The deployment of multidrug efflux pumps is a powerful defence mechanism for Gram-negative bacterial cells when exposed to antimicrobial agents. The major multidrug efflux transport system in Escherichia coli, AcrAB–TolC, is a tripartite system using the proton-motive force as an energy source. The polyspecific substrate-binding module AcrB uses various pathways to sequester drugs from the periplasm and outer leaflet of the inner membrane. Here we report the asymmetric AcrB structure in complex with fusidic acid at a resolution of 2.5 Å and mutational analysis of the putative fusidic acid binding site at the transmembrane domain. A groove shaped by the interface between transmembrane helix 1 (TM1) and TM2 specifically binds fusidic acid and other lipophilic carboxylated drugs. We propose that these bound drugs are actively displaced by an upward movement of TM2 towards the AcrB periplasmic porter domain in response to protonation events in the transmembrane domain.
Bacteria deploy several mechanisms to combat invasion of antimicrobial compounds1. One of the first defence mechanisms is to avoid the accumulation of these toxic agents inside the cytoplasm or periplasm to concentrations lethal to the cell. In Gram-negative bacteria, downregulation of porin production is often used to slow down the antibiotic entry into the periplasm or cytoplasm2. In addition, upregulation of multidrug efflux pumps increases the effect of reducing the drug concentration in these compartments3. In Gram-negative bacteria, many of these transporters are part of so-called tripartite systems that allow the extrusion of substrates across the entire cell envelope, including the transport across the outer membrane4. The AcrAB–TolC complex from Escherichia coli is the model tripartite transporter system. The inner membrane protein AcrB is a member of the Resistance Nodulation cell Division (RND) transporter superfamily and uses the proton motive force to expel drugs in a drug/H+ antiport manner across the membrane. It forms a complex with the periplasmic membrane fusion protein AcrA and the outer membrane factor TolC. AcrB recognizes a wide variety of substrates covering various classes of hydrophobic antibiotics, dyes, detergents, organic solutes and even bile salts5,6, but also carboxylated drugs such as fusidic acid and β-lactams.
Within the homotrimeric setup, each AcrB protomer comprises a large periplasmic domain and a transmembrane (TM) domain with 12 TM-spanning helices (TM1–12) that form a 10-helix bundle around 2 core helices TM4 and TM10 (Fig. 1). The latter two helices contain three essential residues (D407, D408 and K940, another essential residue, R971, resides on TM11) important for H+ binding, H+ transport and energy transduction7,8,9. The TM domain consists of structural parallel repeats, R1 and R2, each comprising five helix bundles. The amino-terminal repeat, R1, includes TM1 and TM3 to TM6, while the carboxy-terminal repeat, R2, comprises TM7 and TM9 to TM12 (Fig. 1)10. Each repeat is flanked by a single TM helix that seems to function as a coupling element with the periplasmic porter domain (Fig. 1). The flanking helices are TM2 and TM8, respectively; TM2 is linked to the flexible PN2/PC1 repeat in the porter domain, while TM8 is connected to the more rigid PN1/PC2 unit (Fig. 1). The large periplasmic part, subdivided into a porter and a funnel domain, consists of two loops located between TM1 and TM2, and between TM7 and TM8 and comprises structural repeats of two similar α/β subdomains (PN1, PN2, PC1 and PC2) joined by a common β-strand (Fig. 1).
