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Target engagement

Shining a light

Nature Chemical Biology volume 13, pages 133–134 (2017)Cite this article

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The ability to measure the binding of a compound to its intended target in live cells or tissue is a critical parameter for drug discovery. A new method using polarized light microscopy adds to the current toolbox by enabling monitoring of target engagement in vitro and in vivo at single-cell resolution.

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Figure 1: Measuring cellular target engagement of unlabeled drugs by using fluorescence polarization microscopy in combination with companion imaging probes (CIPs).

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  1. Nuffield Department of Medicine, Kilian V.M. Huber is at the Structural Genomics Consortium and the Target Discovery Institute, University of Oxford, Oxford, UK.,

    Kilian V M Huber

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  1. Kilian V M Huber
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Correspondence to Kilian V M Huber.

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The author declares no competing financial interests.

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Huber, K. Shining a light. Nat Chem Biol 13, 133–134 (2017). https://doi.org/10.1038/nchembio.2295

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