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Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity

Nature Cell Biology volume 10, pages 237244 (2008) | Download Citation



The polarization of yeast and animal cells relies on membrane sterols for polar targeting of proteins to the plasma membrane, their polar endocytic recycling and restricted lateral diffusion1,2,3,4. However, little is known about sterol function in plant-cell polarity5. Directional root growth along the gravity vector requires polar transport of the plant hormone auxin. In Arabidopsis, asymmetric plasma membrane localization of the PIN–FORMED2 (PIN2) auxin transporter directs root gravitropism6,7,8,9,10. Although the composition of membrane sterols influences gravitropism and localization of two other PIN proteins11, it remains unknown how sterols contribute mechanistically to PIN polarity. Here, we show that correct membrane sterol composition is essential for the acquisition of PIN2 polarity. Polar PIN2 localization is defective in the sterol-biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) which displays altered sterol composition, PIN2 endocytosis, and root gravitropism. At the end of cytokinesis, PIN2 localizes initially to both newly formed membranes but subsequently disappears from one. By contrast, PIN2 frequently remains at both daughter membranes in endocytosis-defective cpi1-1 cells. Hence, sterol composition affects post-cytokinetic acquisition of PIN2 polarity by endocytosis, suggesting a mechanism for sterol action on establishment of asymmetric protein localization.

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We gratefully acknowledge M. Bennett, D. Ehrhardt, T. Guilfoyle, J. Haseloff, R. Heidstra, R. Hellens, I. Moore, P. Mullineaux, G. Jürgens, B. Scheres, R. Swarup and J. Xu for sharing published research materials used in this study. We also acknowledge the Nottingham Arabidopsis Stock Centre for distributing mutant lines including SALK T-DNA insertion mutants, provided by J. Alonso and J. Ecker, and the John Innes Centre for EXOTIC Gene Trap lines. We thank I.-B. Carlsson and K. Lundgren for technical assistance with sterol measurements and K. Schumacher for providing seedlings for electron microscopy. We thank L. Bako, R. Bhalerao, U. Fischer, E. Johnson, A. Marchant, and G. Samuelsson for discussions and comments on the manuscript. This work was supported by a grant from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas) to M.G., in part by a postdoctoral stipend from the Carl Tryggers Foundation to Y.B., an EU Marie-Curie International Incoming Postdoctoral Fellowship to Y.I., and the Swedish Foundation for Strategic Research (SSF).

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    • Markus Grebe

    Present address: Umeå Plant Science Centre, Department of Plant Physiology, University of Umeå, SE-90187 Umeå, Sweden.


  1. Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183 Umeå, Sweden.

    • Shuzhen Men
    • , Yohann Boutté
    • , Yoshihisa Ikeda
    • , Thomas Moritz
    •  & Markus Grebe
  2. Institute of Biology II, University of Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.

    • Xugang Li
    •  & Klaus Palme
  3. Centre for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany.

    • York-Dieter Stierhof
  4. Institute for Plant Molecular Biology (IBMP, UPR CNRS 2357), Université Louis Pasteur, 28 rue Goethe, F-67083 Strasbourg, France.

    • Marie-Andrée Hartmann


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Corresponding author

Correspondence to Markus Grebe.

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    Supplementary figures S1, S2, S3, S4, S5, Supplementary table S1 and Supplementary Methods

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