On basis of structural and functional studies, the drug specificity filter of the AcrAB–TolC efflux pump resides in the AcrB periplasmic porter domain (Fig. 1)11,12,13. Several drug substrates of AcrB have been shown to bind to two areas within the porter domain, known as the access pocket and deep binding pocket14,15. Structures of asymmetric AcrB14,15,16,17,18,19 revealed binding of minocycline (pdb entries: 2DRD16, 3AOD15, 4DX514), doxorubicin (pdb entries: 2DR6 (ref. 16), 4DX7 (ref. 14)), erythromycin (pdb entry: 3AOC15), rifampicin (pdb entries: 3AOB, 3AOD15), rhodamine 6G (pdb entry: 5ENS20), or pyridopyrimidine/pyranopyridine inhibitors (pdb entries: 3W9H19, 5ENO, 5ENP, 5ENQ, 5ENR20) in either or both the access and deep binding pockets in the porter domain (Fig. 1b). Both periplasmic binding areas are situated along putative drug transport pathways comprised by the individual functional states L, T and O. Current hypothesis describes that these three states can be adopted by any of the protomers constituting the AcrB trimer. That is, when the trimer is actively pumping, the AcrB protomers cycle through the three states L, T and O17 (or access, binding, extrusion16) in a consecutive manner. It has been proposed that high molecular weight drugs (like rifampicin, erythromycin and doxorubicin dimers) are taken up from the periplasm via the access binding site in the L state from where they can reach the deep binding pocket upon transition to the T state towards the interior of the porter domain14,15. Low molecular weight drugs might enter the periplasmic domain via direct pathways towards the deep binding pocket15. Upon the energy-dependent T to O transition, the deep binding pocket collapses, squeezing drugs out, which are subsequently guided through an exit tunnel towards the TolC channel. While this mechanism only explains sequestering of drugs from the periplasmic space, it has been postulated for drugs located in the E. coli cytoplasm, that single component drug /H+ antiporters like EmrE21 and/or MdfA22 translocate the drugs to the periplasm from where these can be further transported by the tripartite RND system as described above23. For specific drugs like fusidic acid and β-lactams, which partition into the outer leaflet of the inner membrane6, however, no other E. coli transporter except RND-type transporters have been shown to be involved in the resistance phenotype24 and their transport route via AcrB is elusive.
Here, we present an asymmetric structure of AcrB in complex with fusidic acid. Substrate binding appears to be mediated by a groove constituted by TM1 and TM2. This groove is pseudosymmetric to the TM7/TM8 groove of the same protomer, the latter postulated to be a binding site for drugs from within the outer leaflet of the inner membrane25 and is located proximal to the predicted tunnel entrance leading to the deep binding pocket5,18.
In this report, substitution analysis by site-directed mutagenesis of those residues comprising the putative fusidic acid binding site in the TM1/TM2 groove render the cells harboring the variant AcrB pumps less effective against fusidic acid and other hydrophobic substrates containing carboxylic acid moieties. Moreover, fusidic acid and dicloxacillin could effectively protect the single cysteine AcrB variant C338 against cross-linking with sulforhodamine methanethiosulfonate (MTS-rhodamine) in a concentration-dependent manner, supporting the role of TM1/TM2 as binding site for these drugs. From the results presented here, we hypothesize that movement of transducing helix TM2 perpendicular to the membrane plane during functional protomer cycling, facilitates the displacement of fusidic acid towards the AcrB porter domain.
Binding of fusidic acid to the TM1–TM2 region
Well-diffracting co-crystals of AcrB/DARPin in complex with fusidic acid were obtained under conditions with 100-fold molar excess of this compound over AcrB. The wild-type AcrB structure (pdb entry: 4DX5) was refined against the 2.5 Å native data set with resulting R/Rfree of 22.5 and 26.2, respectively (Supplementary Table 1). The electron densities derived by simulated annealing composite omit procedure (to reduce model bias) displayed an additional unaccounted electron density (Fig. 2a) located next to TM1 (I27) and TM2 (K334, I337, H338 and V341) of the T conformer at a position ∼6 Å from the periplasmic side of the lipid bilayer. We assigned this electron density to a fusidic acid molecule (Fig. 2b).
Further inspection of the electron density maps in the same area of TM1 and TM2 in the L and O conformers led to identification of similar shaped, albeit less pronounced, electron densities. To obtain more distinct information of fusidic acid binding to the individual protomers, we co-crystallized the AcrB/DARPin complex with 24-bromofusidic acid (Supplementary Fig. 1a) and took advantage of the strong anomalous signal of the bromine atom at the K-edge (0.92 Å). We detected clear anomalous densities at the anticipated TM1/TM2-binding groove of the T and O protomers (Supplementary Fig. 1b,c) but no anomalous signal density at the L protomer. Overall, the Fo−Fc densities of 24-bromofusidic acid were much weaker compared with that of the fusidic acid AcrB complex data set, indicating an overall less affine binding of 24-bromofusidic acid. However, the strong anomalous signals at the bromine edge clearly indicated the presence of the fusidic acid derivative at the T and O protomers located close to the C-24 of fusidic acid (Supplementary Fig. 1b,c), as expected. We also conducted a polder map calculation27 of the TM1/TM2 groove area. Here, clear densities are visible in the L and T protomer at the TM1/TM2 groove, whereas the density at the O protomer is not attributable to fusidic acid (Supplementary Fig. 2), in contrast to the clear binding observed for 24-bromofusidic acid (Supplementary Fig. 1c). As the density derived from the AcrB/fusidic acid complex and the 24-bromofusidic acid/AcrB complex data were complementary in the assignment of the bound molecules to the asymmetric AcrB trimer, we tentatively modelled one fusidic acid molecule per protomer within the asymmetric AcrB trimer structure (Supplementary Fig. 3). Based on the SA omit map and anomalous density analysis (Fig. 2 and Supplementary Figs 1 and 2), we consider the groove at the T protomer as the primary binding site, whereas the binding sites at the O and L protomers appear to have much less affinity for both fusidic acid and 24-bromofusidic acid.
