forms of their distant homologues, the cysteine dioxygenases (CDOs)28,29,30. The activity of the purified PCO1 and PCO4 was tested towards a synthetic 10-mer peptide corresponding to the methionine excised N termini of the ERF-VIIs RAP2.2, RAP2.12 and HRE2 (H2N-CGGAIISDFI-COOH, hereafter termed RAP22–11 Supplementary Table 1). Assays comprising RAP2–11 at 100 μM in the presence or absence of PCO1 or PCO4 at 0.5 μM underwent aerobic or anaerobic coincubation for 30 min at 30 °C before analysis of the peptide by matrix-assisted laser desorption/ionization-MS (MALDI–MS, Fig. 1a,b). Only under aerobic conditions and in the presence of PCO1 or PCO4 did the spectra reveal the appearance of two species with mass increases of +32 Da and +48 Da, corresponding to two or three added O atoms, suggesting an O2-dependent reaction for PCOs 1 and 4 (Fig. 1b), as previously shown for PCOs 1 and 2 (it is noteworthy that supplementation of Fe(II) and/or addition of ascorbate was not required for the endpoint PCO1/4 activity assays conducted in this study)28. These mass shifts were deemed to be consistent with enzymatic formation of Cys-sulfinic (CysO2, +32 Da) and Cys-sulfonic acid (CysO3, +48 Da; Supplementary Fig. 1). Although homology between the PCOs and CDOs28,30 leads to the predisposition that they will perform similar chemistry (that is, catalyse Cys sulfinic acid formation), both Cys-sulfinic and Cys-sulfonic acid are proposed to be Arg transferase substrates in the Arg/Cys branch of N-end rule-mediated protein degradation and therefore both were considered as potential products of the PCO-catalysed reaction14,15,16.
PCOs catalyse dioxygenation of RAP22–11
To ascertain whether the PCOs function as dioxygenases and thus to confirm a direct connection between molecular O2 and PCO activity, we sought to verify the source of the O atoms in the oxidized RAP22–11 by conducting assays in the presence of 18O2 as the cosubstrate or H218O as the solvent. To probe O2 as the source of O atoms in the product, anaerobic solutions of RAP22–11 were prepared in sealed vials before addition of PCO4 using a gas-tight syringe. The vials were then purged with 16O2 or 18O2 and the reactions were allowed to proceed at 30 °C for a subsequent 20 min. Upon analysis by MALDI–MS, the mass of the products revealed that molecular O2 was incorporated into the Cys-sulfinic acid product (Fig. 2a). The Cys-sulfinic acid product had a mass of +32 Da in the presence of 16O2 and +36 Da in the presence of 18O2, demonstrating addition of two 18O atoms and indicating that O2 is the source of O atoms in this product. The Cys-sulfonic acid product had a mass of +52 Da in the presence of 18O2, indicating a third 18O atom had not been incorporated into this product. To probe whether the source of the additional mass in the apparent Cys-sulfonic acid product was an O atom derived from water, an equivalent reaction was carried out under aerobic conditions in the presence of H218O (H218O:H2O in a 3:1 ratio). No additional mass was observed in the peak corresponding to the Cys-sulfonic acid, raising the possibility that the +48 Da species observed by MALDI–MS is not enzymatically formed. Importantly, following incubation in the presence of H218O, no additional mass was observed in the peak corresponding to Cys-sulfinic acid, confirming that this species is a product of a reaction where molecular O2 is a substrate (Fig. 2b).
To further investigate whether the PCO-catalysed product species observed at +48 Da is enzymatically produced or an artefact of the MALDI–MS analysis method, we turned to LC–MS, to analyse the products of the PCO-catalysed reactions. Under these conditions, only peptidic product with a mass increase of +32 Da was observed after incubation with both PCO1 and PCO4, corresponding to the incorporation of two O atoms and the formation of Cys-sulfinic acid (Fig. 2c), consistent with the products observed using 18O2 and H218O (Fig. 2a,b). No product was observed with a mass corresponding to Cys-sulfonic acid, which suggested that the +48 Da product detected by MALDI–MS was indeed an artefact. When combined with the observation that significant quantities of Cys-sulfonic acid were not seen in the no-enzyme or anaerobic controls (Fig. 1), it was hypothesized that the Cys-sulfinic acid product of the PCO-catalysed reaction is non-enzymatically converted to Cys-sulfonic acid during MALDI–MS analysis, potentially as a result of laser exposure. Upon subjecting the products of PCO1 and 4 turnover to MALDI–MS analysis with increasing laser intensity, a direct correlation between laser intensity and the ratio of Cys-sulfonic acid:Cys-sulfinic acid product was observed (Supplementary Fig. 3a). Of note, significant levels of laser induced formation of +32 and +48 Da species upon analysis of unmodified peptide were not observed (Supplementary Fig. 3b). Together, these results confirm that the +48 Da species observed following incubation of the PCOs with RAP22–11 are a product of Cys-sulfinic acid exposure to the MALDI–MS laser and not a product of the PCO-catalysed reaction. Overall, these data demonstrate that the PCOs are dioxygenase enzymes, similar to the mammalian and bacterial CDOs to which they show sequence homology28,30.
PCOs catalyse RAP22–11 N-terminal Cys-sulfinic acid formation
Recombinant PCO1 and PCO2 were reported to consume O2 in the presence of pentameric CGGAI peptides corresponding to the methionine-excised N terminus of the Arabidopsis ERF-VIIs28. To definitively verify that the N-terminal cysteinyl residue of RAP22–11 is indeed the target for the PCO-catalysed +32 Da modifications, we conducted LC–MS/MS analyses on the reaction products. Fragmentation of RAP22–11 that had been incubated in the presence and absence of PCO1 and PCO4 revealed b- and y-ion series consistent with oxidation of the N-terminal Cys residue (Fig. 3a), confirming that PCOs 1 and 4 act as cysteinyl dioxygenases.
As a final confirmation of the nature of the reaction catalysed by PCO1 and PCO4, their activity was monitored using 1H-NMR. Reactions were initiated by adding 5 μM enzyme to 500 μM RAP22–11 (in the presence of 10% D2O) and products of the reaction were analysed using a 600 MHz NMR spectrometer. In the presence of both PCO1 and PCO4, modification to the cysteinyl residues was observed, as exemplified by the disappearance of the 1H-resonance corresponding to the β-cysteinyl protons (at δH 2.88 p.p.m.) and the emergence of a new 1H-resonance at δH 2.67 p.p.m. (Fig. 3b). The chemical shift of the new resonance is similar to that observed for L-Cys conversion to L-Cys-sulfinic acid by mouse CDO (ref. 31) and also to the chemical shift of an L-Cys-sulfinic acid standard measured under equivalent conditions to the PCO assays (Supplementary Fig. 4). Therefore, the resonance shift observed upon PCO1/4 reaction was assigned to the β-protons of L-Cys-sulfinic acid. Overall, these results provide confirmation at the molecular level that Arabidopsis PCOs 1 and 4 act as plant cysteinyl dioxygenases, catalysing incorporation of O2 into N-terminal Cys residues on a RAP2 peptide to form Cys-sulfinic acid.
ATE1 arginylates acidic N termini including Cys-sulfinic acid
We next sought to confirm that the PCO-catalysed Cys-oxidation to Cys-sulfinic acid renders a RAP2 peptide capable of and sufficient for onward modification by ATE1. Cys-sulfinic acid has been proposed as a substrate for ATE1 on the basis of its structural homology with known ATE1 substrates Asp and Glu, but evidence has only been reported to date for arginylation of Cys-sulfonic acid32,33. We further sought to validate the role of a plant ATE1: to date ATE1 has been suggested to be responsible for transfer of 3H-arginine to bovine α-lactalbumin in highly purified plant extracts in vitro34 and RAP2.12 stabilization in ate1 ate2 double-null mutant plant lines implicates ATE1 as an ERF-VII-targeting arginyl transferase in vivo17,18. To this end, we produced recombinant hexahistidine-tagged Arabidopsis ATE1 (Supplementary Fig. 5) for use in an arginylation assay, which detects incorporation of radiolabelled 14C-Arg into biotinylated peptides. Carboxy-terminally biotinylated RAP22–13 peptides (H2N-XGGAIISDFIPP(PEG)K(biotin)-NH2) where the N-terminal residue, X, constitutes Gly, Asp, Cys or Cys-sulfonic acid were subjected to the arginylation assay in the presence or absence of PCO1/4 (Fig. 4a). Peptide with an N-terminal Gly did not accept Arg, whereas an N-terminal Asp did accept Arg, independent of the presence of PCO1 or 4. A peptide comprising an N-terminal Cys-sulfonic acid was also shown to be a substrate for ATE1, again independent of the presence of PCO1 or 4, which is in line with proposed steps of the Arg/Cys N-end rule pathway and has also recently been reported using a similar assay with mouse ATE1 (refs 14, 15, 16, 35). Crucially, in the absence of PCO1/4, RAP22–13 with an N-terminal Cys was not an acceptor of arginine transfer by ATE1, yet when either PCO1 or PCO4 was incorporated in the reaction, significant ATE1 transferase activity was observed (Fig. 4a).
To confirm that the increased detection of radiolabelled arginine corresponded to arginyl incorporation at the N termini of the peptides, the experiment was repeated using non-radiolabelled arginine in the presence and absence of PCO4 and ATE1, and peptide products subjected to LC–MS analysis (Fig. 4c). As with RAP22–11 (Fig. 2c), the Cys-initiated RAP22–13 peptide displayed a +32 Da increase in mass upon incubation with PCO4 only (Fig. 4c, red spectrum). Importantly, following incubation of Cys-initiated RAP22–13 with both PCO4 and ATE1, a mass increase equivalent to oxidation coupled to arginylation (+188 Da) was observed (Fig. 4c, blue spectrum). Subsequent tandem MS analysis of these product ions revealed fragmentation species consistent with the assumption that oxidation and sequential arginylation occur at the N terminus of PCO4- and ATE1-treated peptides (Fig. 4d, blue spectrum), strongly suggesting that the PCO-oxidized N termini of ERF-VIIs are rendered N-degrons via additional arginylation (Fig. 4b).
A +12 Da mass increase was observed in control assays lacking PCO4 (Fig. 4c,d; purple spectra). This appeared to be related to prolonged incubation in the presence of HEPES and dithiothreitol (DTT) as used in the arginylation assay buffer: The +12 Da modification was not observed if the peptide was dissolved in H2O (Fig. 4c, black spectrum) or if incubated with HEPES and DTT for just 1 h, but was observed when the peptide was incubated with HEPES and DTT overnight (Supplementary Fig. 6). It is proposed that under these conditions, trace levels of contaminating formaldehyde react with free Nt-Cys residues to form thiazolidine N termini36.
