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Increased Virulence of Agrobacterium Rhizogenes Conferred by the vir Region of pTiBo542: Application to Genetic Engineering of Poplar

Bio/Technology volume 5, pages 1323–1327 (1987)Cite this article

Abstract

The ability of Agrobacterium rhizogenes to transform plants is dramatically increased by the presence of pTVK291, a plasmid containing part of the vir region of pTiBo542, the “supervirulent” plasmid. We have stably transformed the hybrid Populus trichocarpa x P. deltoides (clone H11) with “supervirulent” A. rhizogenes strains. The use of these bacterial strains as vectors for plant engineering was demonstrated by the transfer to poplar cells of the neomycin phosphotransferase II gene conferring resistance to kanamycin. The applications of these new A. rhizogenes strains to plant biotechnology are discussed.

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Author notes

  1. Vilas P. Sinkar and Milton P. Gordon: Department of Biochemistry, University of Washington, Seattle, WA 98195.

  2. Milton P. Gordon: Corresponding author.

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Washington, Seattle, WA, 98195

    Francois Pythoud, Vilas P. Sinkar, Eugene W. Nester & Milton P. Gordon

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  1. Francois Pythoud
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  2. Vilas P. Sinkar
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  3. Eugene W. Nester
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  4. Milton P. Gordon
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Pythoud, F., Sinkar, V., Nester, E. et al. Increased Virulence of Agrobacterium Rhizogenes Conferred by the vir Region of pTiBo542: Application to Genetic Engineering of Poplar. Nat Biotechnol 5, 1323–1327 (1987). https://doi.org/10.1038/nbt1287-1323

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