The model containing three fusidic acid molecules at each of the three protomers revealed interactions of fusidic acid with residues I27 on TM1 and K334, I337, H338 and V341 on TM2 (Supplementary Figs 3 and 4a). Distances between ligand and amino acid side chains varied between 2.8 and 4.0 Å. These interactions are mainly of hydrophobic nature, with the exception of a hydrogen bond (2.8–3.2 Å distance) between His338 and the carboxylate moiety of fusidic acid (Supplementary Fig. 4a). The latter interaction is of special interest, as Poisson–Boltzmann calculations predicted different protonation probabilities for His338 in the L (50% probability), T (deprotonated) and O (protonated) state10. Moreover, structural and molecular simulation data indicated a large water accessibility for this TM1/TM2 groove His338/Glu346 region (Fig. 2), most likely to be induced by the L to T transition on ligand binding to the deep binding pocket in the periplasmic porter domain (Fig. 1)10. As His338 is predicted to be deprotonated in the T state, we assume that the carboxyl acid moiety of fusidic acid is likewise neutralized by protonation, and that the interaction with His338 is by hydrogen bonding only. The weaker electron densities found for the putative fusidic acid molecules localized at the same position at the L and O protomers compared with the densities observed for fusidic acid at the T protomer might reflect lower binding affinity due to the different protonation states of the His338 at each protomer10.
We also observed clear additional electron densities in each protomer between TM1 and TM2 next to the fusidic acid molecule. We assigned these densities to dodecyl-β-D-maltoside (DDM; Fig. 3 and Supplementary Figs 4b and 5). Of note, in the previous published structures14 (pdb entries 4DX5 and 4DX7), DDM was assigned to this position as well (in the L and T protomers).
In the distal part of the TM1/TM2 groove, towards the middle of the lipid bilayer, the DDM alkyl chain is located at 6–9 Å distance to three methionine side chains (M20 on TM1, M395 and M398 on TM2; Fig. 3). Lipid alkyl chain interactions in TM regions of membrane proteins are often mediated by methionine residues28, which might indicate that in vivo, a lipid molecule (with two longer alkyl chains) occupies the TM1/TM2 groove of AcrB. In addition to the side chain interactions, the assigned DDM molecules appear to interact with fusidic acid via their alkyl chains (Supplementary Fig. 4b). Interestingly, the orientation of the maltoside headgroup in the T state is markedly different as that in the L and O states. Whereas in the latter two states the maltose moiety shows a single interaction with the L28 carbonyl oxygen, the headgroup is clamped to the protein by multiple hydrogen bonding interaction with PC1 subdomain-derived Asn298 and Asp301, and TM2 located Lys334 in the T state (Supplementary Fig. 4b). The alignment of the DDM appears to intensify its interaction with the dimethylallyl group of fusidic acid and might be an explanation for the more defined electron densities, which we assigned to fusidic acid in the T state. We speculate that in vivo, additional lipid-mediated hydrophobic interaction might stabilize the interaction between fusidic acid and AcrB inside the TM domain. In vitro, however, the exchange of lipid by detergent results in a possible detergent-mediated hydrophobic interaction as seen in the current complex structure.
The observed binding of fusidic acid to each of the three protomers is markedly different from earlier reported co-crystal structures29,30,31,32. These previous structures (at 3.5–3.8 Å resolution) represent the symmetric conformation of AcrB (that is, LLL conformation) and indicated binding of rhodamine 6G, ethidium, dequalinium, ciprofloxacin29, ampicillin30 or linezolid31 molecules in the central cavity comprised by the TM domain of each protomer (Supplementary Fig. 6).
