Modelling organs in culture has great potential to improve our understanding of development, organogenesis and disease. While some endodermal derived organs have been modelled, the corpus region of the stomach, where acid-producing cells reside, remained an invincible target. A 60-day differentiation protocol now enables the generation of functional acid-producing cells in culture, conquering the challenge.
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References
Noguchi, T. K. et al. Nat. Cell Biol. 17, 984–993 (2015).
Lee, E. R., Trasler, J., Dwivedi, S. & Leblond, C. P. Am. J. Anat. 164, 187–207 (1982).
Karam, S. M. & Leblond, C. P. Anat. Rec. 236, 259–279 (1993).
Ootani, A., Toda, S., Fujimoto, K. & Sugihara, H. Biochem. Biophys. Res. Commun. 271, 741–746 (2000).
Katano, T. et al. Biochem. Biophys. Res. Commun. 432, 558–563 (2013).
Li, X. et al. Nat. Med. 20, 769–777 (2014).
Barker, N. et al. Cell Stem Cell 6, 25–36 (2010).
Stange, D. E. et al. Cell 155, 357–368 (2013).
Bartfeld, S. et al. Gastroenterology 148, 126–136 (2015).
McCracken, K. W. et al. Nature 516, 400–404 (2014).
Zorn, A. M. & Wells, J. M. Annu. Rev. Cell Dev. Biol. 25, 221–251 (2009).
D'Amour, K. A. et al. Nat. Biotechnol. 23, 1534–1541 (2005).
Kubo, A. et al. Development 131, 1651–1662 (2004).
Fukuda, K. & Yasugi, S. Dev. Growth Differ. 47, 375–382 (2005).
Ramalho-Santos, M., Melton, D. A. & McMahon, A. P. Development 127, 2763–2772 (2000).
Torihashi, S. et al. Stem Cells 24, 2618–2626 (2006).
Suerbaum, S. & Michetti, P. N. Engl. J. Med. 347, 1175–1186 (2002).
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Huch, M. Building stomach in a dish. Nat Cell Biol 17, 966–967 (2015). https://doi.org/10.1038/ncb3211
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DOI: https://doi.org/10.1038/ncb3211
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