These results are in line with proposed arginylation requirements for the Arg/Cys branch of the N-end rule pathway14,15,16 including the known Cys-initiated arginylation targets from mammals32,33,35,37. Importantly, these results demonstrate for the first time Arg transfer mediated by a plant ATE dependent on the N-terminal residue of its substrate, and also that both Cys-sulfinic acid (the product of PCO-catalysis) and Cys-sulfonic acid can act as substrates for ATE1. In particular, the arginylation observed with PCO-catalysed Cys-sulfinic acid supports the assumption that N-terminal residues sterically and electrostatically resembling Asp or Glu can serve as Arg acceptors in reactions catalysed by ATEs33, and also confirms the importance of the PCOs as a connection between the stability of their ERF-VII substrates and O2 availability (Fig. 4b).
The PCOs were identified in Arabidopsis as a set of five enzymes suggested to catalyse oxidation of N-terminal cysteine residues in ERF-VII transcription factors and oxygen consumption was demonstrated for reactions with short peptides corresponding to their N termini28. This putative oxidation was associated with destabilization of the ERF-VIIs, presumably by rendering them substrates of the Arg/Cys branch of the N-end rule pathway14,16. Under conditions of sufficient O2 availability ERF-VII protein levels are decreased, whereas under hypoxic conditions, such as those encountered upon plant submergence or in the context of organ development, ERF-VII levels remain high17,18. Importantly, the ERF-VII transcription factors are known to upregulate genes which allow plants to cope with or respond to submergence13. The PCOs are proposed to act as potential O2 sensors involved in regulating the plant hypoxic response28.
We sought to biochemically confirm the role of the PCOs in the plant hypoxic response, and present here MS and NMR data that clearly demonstrate that two enzymes from different ‘subclasses’ of this family, PCOs 1 and 4, are dioxygenases that catalyse direct incorporation of O2 into RAP22–11 peptides to form Cys-sulfinic acid. Their direct use of O2 supports the proposal that these enzymes may act as plant O2 sensors28. A relationship has been demonstrated between O2 concentration and PCO activity28, but it will be of interest to perform detailed kinetic characterization of these enzymes to ascertain their level of sensitivity to O2 availability, in particular to determine whether their O2-sensitivity is similar to that of the HIF hydroxylases in animals24,25. Although there is functional homology between the PCOs and the HIF hydroxylases, they are apparently mechanistically divergent: the PCOs show sequence homology to the Fe(II)-dependent CDO family of enzymes, which do not require an external electron donor for O2 activation28,30, whereas the HIF hydroxylases are Fe(II)/2OG-dependent oxygenases. They also co-purified with Fe(II) as reported for both the CDOs29 and prolyl hydroxylase 2 (ref. 38). Of note, the PCOs are the first identified CDOs in plants. Further, in contrast to the reactions of mammalian and bacterial CDOs, which oxidize free L-Cys, the PCOs are also, to our knowledge, the first identified cysteinyl (as opposed to free L-Cys) dioxygenases.
According to the Arg/Cys branch of the N-end rule pathway, N-terminal Cys oxidation is proposed to enable successive arginylation by ATE1 to render proteins as N-degrons. Although both Cys-sulfinic and Cys-sulfonic acid are repeatedly reported as potential arginylation substrates14,15,16, detailed evidence has only been presented to date for arginylation of Cys-sulfonic acid32,33 and this only in a mammalian system. We therefore sought to demonstrate that PCO-catalysed ERF-VII N-terminal Cys oxidation to Cys-sulfinic acid promotes arginylation by ATE1. The arginylation assay and MS results we present demonstrate that the PCO-catalysed dioxygenation reaction is sufficient to trigger N-terminal arginylation of ERF-VIIs by ATE1, thus probably rendering N-termini of ERF-VIIs (at least those comprising the tested N-terminal sequence) as N-degrons, that is, allowing recognition by PRT6 and other potential E3 ubiquitin ligases, polyubiquitination and possibly transfer to the 26S proteasome for proteolysis14,15,16. Collectively, therefore, we present the comprehensive molecular evidence confirming the Cys oxidation and subsequent arginylation steps of the Arg/Cys branch of the N-end rule pathway32,33,37. We also confirm that ATE1 is able to selectively arginylate, as predicted33, acidic N-terminal residues of plant substrates, including Cys-sulfonic acid.
Arginylation has been known as a post-translational modification since 1963 (ref. 39), to possess a general aminoacyl transferase function in plants (rice and wheat) since 1973 (ref. 40) and to have a speculative involvement in the N-end rule pathway since 1988 (refs 41, 42). ATE1 is reported as being capable of arginylating proteins at both acidic N termini and midchain acidic side chains via canonical and non-canonical peptide bonds, respectively43. Reports of midchain arginylation highlighted a potentially broad involvement of ATEs in posttranslational protein modifications for various functions35,43,44,45 but was only very recently brought into question by 14C-Arg incorporation assays using arrays of immobilized synthetic peptides35,43,44. However, to date only one physiological and two in vitro substrates for the Arg/Cys branch of the N-end rule pathway have been characterized, namely mammalian regulator of G-protein signalling (RGS) 4, and RGS5 and 10, respectively46, where Nt-Cys oxidation was described (to Cys-sulfonic acid) as was Nt-Cys arginylation33,37. The first non Cys-branch N-end rule arginylation target was shown to require posttranslational proteolytic cleavage of a (pre-)-proprotein. The C-terminal fragment of proteolytically cleaved mouse BRCA1 is Asp-initiated47 and gets degraded in an N-end rule-dependent manner. Then, the molecular chaperone BiP (GRP78 and HSPA5, heat shock 70 kDa protein 5) and the oxidoreductase protein disulphide isomerase, present Glu or Asp after cleavage of their signal peptide, respectively, and were suggested but not shown as putative N-end rule substrates48. Only very recently, BiP and protein disulphide isomerase were identified in mammalian cell culture together with the Glu-initiated calreticulin as arginylation targets with a function in autophagy rather than the N-end rule degradation49.
Similarly, data regarding the molecular requirements of plant ATEs are limited. Already in 1973, a general aminoacyl transfer activity was found in rice and wheat cell extracts, however, the nature of enzyme, acceptor position and mechanism remained unclear. It was suggested that the N-terminus could serve as Arg acceptor40.
The first description of a mutant of the single translatable ATE1 gene in the Arabidopsis accession Wassilewskija (Ws-0) highlighted a role of ATEs in plant development. Ws-0 lacks the second bona fide ATE, that is, ATE2, due to a single-nucleotide polymorphism in ATE2 causing a premature stop50. Developmental functions of the single homologue ATE1 in the moss Physcomitrella patens were recently described51. Interaction partners of the enzyme were found as well as four arginylated peptides immunologically detected by using antibodies directed against peptides mimicking N-terminal Arg-Asp or Arg-Glu52. In one case, that is the acylamino-acid-releasing enzyme PpAARE, which presents for unknown reasons a neo-N-terminal Asp residue, which was formerly Asp2 and therefore initiated by Met, an N-terminal arginylation was found with high confidence. Previously, Arg transferase function of Arabidopsis ATE1/2 has been shown using an assay detecting conjugation of 3H-Arg to bovine α-lactalbumin (bearing an N-terminal Glu) in the presence of plant extracts from wild-type Arabidopsis, and ate1 and ate2 single mutants but not from ate1 ate2 double mutant seedlings34. Therefore, the results we present here demonstrate for the first time Arg transferase activity of a plant ATE towards known plant N-end rule substrates.
Interestingly, in combination with O2, nitric oxide was identified as an RGS-oxidizing agent, suggesting a potential role of S-nitrosylation in the Arg/Cys branch of the N-end rule pathway, albeit non-enzymatically controlled32. It has also been reported in planta that both NO and O2 are required for ERF-VII degradation, potentially at the Cys oxidation step22,23. Although in N-end rule-mediated RGS4/5 degradation it has been proposed that Cys nitrosylation precedes Cys oxidation (also currently considered a non-enzymatic process), we find that under the conditions used, the PCO1/4-catalysed reaction does not require either prior Cys nitrosylation or exogenous NO to proceed efficiently. We cannot rule out that NO plays a role in formation of a Cys-sulfonic acid product, which is also a substrate for ATE1 as shown in our Arg transfer experiments. Alternatively, NO may have a role in ERF-VII degradation in vivo via non-enzymatic oxidation or via a secondary mechanism. The manner in which NO contributes to Arg/Cys branch of the N-end rule pathway therefore remains to be elucidated.
ERF-VII stabilization has been shown to result in improved submergence tolerance, elegantly demonstrated in barley by mutation of the candidate E3-ubiquitin ligase PRT6 (ref. 11), but also in rice containing the Sub1A gene; SUB1A is an apparently stable ERF-VII that confers particular flood tolerance in certain rare varieties of rice9,17. Overexpression of Sub1A in more commonly grown rice varieties has resulted in a 45% increase in yield relative to sub1a mutant lines after exposure to flooding53. If ERF-VII stabilization is indeed a proficient mechanism for enhancing flood tolerance, then manipulation of PCO or ATE activity may be an efficient and effective point of intervention. This work presents molecular validation of their function, providing the basis for future targeted chemical/genetic inhibition of their activity. It also highlights genetic strategies for breeding via introgression of variants of N-end rule pathway components or introduction of alleles of enzymatic components of the N-end rule pathway from non-crop species into crops. Any of these strategies has the potential to result in stabilized ERF-VII levels and increase stress resistance, and may therefore help to address food security challenges.