In comparison, the protomers of the asymmetric AcrB structure at high resolution (2.5 Å), reported here, bind fusidic acid at the TM1/TM2 groove, outside the central cavity.
I337 is important for fusidic acid and β-lactam resistance
To correlate the observation of fusidic acid binding to TM2 with the role of this binding site in efflux function, we set out to substitute the main interaction sites I337, H338 and V341 with Ala (and in addition H338 to Arg).
The D407N, I337A, H338A, H338R and V341A substitution variants were equally well-expressed compared with wild-type AcrB from the same expression vector (Fig. 4a). We conducted fusidic acid susceptibility assay with E. coli BW25133ΔacrB cells harbouring either the wild-type acrB gene, the inactive variant acrB_D407N gene9,33 or one of the four substitution mutants using solid growth lysogeny broth (LB) supplemented with fusidic acid (11 μg ml−1). Although substitution of H338 and V341 show no apparent influence on the susceptibility of E. coli in the presence of fusidic acid, a pronounced effect was observed with E. coli containing the AcrB I337A substitution variant (Fig. 4b). Apparently, substitution of H338 with Ala or Arg was without severe consequence on fusidic acid or β-lactam efflux activity, whereas I337 was most sensitive to replacement for these substrates.
Analysis of growth of the substitution variants (Supplementary Fig. 7) on LB liquid medium containing fusidic acid (60 μg ml−1) exhibited inhibited growth of all variants compared with E. coli wild-type containing AcrB. The most severe growth inhibition was observed with the D407N substitution, as expected. From the putative TM2-binding site substitution variants, the I337A variant showed most severe growth inhibition. The severity of growth inhibition for all variants was D407N>I337A>H338R>V341A>H338A>wild-type. On basis of the localization of bound fusidic acid and its interaction with His338, we initially hypothesized that drugs with carboxylic acid moieties might be most affected by substitutions in the TM2, especially the H338 to Ala or Arg substitutions. We therefore included different β-lactam antibiotics (oxacillin, cloxacillin, dicloxacillin and piperacillin) as well as erythromycin, TPP+, linezolid, chloramphenicol, novobiocin, taurocholate, deoxycholate and rhodamine 6G (as controls) in the resistance assay on drug agar plates (Fig. 4c and Supplementary Fig. 8). Again, to our surprise, the I337A substitution variant was most growth inhibited of all substitution variants in the presence of drugs with carboxylic acid moieties, including all four β-lactam substrates, whereas no inhibiting effect could be observed compared with wild-type AcrB in the presence of the other tested substrates (Fig. 4c and Supplementary Fig. 8). The other putative TM2-binding site substitution variants showed little (for example, for V341A in the presence of oxacillin, cloxacillin, or piperacillin) or no growth inhibition in the presence of any of the substrates tested, with the exception of the growth inhibition in the presence of chloramphenicol for the V341A substitution variant (Supplementary Fig. 8).
TM1–TM2 site protection by dicloxacillin and fusidic acid
To verify the observed binding of fusidic acid to the putative TM1/TM2-binding site, we conducted cross-link protection experiments with a single-Cys AcrB variant (H338C). MTS-rhodamine labelling was done in membrane vesicles containing the AcrB-cl_C338 variant from which it was subsequently purified and subjected to SDS–PAGE and in-gel fluorescence analysis. Ninety percent of AcrB_C338 was modified by MTS-rhodamine (20 μM) within 15 min of incubation of the membranes on ice (Supplementary Fig. 9). Addition of fusidic acid or dicloxacillin to the membrane samples before addition of MTS-rhodamine resulted in a pronounced reduction of fluorescence signal of MTS-rhodamine modified AcrB_C338 in a concentration-dependent manner (Fig. 5a,b). In contrast, addition of linezolid did not have a protective effect against MTS-rhodamine labelling (Fig. 5c). As a control, we used the single cysteine variant V14C, with the modified residue located at the N-terminal part of TM1 near the cytoplasmic side of the TM domain, expected not to play a role in the binding or transport of drugs. MTS-rhodamine reacted with C14 at a similar rate compared with the modification of C338. The presence of dicloxacillin or linezolid at concentrations up to 15 mM did not change the extent of labelling with MTS-rhodamine (Fig. 5b,c). A slight increase of MTS-rhodamine labelling was observed in dependence of the increasing fusidic acid concentration (Fig. 5a). This effect is possibly due to the perturbing effect of fusidic acid to the AcrB-containing membrane, slightly facilitating the reaction of MTS-rhodamine with the thiol group of the V14C variant. Importantly, the effect is the exact opposite of the observation with the H338C variant, where the presence of fusidic acid is protecting against labelling with MTS-rhodamine. If the perturbing effect also would facilitate labelling of C338, then the observed protective effect of fusidic acid against MTS-rhodamine cross-linking is even more remarkable (Fig. 5a). The calculated apparent Ki (inhibition constant of MTS-rhodamine labelling) for fusidic acid and dicloxacillin is ∼2.1 and 2.0 mM, respectively. These results revealed that fusidic acid and dicloxacillin are able to bind at the TM1/2 groove and protect the cysteine at position 338 for modification with MTS-rhodamine.
Carboxylate drugs are considered to be negatively charged at neutral pH (pKa values: oxacillin: 2.7, piperacillin: 3.5, dicloxacillin: 3.75 and fusidic acid: 4.7–5.3); however, due to their hydrophobic moieties, these compounds immerse into the outer leaflet of the inner membrane on entry (from the external medium) into the periplasmic space. Near the membrane surface, the pH is expected to be lower than the bulk pH due to accumulation of surface-associated protons34, shifting the equilibrium towards the protonation of the carboxylic acid moieties and immersion of the drug into the outer leaflet of the inner membrane. Based on the previously determined protonation state of H338 in the T state10, we presume H338 to be deprotonated, whereas the carboxylate ligand is protonated. If so, interaction of fusidic acid, oxacillin, piperacillin or dicloxacillin with H338 is hydrogen bonding-mediated rather than by electrostatic interaction and might explain the lack of a severe effect on efflux activity in the H338 to Ala or Arg substitution variant (Fig. 4c). The key factor for carboxylate drug binding and transport appears to be I337, most probably establishing hydrophobic interactions with fusidic acid or the β-lactams oxacillin, cloxacillin, dicloxacillin and piperacillin. Possibly, the DDM molecule observed in the structure (and speculatively a lipid alkyl chain in vivo) might stabilize substrate binding as well. Despite the rather hydrophobic nature of substrate binding to the TM1/TM2 area, the common denominator of those substrates tested and affected by the I337A substitution is the presence of a carboxylic acid moiety. As the interaction of the carboxyl group of the substrates with H338 does not appear to be supercritical, later stages in the transport pathway, apparently involving I337, might cause the substrate-specific phenotype.
Based on the observed differences in the densities and anomalous signal of 24-bromofusidic acid and the number of different side chain interactions with the ligand in the three asymmetric protomers (Supplementary Fig. 4a), we speculate that the drug binds most tightly in the T conformation.
Previous structural analysis led to the hypothesis that the pseudosymmetric five-TM repeats of the AcrB TM domain undergo a lateral shear motion on protonation of the titratable D407 and D408 residues10. This conformational change also leads to a concomitant coil-to-helix transition in TM8, as well as TM2 helix translation towards the periplasm10. Indeed, superimposition of the AcrB T and O conformers indicates an upward movement of TM2 during the T to O transition of ∼2.5 Å (Fig. 6a,b). In accordance, the bound fusidic acid molecule (Fig. 6d–f) likewise shifts 2.5 Å towards the periplasmic side. This shift causes the movement of fusidic acid from the hydrophobic core of the outer leaflet of the inner membrane to the more hydrophilic headgroup area of the phospholipid membrane/periplasmic space interface. Thus, on T to O transition, due to an upward ‘helix shift’ of TM2, the drug is dragged in part out of the membrane towards the periplasm (Fig. 6). A large part of the fusidic acid alkyl chain is also displaced during this T to O transition from the membrane towards the AcrB periplasmic domain. In the O conformation, where TM2 is in the ‘up’ position, fusidic acid locates slightly below a small hydrophobic cave made up of L300, V32, V333, I337 and the alkyl chain of K334 (Supplementary Fig. 10).
Here, drug-binding affinity might change due to the concurrent movement of TM2 and fusidic acid to a different environment and leads to the release of fusidic acid at the periplasmic side. Once TM2 returns to its down state in the O to L transition, due to the relative motion of the two five-TM repeats of the AcrB TM domain caused by deprotonation of the catalytic D407 and D408 residues10, the binding site is partially re-established and might function as a low-affinity binding site in the L protomer, which on transition to the T state turns into a higher affinity site (Supplementary Fig. 4a).
