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An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans

Nature Cell Biology volume 6, pages 31–37 (2004)Cite this article

Abstract

In the nematode Caenorhabditis elegans, the gonad acquires two U-shaped arms through the directed migration of its distal tip cells (DTCs), which are located at the tip of the growing gonad arms1. A member of the ADAM (a disintegrin and metalloprotease) family, MIG-17, regulates directional migration of DTCs: MIG-17 is synthesized and secreted from the muscle cells of the body wall, and diffuses to the gonad where it is required for DTC migration2. The mig-23 mutation causes defective migration of DTCs and interacts genetically with mig-17. Here, we report that mig-23 encodes a membrane-bound nucleoside diphosphatase (NDPase) required for glycosylation and proper localization of MIG-17. Our findings indicate that an NDPase affects organ morphogenesis through glycosylation of the MIG-17 ADAM protease.

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Figure 1: Wild-type and mutant gonad morphology.
Figure 2: Structure and activity of MIG-23.
Figure 3: Glycan modification of MIG-17.
Figure 4: Expression of mig-23 and mig-17.

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Acknowledgements

We thank A. Coulson, A. Fire, Y. Kohara, T. Stiernagle and the Caenorhabditis Genetics Center for materials; and M. Lamphier and A. Spence for critical reading of the manuscript. This work was supported by special grants for PRESTO (K. N.), CREST and Advanced Research on Cancer from the Ministry of Education, Culture and Science of Japan (K. M.).

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Authors and Affiliations

  1. RIKEN Center for Developmental Biology and PRESTO, Japan Science and Technology Corporation, Chuo-ku, Kobe, 650-0047, Japan

    Kiyoji Nishiwaki & Yukihiko Kubota

  2. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, AIST Central 6, Tsukuba, 305-8566, Japan

    Yuko Chigira, Samir Kumar Roy & Yoshifumi Jigami

  3. Department of Molecular Biology, Graduate school of Science, Institute for Advanced Research, Nagoya University, and CREST, Japan Science and Technology Corporation, Chikusa-ku, Nagoya, 464-8602, Japan

    Maho Suzuki, Naoki Hisamoto & Kunihiro Matsumoto

  4. Department of Molecular and Medical Genetics, University of Toronto, Toronto, M5S 1A8, Ontario, Canada

    Mara Schvarzstein

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  1. Kiyoji Nishiwaki
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Correspondence to Kiyoji Nishiwaki.

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Nishiwaki, K., Kubota, Y., Chigira, Y. et al. An NDPase links ADAM protease glycosylation with organ morphogenesis in C. elegans. Nat Cell Biol 6, 31–37 (2004). https://doi.org/10.1038/ncb1079

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