All reagents used were purchased from Sigma-Aldrich unless otherwise stated. The 10-mer RAP22–11 peptide (H2N-CGGAIISDFI-COOH) was purchased from GL Biochem (Shanghai) Ltd, China (Supplementary Table 1). The sequence of the 12-mer RAP22–13 peptides used in the coupled oxidation-arginylation assay is derived from RAP2.2, RAP2.12 and HRE2 (H2N-X-GGAIISDFIPP(PEG)K(biotin)-NH2), and synthesized by Fmoc-based solid-phase peptide synthesis on NovaSyn TGR resin (Merck KGaA, Supplementary Table 2). Fmoc protected amino acids (Iris Biotech GmbH) were coupled using 4 equivalents (eq) of the amino acid according to the initial loading of the resin. 4 eq amino acid was mixed with 4 eq O-(6-chlorobenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate and 8 eq N,N-diisopropylethylamine (Santa Cruz Biotechnology, sc-293894), and added to the resin for 1 h. In a second coupling, the resin was treated with 4 eq of the Fmoc-protected amino acid mixed with 4 eq benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluoro-phosphate and 8 eq 4-methylmorpholine for 1 h. After double coupling a capping step to block free amines was performed using acetanhydride and N,N-diisopropylethylamine in N-methyl-2-pyrrolidinone (1:1:10) for 5 min. The C-terminal Fmoc-Lys(biotin)-OH, the 8-(9-fluorenylmethyloxycarbonyl-amino)-3.6-dioxaoctanoic acid (PEG) linker and the different Fmoc protected N-terminal amino acids were coupled manually. The remaining peptide sequence was assembled using an automated synthesizer (Syro II, MultiSynTech GmbH). Fmoc deprotection was performed using 20% piperidine in dimethylformamide (DMF) for 5 min, twice. After each step the resin was washed five times with DMF, methylene chloride (DCM) and DMF, respectively. Final cleavage was performed with 94% trifluoroacetic acid (TFA), 2.5% 1,2-ethanedithiole and 1% triisopropylsilane in aqueous solution for 2 h, twice. The cleavage solutions were combined and peptides were precipitated with diethyl ether (Et2O) at −20 °C for 30 min. Peptides were solved in water/acetonitrile (ACN) 7:3 and purified by reversed-phase HPLC (Nucleodur C18 culumn; 10 × 125 mm, 110 Å, 5 μm particle size; Macherey-Nagel) using a flow rate of 6 ml min−1 (A: ACN with 0.1% TFA, B: water with 0.1% TFA). Obtained pure fractions were pooled and lyophilized. Peptide characterization was performed by analytical HPLC (1260 Infinity, Agilent Technology; flow rate of 1 ml min−1, A: ACN with 1% TFA, B: water with 1% TFA) coupled with a mass spectrometer (6120 Quadrupole LC–MS, Agilent Technology) using electrospray ionization (Agilent Eclipse XDB-C18 culumn, 4.6 × 150 mm, 5 μm particle size). Analytical HPLC chromatograms were recorded at 210 nm (Supplementary Fig. 7). Quantification was performed by HPLC-based comparison (chromatogram at 210 nm) with a reference peptide (Supplementary Table 2).
Protein expression and purification
Arabidopsis PCO1 and PCO4 sequences in pDEST17 bacterial expression vectors (Invitrogen) were kindly provided by F. Licausi and J. van Dongen28. Plasmids were transformed into BL21(DE3) Escherichia coli cells and expression of recombinant protein carrying an N-terminal hexahistidine tag was induced with 0.5 mM isopropyl-β-D-thiogalactoside and subsequent growth at 18 °C for 18 h. Harvested cells were lysed by sonication and proteins purified using Ni++ affinity chromatography, before buffer exchange into 250 mM NaCl/50 mM Tris-HCl (pH 7.5). Analysis by SDS–PAGE and denaturing LC–MS showed proteins with >90% purity and with the predicted molecular weights.
The coding sequence of Arabidopsis ATE1 was cloned according to gene annotations at TAIR (www.arabidopsis.org) from complementary DNA. The sequence was flanked by an N-terminal tobacco etch virus recognition sequence for facilitated downstream purification (‘tev’: ENLYFQ-X) using the primers ate1_tev_ss (5′-GCTTAGAGAATCTTTATTTTCAGGGGATGTCTTTGAAAAACGATGCGAGT-3′) and ate1_as (5′-GGGGACCACTTTGTACAAGAAAGCTGGGTATCAGTTGATTTCATACACCATTCTCTC-3′). A second PCR using the primers adapter (5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAGAGAATCTTTATTTTCAGGGG-3′) and ate1_as was performed to amplify the construct to use it in a BP reaction for cloning into pDONR201 (Invitrogen) followed by an LR reaction into the vector pDEST17 (Invitrogen). The N-terminal hexahistidine fusion was expressed in BL21-CodonPlus (DE3)-RIL E. coli cells. The expression culture was induced with 1 mM isopropyl-β-D-thiogalactoside at optical density 0.6 and grown for 16 h at 18 °C. After resuspension in LEW buffer (50 mM NaH2PO4 pH 8, 300 mM NaCl and 1 mM DTT), the cells were lysed by incubation with 1.2 mg ml−1 lysozyme for 30 min and underwent subsequent sonification in the presence of 1 mM phenylmethylsulfonyl fluoride. Recombinant protein was purified by Ni++ affinity chromatography and subjected to Amicon Ultra-15 (30 K) (Merck Millipore) filtration for buffer exchange to imidazole-free LEW containing 20% glycerol.
PCO activity assays and MALDI analysis
PCO activity assays were conducted under the following conditions, unless otherwise stated: PCO1 or 4 (1 μM) was mixed with 100 or 200 μM RAP22–11 peptide in 250 mM NaCl, 1 mM DTT, 50 mM Tris-HCl pH 7.5 and incubated at 30 °C for 30–60 min. Addition of exogenous Fe(II) and/or ascorbate were not required for activity. Assays were stopped by quenching 1 μl sample with 1 μl α-cyano-4-hydroxycinnamic acid matrix on a MALDI plate before product mass analysis using a Sciex 4800 TOF/TOF mass spectrometer (Applied Biosystems) operated in negative ion reflectron mode. The instrument parameters and data acquisition were controlled by 4000 Series Explorer software and data processing was completed using Data Explorer (Applied Biosystems).
