Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Uninhibited antibiotic target discovery via chemical genetics

Nature Biotechnology volume 22, pages 1528–1529 (2004)Cite this article

A combination of high-throughput screening and multicopy suppression identifies bacterial targets and resistance mechanisms of novel antimicrobials.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Multicopy suppression in a high-throughput screen for novel antimicrobial compounds.

Bob Crimi

References

  1. Miesel, L., Greene, J. & Black, T.A. Nat. Rev. Genet. 4, 442–456 (2003).

    Article  CAS  Google Scholar 

  2. Li, X. et al. Chem. Biol. 11, 1423–1430 (2004).

    Article  CAS  Google Scholar 

  3. Specht, K.M. & Shokat, K.M. Curr. Opin. Cell Biol. 14, 155–159 (2002).

    Article  CAS  Google Scholar 

  4. Stockwell, B.R. Nat. Rev. Genet. 1, 116–125 (2000).

    Article  CAS  Google Scholar 

  5. Poole, K. Clin. Microbiol. Infect. 10, 12–26 (2004).

    Article  CAS  Google Scholar 

  6. Lomovskaya, O. & Watkins, W.J. Curr. Med. Chem. 8, 1699–1711 (2001).

    Article  CAS  Google Scholar 

  7. Lomovskaya, O. & Watkins, W. J. Mol. Microbiol. Biotechnol. 3, 225–236 (2001).

    CAS  PubMed  Google Scholar 

  8. Tegos, G., Stermitz, F.R., Lomovskaya, O. & Lewis, K. Antimicrob. Agents Chemother. 46, 3133–3141 (2002).

    Article  CAS  Google Scholar 

  9. Hsieh, P.C. et al. Proc. Natl. Acad. Sci. USA 95, 6602–6606 (1998).

    Article  CAS  Google Scholar 

  10. Li, X.-Z., Zhang, L. & Poole, K. J. Antimicrob. Chemother. 45, 433–436 (2000).

    Article  CAS  Google Scholar 

  11. Vaara, M. & Nurminen, M. Antimicrob. Agents Chemother. 43, 1459–1462 (1999).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. the Department of Microbiology and Immunology, Queen's University, Kingston, K7L 3N6, Ontario, Canada

    Keith Poole

Authors
  1. Keith Poole
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Poole, K. Uninhibited antibiotic target discovery via chemical genetics. Nat Biotechnol 22, 1528–1529 (2004). https://doi.org/10.1038/nbt1204-1528

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt1204-1528

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing