Directed evolution is a powerful tool for the development of improved enzyme catalysts. Now, a method that enables an enzyme, its encoding DNA and a fluorescent reaction product to be encapsulated in a gel bead enables the application of directed evolution in an ultra-high-throughput format.
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References
Geysen, H. M., Meloen, R. H. & Barteling, S. J. Proc. Natl Acad. Sci. USA 81, 3998–4002 (1984).
Nielsen, J., Brenner, S. & Janda, K. D. J. Am. Chem. Soc. 115, 9812–9813 (1993).
Boder, E. T. & Wittrup, K. D. Nature Biotechnol. 15, 553–557 (1997).
Smith, G. P. Science 228, 1315–1317 (1985).
Tawfik, D. S. & Griffiths, A. D. Nature Biotechnol. 16, 652–656 (1998).
Yonezawa, M., Doi, N., Higashinakagawa, T. & Yanagawa, H. J. Biochem. 135, 285–288 (2004).
Bertschinger, J. & Neri, D. Protein Eng. Des. Sel. 17, 699–707 (2004).
Figueiredo, P., Roberts, R. L. & Nester, E. W. Proteomics 4, 3128–3140 (2004).
Nord, O., Uhlén, M. & Nygren, P.-Å. J. Biotechnol. 106, 1–13 (2003).
Fischlechner, M. et al. Nature Chem. 6, 791–796 (2014).
Hartwell, L., Mankoff, D., Paulovich, A., Ramsey, S. & Swisher, E. Nature Biotechnol. 24, 905–908 (2006).
Mureev, S., Kovtun, O., Nguyen, U. T. T. & Alexandrov, K. Nature Biotechnol. 27, 747–752 (2009).
Holliger, P. & Hudson, P. J. Nature Biotechnol. 23, 1126–1136 (2005).
Baker, M. Nature Methods 9, 541–544 (2012).
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Wee, E., Trau, M. Evolution made easy. Nature Chem 6, 756–757 (2014). https://doi.org/10.1038/nchem.2046
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DOI: https://doi.org/10.1038/nchem.2046