To test the activity of PCO4 in the presence of 18O2, 100 μl of an anaerobic solution of 100 μM RAP22–11 in 250 mM NaCl/50 mM Tris-HCl pH 7.5 was prepared in a septum-sealed glass vial by purging with 100% N2 for 10 min at 100 ml min−1 using a mass flow controller (Brooks Instruments), as used for previous preparation of anaerobic samples to determine enzyme dependence on O2 (ref. 24). PCO4 was then added using a gas-tight Hamilton syringe, followed by purging with a balloon (∼0.7 l) of 16O2 or 18O2 over the course of 10 min at room temperature. Reaction vials were then transferred to 30 °C for a further 20 min before products were analysed by MALDI–MS as described above.
PCO4 activity was additionally tested in the presence of H218O by conducting an assay in 75% H218O, 25% H2O (with all enzyme/substrate/buffer components comprising a portion of the H2O fraction). Assays were conducted for 10 min at room temperature followed by 20 min at 30 °C for comparison with assays conducted with 18O2. Products were analysed by MALDI–MS, as described above.
UPLC–MS and MS/MS analysis
Ultra-high performance chromatography (UPLC)–MS measurements were obtained using an Acquity UPLC system coupled to a Xevo G2-S Q-ToF mass spectrometer (Waters) operated in positive electrospray mode. Instrument parameters, data acquisition and data processing were controlled by Masslynx 4.1. Source conditions were adjusted to maximize sensitivity and minimize fragmentation while Lockspray was employed during analysis to maintain mass accuracy. Two microlitres of each sample was injected on to a Chromolith Performance RP-18e 100-2 mm column (Merck) heated to 40 °C and eluted using a gradient of 95% deionized water supplemented with 0.1% (v/v) formic acid (analytical grade) to 95% acetonitrile (HPLC grade) and a flow rate of 0.3 ml min−1. Fragmentation spectra of substrate and product peptide ions (MS/MS) were obtained using a targeted approach with a typical collision-induced dissociation energy ramp of 30 to 40 eV. Analysis was carried out with the same source settings, flow rate and column elution conditions as above.
Reaction components (5 μM PCO1 or PCO4 and 500 μM RAP22–11) were prepared to 75 μl in 156 mM NaCl, 31 mM Tris-HCl (pH 7.5) and 10% D2O (enzyme added last), in a 1.5 ml microcentrifuge tube before being transferred to a 2 mm diameter NMR tube. 1H-NMR spectra at 310 K were recorded using a Bruker AVIII 600 (with inverse cryoprobe optimized for 1H observation and running topspin 2 software; Bruker) and reported in p.p.m. relative to D2O (δH 4.72). The deuterium signal was also used as internal lock signal and the solvent signal was suppressed by presaturating its resonance.
The conditions for arginylation of the 12-mer peptide substrates were modified from ref. 43. In detail, ATE1 was incubated at 10 μM in the reaction mixture containing 50 mM HEPES pH 7.5, 25 mM KCl, 15 mM MgCl2, 1 mM DTT, 2.5 mM ATP; 0.6 mg ml−1 E. coli tRNA (R1753, Sigma), 0.04 mg ml−1 E. coli aminoacyl-tRNA synthetase (A3646, Sigma), 80 μM (4 nCi μl−1) 14C-arginine (MC1243, Hartmann Analytic), 50 μM C-terminally biotinylated 12-mer peptide substrate and, where indicated, 1 μM purified recombinant PCO1 or PCO4 in a total reaction volume of 50 μl. The reaction was conducted at 30 °C for 16–40 h. After incubation, each 50 μl of avidin agarose bead slurry (20219, Pierce) equilibrated in PBSN (PBS-Nonidet; 100 mM NaH2PO4, 150 mM NaCl; 0.1% Nonidet-P40) was added to the samples and mixed with an additional 350 μl of PBSN. After 2 h of rotation at room temperature, the beads were washed four times in PBSN, resuspended in 4 ml of FilterSafe scintillation solution (Zinsser Analytic) and scintillation counting was performed using a Beckmann Coulter LS 6500 Multi-Purpose scintillation counter.
The authors declare that all data supporting the findings of this study are available within the manuscript and its Supplementary Information files or are available from the corresponding authors upon request.
How to cite this article: White, M. D. et al. Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets. Nat. Commun. 8, 14690 doi: 10.1038/ncomms14690 (2017).
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Kaelin, W. G. Jr & Ratcliffe, P. J. Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol. Cell 30, 393–402 (2008).
Myllyharju, J. Prolyl 4-hydroxylases, master regulators of the hypoxia response. Acta Physiol. (Oxf) 208, 148–165 (2013).
Semenza, G. L. Oxygen sensing, homeostasis, and disease. N. Engl. J. Med. 365, 537–547 (2011).
Bailey-Serres, J. et al. Making sense of low oxygen sensing. Trends Plant Sci. 17, 129–138 (2012).
Bailey-Serres, J., Lee, S. C. & Brinton, E. Waterproofing crops: effective flooding survival strategies. Plant Physiol. 160, 1698–1709 (2012).
Hattori, Y. et al. The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature 460, 1026–1030 (2009).
Hinz, M. et al. Arabidopsis RAP2.2: an ethylene response transcription factor that is important for hypoxia survival. Plant Physiol. 153, 757–772 (2010).
Licausi, F. et al. HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana. Plant J. 62, 302–315 (2010).