Further transport of fusidic acid towards the periplasmic binding pockets is difficult to predict on basis of current knowledge. One possibility would be the recently calculated ‘PC1/PN2 down’ pathway26, describing movement of drugs from above the TM1/TM2 groove towards the deep binding pocket. The entrance of this pathway would be pseudosymmetric to the TM7/TM8 groove entrance as part of the vestibule pathway14,18,25.
As is summarized in Fig. 7, carboxylate drugs are postulated to be elevated from the membrane during the T to O transition by means of the TM2 helix upward shift, further transported during the O to L transition via the PC1/PN2 down pathway and bound to the deep binding pocket in the L to T transition. This mechanism is linked directly to the protonation and deprotonation events during the T to O and O to L transitions, and mediated by the up and downward movements of TM2, respectively.
The results presented here give for the first time a direct indication that AcrB drug binding sites can be specific for drugs with defined properties, in this case for carboxylated drugs or drugs containing putative negatively charged moieties.
Bacterial strains and growth conditions
E. coli MachT1 (Life Technologies) cells were used as host for cloning procedures. E. coli C43 (DE3)ΔacrAB35 harbouring pET24acrBHis36 was used for protein overproduction. LB medium and LB agar were used for routine bacterial growth at 37 °C. Kanamycin (Applichem) was used at 50 μg ml−1 (Kan50).
Cloning of acrB gene
The acrB gene was amplified from chromosomal E. coli DNA using acrBfor and acrBrev primers36 (Supplementary Table 2). The amplified acrB gene was cloned into pET24a (Novagen) via NdeI and XhoI restriction sites. The resulting plasmid pET24acrBHis was verified by sequencing (Eurofins).
Synthesis of 24-bromofusidic acid
Bromofusidic acid was custom-synthesized by Basilea Pharmaceutica AG (Basel, Switzerland) and was prepared from fusidic acid (CAS 6990-06-3, Sigma-Aldrich) following a five-step procedure reported in patent WO2005/007669A1. The final product was validated by HPLC, liquid cromatography–mass spectrometry and 1H-NMR methods (Supplementary Methods).
pET24acrBHis36 served as template for site-directed mutagenesis. Amino acid substitution was achieved using the ExSite protocol (Stratagene) with 5’-phosphorylated primers (Supplementary Table 2). Mutations were verified by sequencing (Eurofins).
DARPin protein production and purification
Briefly, E. coli XL1-Blue cells harbouring pQE30-DARPin18 were grown overnight in LB liquid medium supplemented with 50 μg ml−1 kanamycin (LBKan) at 37 °C. Overnight cultures were inoculated into fresh LBKan liquid medium and induction was performed with 0.5 mM isopropyl-β-D-thiogalactoside (final concentration) at OD600 of 0.7. Induced cultures were grown overnight at 37 °C. Cells were harvested by centrifugation and suspended in Buffer A (50 mM Tris pH 7.5, 400 mM NaCl and 10 mM Imidazol). Cells were disrupted by triple passage through a cell disruptor (Constant System, Inc., Northants, UK). Insoluble material was removed by centrifugation at 160,000 g for 1 h. Supernatant was added to Ni-NTA agarose beads (30210, Qiagen) and incubated for 1 h with rotation. Subsequently, Ni-NTA agarose beads were washed with 30 and subsequently 20 column volumes of Buffer B (50 mM Tris pH 7.5, 400 mM NaCl, 20 mM Imidazol, 10% Glycerol) and Buffer C (50 mM Tris pH 7.5, 400 mM NaCl, 50 mM Imidazol and 10% Glycerol), respectively. DARPin proteins were eluted with Buffer D (50 mM Tris pH 7.5, 400 mM NaCl, 250 mM Imidazol and 10% Glycerol).
AcrB protein production and purification
E. coli C43 (DE3) ΔacrAB harbouring pET24acrBHis17,36 was grown overnight in LBKan liquid medium at 37 °C. Overnight cultures were inoculated into fresh LBKan liquid medium, grown till OD600 of 0.8 before induction with 0.5 mM isopropyl-β-D-thiogalactoside (final concentration) and allowed to grow at 20 °C for another 16 h. Cells were harvested by centrifugation and suspended in Buffer A (20 mM Tris pH 8.0, 500 mM NaCl, 2 mM MgCl2 and 0.2 mM diisopropyl fluorophosphate). Cells were disrupted by triple passage through a cell disruptor (Constant System Inc.). Insoluble material was removed by centrifugation at 23,000 g for 15 min. Cell membranes were collected by centrifugation at 160,000 g for 1 h. Subsequently, membranes were suspended in Buffer B (20 mM Tris pH 7.5, 150 mM NaCl, 10 mM Imidazol and 10% Glycerol) and solubilized with 1% dodecyl maltoside (D-97002-C, DDM, Glycon, final concentration) at 4 °C for 1 h. Solubilized membranes were centrifuged at 160,000 g for 30 min. The supernatant was mixed with Ni-NTA agarose beads (30210, Qiagen) and incubated for 1 h with rotation. Subsequently, Ni-NTA agarose beads were washed with 40 and subsequently with 30 column volumes of Buffer B (20 mM Tris pH 7.5, 150 mM NaCl, 20 mM Imidazol, 10% Glycerol and 0.03% DDM) and Buffer C (20 mM Tris pH 7.5, 150 mM NaCl, 40 mM Imidazol, 10% Glycerol and 0.03% DDM), respectively. AcrB protein was eluted with Buffer D (20 mM Tris pH 7.5, 150 mM NaCl, 220 mM Imidazol, 10% Glycerol and 0.03% DDM).
Drug agar plate assay
Drug agar plate assays were performed as previously described44. In short, colonies of E. coli BW25113 ΔacrB harbouring pET24acrBHis wild-type and various mutants were picked and grown overnight in LBKan medium at 37 °C. Dilution of the cultures to OD600 10−1–10−6 were prepared and 4 μl were spotted on an LBKan agar plate supplemented with drug (fusidic acid: 11 μg ml−1, TPP+: 80 mg ml−1, erythromycin: 5 μg ml−1, oxacillin: 20 μg ml−1, cloxacillin: 40 μg ml−1, dicloxacillin: 70 μg ml−1, piperacillin: 0.1 μg ml−1, linezolid: 13 μg ml−1, taurocholate: 6 μg ml−1, deoxycholate: 5 μg ml−1, rhodamine 6G: 25 μg ml−1, chloramphenicol: 0.75 μg ml−1, novobiocin: 8 μg ml−1). Plates were incubated overnight at 37 °C.
Western blot analysis
Whole-cell proteins extracted from E. coli BW25113 ΔacrB harbouring pET24acrBHis wild-type and various variants were resolved by 12.5% SDS–PAGE gels and transferred onto nitrocellullose membrane. The membrane was incubated with anti-AcrB antibody (dilution of 1:10,000; Neosystems, France, custom-antibody) for 90 min. Subsequently, the blot was treated with anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody (dilution of 1:1,500; A3687, Sigma-Aldrich, St. Louis, USA) for 90 min. NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) were used for blot development. Unmodified western blotting of Fig. 4a is shown in Supplementary Fig. 11
Colonies of E. coli BW25113ΔacrB harbouring pET24acrBHis wild-type and various variants were selected and grown in LBKan at 37 °C for 6 h. Cultures were diluted to OD600 of 0.02 and 50 μl was used to inoculate 100 μl of LBKan supplemented with fusidic acid at different concentrations in a 96-well microtitre plate as indicated. The microtitre plate was placed at 37 °C and the OD600 was measured using a Tecan Infinite M200 Reader every 20 min for a period of 11 h.
Substrate protection cross-linking assay
Membranes were prepared as shown above. A membrane aliquot (∼0.1 g) was incubated with 5 mM dithiothreitol for 30 min on ice, followed by two washing steps with ice-cold cross-linking buffer (CLB: 20 mM Tris/Cl pH 7.0 and 0.5 M NaCl) and collection by ultracentrifugation at 137,000 g for 30 min. A total of 5 mg membranes was suspended in 150 μl CLB containing 0–14 mM fusidic acid or other substrates as indicated, and sonicated briefly (amplitude=10%, 2 × 1 s, Sonoplus, Bandelin Electronic, Berlin, Germany), before incubation at 18 °C for 16 h. Subsequently, MTS-rhodamine (20 μM final concentration) was added and the sample was incubated for 15 min on ice. The reaction was stopped by addition of methoxypolyethylene glycol maleimide (2 mM final concentration). Membranes were subsequently washed twice with ice-cold CLB (137,000 g for 20 min), and suspended in CLB containing 1% SDS and 2 mM methoxypolyethylene glycol maleimide before purification of AcrB with HisTrap HP beads on a spin column. Purified AcrB was subjected to SDS–PAGE after which the fluorescence signal was detected on a ImageQuant LAS 4000 (Excitation with Epi-Green (Cy3) and emission filter of 575DF20 (Cy3)) (GE Healthcare BioSciences AB, Uppsala, Sweden) before staining with Coomassie Brilliant Blue. Uncropped images of in-gel fluorescence and Coomassie-stained SDS–PAA gels of Fig. 5 (Lane 1 and Lane 2) are shown in Supplementary Fig. 12 (AcrB-cl_C338) and Supplementary Fig. 13 (AcrB-cl_C14).
