Abstract
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (μFACS) for sorting various biological entities. Compared with conventional FACS machines, the μFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used μFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
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References
Shapiro, H.M. Practical flow cytometry (Wiley-Liss, New York; 1995 ).
Melamed, M.R., Lindmo, T. & Mendelsohn, M.L. Flow cytometry and sorting (Wiley-Liss, New York; 1990).
Crosland-Taylor, P.J. A device for counting small particles suspended in a fluid through a tube. Nature 171, 37–38 (1953).
Coulter, W. Means for counting particles suspended in a fluid. US 2,656,508 (1949).
Kamentsky, L.A., Melamed, M.R. & Derman, H. Spectrometer: new instrument for ultrarapid cell analysis. Science 150, 630–631 (1965).
Sweet, R.G. High frequency recording with electrostatically deflected ink jets. Rev. Sci. Instrum. 36, 131–133 (1965).
Fulwyer, M.J. Electronic separation of biological cells by volume. Science 150, 910–911 (1965).
Brody, J.P. Valveless liquid microswitch. US 656,155 (1998).
Fiedler, S., Shirley, S.G., Schnelle, T. & Fuhr, G. Dielectrophoretic sorting of particles and cells in a microsystem. Anal. Chem. 70, 1909–1915 (1998).
Li, P.H. & Harrison, D.J. Transport, manipulation, and reaction of biological cells on-chip using electrokinetic effect. Anal. Chem. 69, 1564–1568 (1997).
Carlson, R.H. et al. Self-sorting of white blood cells in a lattice. Physical Review Letters 79, 2149–2152 (1997).
Chou, H.P., Spence, C., Scherer, A. & Quake, S.R. A microfabricated device for sizing and sorting DNA. Proc. Natl. Acad. Sci. USA 96, 11–13 (1999).
Whitesides, G. & Xia, Y. Soft lithography. Angew. Chem. Int. Edn Engl. 37, 550– 575 (1998).
Kopp, M.U., De Mello, A. & Manz, J.A. Chemical amplification: continuous-flow PCR on a chip. Science 280, 1046–1048 (1998).
Harrison, D.J. et al. Micromachining a miniaturized capillary electrophoresis-based chemical-analysis system on a chip. Science 261, 895–897 (1993).
Fodor, S.P.A. et al. Light-directed, spatially addressable parallel chemical synthesis. Science 251, 767–773 (1991).
Acknowledgements
This work was supported in part by the Army Research Office and the National Science Foundation. We especially thank Hou-Pu Chou for fabrication of the silicon molds.
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Fu, A., Spence, C., Scherer, A. et al. A microfabricated fluorescence-activated cell sorter. Nat Biotechnol 17, 1109–1111 (1999). https://doi.org/10.1038/15095
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DOI: https://doi.org/10.1038/15095
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