Xu, K. et al. Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature 442, 705–708 (2006).
Papdi, C. et al. Functional identification of Arabidopsis stress regulatory genes using the controlled cDNA overexpression system. Plant Physiol. 147, 528–542 (2008).
Mendiondo, G. M. et al. Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6. Plant Biotechnol. J. 14, 40–50 (2016).
Lee, S. C. et al. Molecular characterization of the submergence response of the Arabidopsis thaliana ecotype Columbia. New Phytol. 190, 457–471 (2011).
Mustroph, A. et al. Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. Proc. Natl Acad. Sci. USA 106, 18843–18848 (2009).
Varshavsky, A. The N-end rule pathway and regulation by proteolysis. Protein Sci. 20, 1298–1345 (2011).
Tasaki, T., Sriram, S. M., Park, K. S. & Kwon, Y. T. The N-end rule pathway. Annu. Rev. Biochem. 81, 261–289 (2012).
Gibbs, D. J., Bacardit, J., Bachmair, A. & Holdsworth, M. J. The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions. Trends Cell Biol. 24, 603–611 (2014).
Gibbs, D. J. et al. Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants. Nature 479, 415–418 (2011).
Licausi, F. et al. Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization. Nature 479, 419–422 (2011).
Ross, S., Giglione, C., Pierre, M., Espagne, C. & Meinnel, T. Functional and developmental impact of cytosolic protein N-terminal methionine excision in Arabidopsis. Plant Physiol. 137, 623–637 (2005).
Giglione, C., Boularot, A. & Meinnel, T. Protein N-terminal methionine excision. Cell Mol. Life Sci. 61, 1455–1474 (2004).
Garzon, M. et al. PRT6/At5g02310 encodes an Arabidopsis ubiquitin ligase of the N-end rule pathway with arginine specificity and is not the CER3 locus. FEBS Lett. 581, 3189–3196 (2007).
Gibbs, D. J. et al. Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors. Mol. Cell 53, 369–379 (2014).
Gibbs, D. J. et al. Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants. Plant Physiol. 169, 23–31 (2015).
Tarhonskaya, H. et al. Investigating the contribution of the active site environment to the slow reaction of hypoxia-inducible factor prolyl hydroxylase domain 2 with oxygen. Biochem. J. 463, 363–372 (2014).
Hirsila, M., Koivunen, P., Gunzler, V., Kivirikko, K. I. & Myllyharju, J. Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor. J. Biol. Chem. 278, 30772–30780 (2003).
Bruick, R. K. Oxygen sensing in the hypoxic response pathway: regulation of the hypoxia-inducible transcription factor. Genes Dev. 17, 2614–2623 (2003).
Epstein, A. C. et al. C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation. Cell 107, 43–54 (2001).
Weits, D. A. et al. Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway. Nat. Commun. 5, 3425 (2014).
Imsand, E. M., Njeri, C. W. & Ellis, H. R. Addition of an external electron donor to in vitro assays of cysteine dioxygenase precludes the need for exogenous iron. Arch. Biochem. Biophys. 521, 10–17 (2012).
Joseph, C. A. & Maroney, M. J. Cysteine dioxygenase: structure and mechanism. Chem. Commun. (Camb) 32, 3338–3349 (2007).
Li, W. & Pierce, B. S. Steady-state substrate specificity and O2-coupling efficiency of mouse cysteine dioxygenase. Arch. Biochem. Biophys. 565, 49–56 (2015).
Hu, R. G. et al. The N-end rule pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature 437, 981–986 (2005).
Kwon, Y. T. et al. An essential role of N-terminal arginylation in cardiovascular development. Science 297, 96–99 (2002).
Graciet, E. et al. The N-end rule pathway controls multiple functions during Arabidopsis shoot and leaf development. Proc. Natl Acad. Sci. USA 106, 13618–13623 (2009).
Wadas, B., Piatkov, K. I., Brower, C. S. & Varshavsky, A. Analyzing N-terminal arginylation through the use of peptide arrays and degradation assays. J. Biol. Chem. 291, 20976–20992 (2016).
Kallen, R. G. The mechanism of reactions involving Schiff base intermediates. Thiazolidine formation from L-cysteine and formaldehyde. J. Am. Chem. Soc. 93, 6236–6248 (1971).
Davydov, I. V. & Varshavsky, A. RGS4 is arginylated and degraded by the N-end rule pathway in vitro. J. Biol. Chem. 275, 22931–22941 (2000).
McNeill, L. A. et al. Hypoxia-inducible factor prolyl hydroxylase 2 has a high affinity for ferrous iron and 2-oxoglutarate. Mol. Biosyst. 1, 321–324 (2005).
Kaji, H., Novelli, G. D. & Kaji, A. A soluble amino acid-incorporating system from rat liver. Biochim. Biophys. Acta 76, 474–477 (1963).
Manahan, C. O. & App, A. A. An arginyl-transfer ribonucleic acid protein transferase from cereal embryos. Plant Physiol. 52, 13–16 (1973).
Ciechanover, A. et al. Purification and characterization of arginyl-tRNA-protein transferase from rabbit reticulocytes. Its involvement in post-translational modification and degradation of acidic NH2 termini substrates of the ubiquitin pathway. J. Biol. Chem. 263, 11155–11167 (1988).