Crystallization of AcrB–DARPin–fusidic acid complex
For crystallization purposes, AcrB was mixed with DARPin in a 1:2 molar ratio and incubated on ice for 10 min. Excess DARPin was removed by size-exclusion chromatography (Superose 6, GE Healthcare) with buffer containing 20 mM Tris pH 7.5, 150 mM NaCl and 0.03% DDM. AcrB/DARPin was concentrated and used for hanging drop crystallization at 10–15 mg ml−1. Fusidic acid (4 mM) was added before crystallization. The reservoir contained 50 mM ADA pH 6.9, 5% Glycerol, 5–10% PEG4000 and 150–250 mM ammonium sulfate. Crystals appeared in 1 week and grew to their optimum size (100 × 70 × 200 μm) within additional 1–2 weeks. AcrB/DARPin/24-bromofusidic acid crystals were obtained under the same conditions as described above, with the omission of fusidic acid before soaking the apo-AcrB/DARPin crystals with 8 mM brominated fusidic acid for 5 days.
X-ray diffraction data set analysis and refinement procedure
A data set of P212121 crystals was obtained at beamline X06DA of the Swiss Light Source (Paul Scherrer Institute). Data reduction was done with the XDS package37. Structures were refined using phenix.refine from the PHENIX package27 or using REFMAC5 (ref. 38) and validated with MolProbity39. Model rebuilding was done using COOT40. Over 99% of residues are in favoured regions of the Ramachandran plot and only 0.2% of the residues are outliers. A stereo image of part of the electron 2Fo−Fc density is shown in Supplementary Fig. 14. For superimpositions, the programme SUPERPOSE41 was used. Figures were created using PyMOL (Schrödinger, LLC). AcrB crystals with brominated fusidic acid bound were measured at 0.9187 Å at beamline BM14 (European Synchrotron Radiation Facility, Grenoble, France). Data sets were processed and scaled with the XDS package, the unmerged data was set up with ShelxC42 and the anomalous signal of bromine was located by ANODE43.
The atomic coordinates and structure factors of the wild-type AcrB in complex with DARPins and fusidic acid have been deposited at the Protein Data Bank with accession number 5JMN. The authors declare that all other data supporting the findings of this study are available within the paper and its Supplementary Information files or are available from the corresponding author upon request.
How to cite this article: Oswald, C. et al. Transport of lipophilic carboxylates is mediated by transmembrane helix 2 in multidrug transporter AcrB. Nat. Commun. 7, 13819 doi: 10.1038/ncomms13819 (2016).
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This work was supported by the German Research Foundation (SFB 807, Transport and Communication across Biological Membranes), the DFG-EXC115 (Cluster of Excellence Frankfurt–Macromolecular Complexes) and the German-Israeli Foundation (Grant Number I-1202-248.9/2012). The research leading to these results was conducted as part of the Translocation consortium (www.translocation.eu) and has received support from the Innovative Medicines Joint Undertaking under Grant Agreement Number 115525, resources that are composed of financial contribution from the European Union seventh framework programme (FP7/2007-2013) and EFPIA companies in kind contribution. We are also thankful to the beamline staff at the Swiss Light Source (PXIII, SLS) of the Paul Scherrer Institute in Villigen (Switzerland) and at the European Synchrotron Radiation Facility (BM14, ESRF) in Grenoble (France).
The authors declare no competing financial interests.
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Oswald, C., Tam, H. & Pos, K. Transport of lipophilic carboxylates is mediated by transmembrane helix 2 in multidrug transporter AcrB. Nat Commun 7, 13819 (2016). https://doi.org/10.1038/ncomms13819
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