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Biosynthesis of Human Tissue-Type Plasminogen Activator by Normal Cells

Bio/Technology volume 2, pages 1058–1062 (1984)Cite this article

Abstract

A method of production of human tissular plasminogen activator (H t-PA) using human embryonic lung (HEL) cells that are devoid of “transforming growth factors” (TGFs) is described. Basal levels of H t-PA in serum-free medium were increased about four times using concanavalin A (Con A) as inducer. After a resting period of 5–7 days, cells could be reinduced by Con A. Three cycles of induction can routinely be performed on the same cell monolayer. H t-PA was purified by a two-step method (affinity chromatography on fibrin and gel filtration). Two molecular forms of H t-PA were obtained, one of 56,000 daltons (the more abundant), and the other of 83,000 daltons.

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Author notes

  1. Gérard C. Brouty-Boye: To whom correspondence should be addressed

Authors and Affiliations

  1. Institut Choay, 10, rue Morel, 92120, Montrouge, France

    Gérard C. Brouty-Boye, Michel Maman & Patrick Choay

  2. Genefusion, 48 bis, rue des Belles-Feuilles, 75016, Paris, France

    Jean-Claude Marian

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  1. Gérard C. Brouty-Boye
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  2. Michel Maman
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  3. Jean-Claude Marian
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  4. Patrick Choay
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Brouty-Boye, G., Maman, M., Marian, JC. et al. Biosynthesis of Human Tissue-Type Plasminogen Activator by Normal Cells. Nat Biotechnol 2, 1058–1062 (1984). https://doi.org/10.1038/nbt1284-1058

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