Bohley, P., Kopitz, J. & Adam, G. Surface hydrophobicity, arginylation and degradation of cytosol proteins from rat hepatocytes. Biol. Chem. Hoppe Seyler 369, 307–310 (1988).
Wang, J. et al. Arginyltransferase ATE1 catalyzes midchain arginylation of proteins at side chain carboxylates in vivo. Chem. Biol. 21, 331–337 (2014).
Eriste, E. et al. A novel form of neurotensin post-translationally modified by arginylation. J. Biol. Chem. 280, 35089–35097 (2005).
Wong, C. C. et al. Global analysis of posttranslational protein arginylation. PLoS Biol. 5, e258 (2007).
Lee, M. J. et al. RGS4 and RGS5 are in vivo substrates of the N-end rule pathway. Proc. Natl Acad. Sci. USA 102, 15030–15035 (2005).
Piatkov, K. I., Brower, C. S. & Varshavsky, A. The N-end rule pathway counteracts cell death by destroying proapoptotic protein fragments. Proc. Natl Acad. Sci. USA 109, E1839–E1847 (2012).
Hu, R. G. et al. Arginyltransferase, its specificity, putative substrates, bidirectional promoter, and splicing-derived isoforms. J. Biol. Chem. 281, 32559–32573 (2006).
Cha-Molstad, H. et al. Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding. Nat. Cell Biol. 17, 917–929 (2015).
Yoshida, S., Ito, M., Callis, J., Nishida, I. & Watanabe, A. A delayed leaf senescence mutant is defective in arginyl-tRNA:protein arginyltransferase, a component of the N-end rule pathway in Arabidopsis. Plant J. 32, 129–137 (2002).
Schuessele, C. et al. Spatio-temporal patterning of arginyl-tRNA protein transferase (ATE) contributes to gametophytic development in a moss. New Phytol. 209, 1014–1027 (2016).
Hoernstein, S. N. et al. Identification of targets and interaction partners of arginyl-trna protein transferase in the moss Physcomitrella patens. Mol. Cell Proteomics 15, 1808–1822 (2016).
Dar, M. H., de Janvry, A., Emerick, K., Raitzer, D. & Sadoulet, E. Flood-tolerant rice reduces yield variability and raises expected yield, differentially benefitting socially disadvantaged groups. Sci. Rep. 3, 3315 (2013).
Petra Majovsky, Domenika Thieme and Wolfgang Hoehenwarter from the Proteomics Unit of the Leibniz Institute of Plant Biochemistry (IPB), Halle, are acknowledged for MS of recombinant ATE1. David Staunton from the Biophysical Facility, Department of Biochemistry, University of Oxford, is acknowledged for multi-angle light scatter analysis of PCO1/4. Geoff Grime from the University of Surrey Ion Beam Centre is acknowledged for assistance with the microPIXE data collection. This work was supported by a Biotechnology and Biological Sciences Research Council (U.K.) New Investigator grant (BB/M024458/1) to E.F., a grant for setting up the junior research group of the ScienceCampus Halle–Plant-based Bioeconomy to N.D., by a PhD fellowship of the Landesgraduiertenförderung Sachsen-Anhalt awarded to C.N., by an Engineering and Physical Sciences Research Council (U.K.) studentship (EP/G03706X/1) to J.C.B.-B., a Royal Society Dorothy Hodgkin Fellowship to E.F., a William R. Miller Junior Research Fellowship (St Edmund Hall, Oxford) to R.J.H. and grant DI 1794/3-1 by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) to N.D. Financial support came from the Leibniz Association, the state of Saxony Anhalt, the Deutsche Forschungsgemeinschaft (DFG) Graduate Training Center GRK1026 ‘Conformational Transitions in Macromolecular Interactions’ at Halle, and the Leibniz Institute of Plant Biochemistry (IPB) at Halle, Germany. We thank Professor J. van Dongen (RWTH Aachen University, Germany) and Professor F. Licausi (Scuolo Superiore Sant’Anna, Pisa, Italy) for sharing pDEST-PCO plasmids and helpful discussions. The manuscript was deposited as pre-print before publication (https://doi.org/10.1101/069336) at bioRxiv—the preprint server for biology, operated by Cold Spring Harbor Laboratory (biorxiv.org). Publication of this article was funded by the Open Access fund of the Leibniz Institute of Plant Biochemistry (IPB). This work was supported by the network of the European Cooperation in Science and Technology (COST) Action BM1307—‘European network to integrate research on intracellular proteolysis pathways in health and disease (PROTEOSTASIS)’.
The authors declare no competing financial interests.
Supplementary Figures, Supplementary Tables and Supplementary References (PDF 786 kb)
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
White, M., Klecker, M., Hopkinson, R. et al. Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets. Nat Commun 8, 14690 (2017). https://doi.org/10.1038/ncomms14690
This article is cited by
Iron-sulfur clusters are involved in post-translational arginylation
Nature Communications (2023)
Cyanide replaces substrate in obligate-ordered addition of nitric oxide to the non-heme mononuclear iron AvMDO active site
JBIC Journal of Biological Inorganic Chemistry (2023)
An oxygen-sensing mechanism for angiosperm adaptation to altitude
RNA- and miRNA-interference to enhance abiotic stress tolerance in plants
Journal of Plant Biochemistry and Biotechnology (2022)
Heat stress induced arginylation of HuR promotes alternative polyadenylation of Hsp70.3 by regulating HuR stability and RNA binding
Cell Death & Differentiation (2021)
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.